Results 31 to 40 of about 99,639 (312)

Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction

Veterinary Research, 2017
Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application
Zehui Liu, Yangkun Liu, Yuanyuan Zhang, Yajuan Yang, Jingjing Ren, Xiaoying Zhang, Enqi Du   +6 more
doaj   +1 more source

Studies on processing, particle formation, and immunogenicity of the HIV-1 gag gene product: a possible component of a HIV vaccine [PDF]

, 1992
Antigens in a particulate conformation were shown to be highly immunogenic in mammals. For this reason, the particle forming capacity of derivatives of the HIV-1 group specific core antigen p55 gag was assayed and compared dependent on various expression
Deby, G., Fliessbach, H., Motz, M., Niedrig, M., von Brunn, A., Wagner, R., Wanner, G., Wolf, Hans J.   +7 more
core   +1 more source

Baculovirus as Versatile Vectors for Protein Display and Biotechnological Applications.

Current Issues in Molecular Biology, 2019
The baculovirus-insect cell system has long been deployed for a variety of applications including for use as biopesticides, for recombinant protein production, transient transgene expression, tissue therapy, and for vaccine production.
Chih-Hsuan Tsai, Sung-Chan Wei, Huei-Ru Lo, Y. Chao   +3 more
semanticscholar   +1 more source

Baculovirus Surface Display Using Infuenza Neuraminidase (NA) Transmembrane Anchor

Journal of Tropical Life Science, 2016
Baculovirus surface display has been employed as an excellent tools for presentation of foreign peptides and proteins on virus surface with native conformation, functions and immunogenicity. A baculovirus major envelope protein, gp64, or a capsid protein,
Irisa Trianti, Saengchai Akeprathumchai, Phenjun Mekvichitsaeng, Kanokwan Poomputsa   +3 more
doaj   +1 more source

Recent Developments in the Use of Baculovirus Expression Vectors.

Current Issues in Molecular Biology, 2019
Over 35 years since it was established to make recombinant proteins, the baculovirus expression vector system continues to develop and improve. Early systems for recombinant virus selection were laborious, but better methods were rapidly devised that ...
R. Possee, A. Chambers, Leo P. Graves, Mine Aksular, L. King   +4 more
semanticscholar   +1 more source

Use of the piggyBac transposon to create HIV-1 gag transgenic insect cell lines for continuous VLP production

BMC Biotechnology, 2010
Background Insect baculovirus-produced Human immunodeficiency virus type 1 (HIV-1) Gag virus-like-particles (VLPs) stimulate good humoral and cell-mediated immune responses in animals and are thought to be suitable as a vaccine candidate.
Shephard Enid G, Fraser Malcolm J, Tanzer Fiona, Lynch Alisson G, Williamson Anna-Lise, Rybicki Edward P   +5 more
doaj   +1 more source

A construction strategy for a baculovirus‐silkworm multigene expression system and its application for coexpression of type I and type II interferons

MicrobiologyOpen, 2020
The Bombyx mori nucleopolyhedrovirus (BmNPV) baculovirus expression system (BES) is a eukaryotic expression system. It possesses great capability for post‐translation modification in expression of foreign proteins. With the counterselection cassette rpsL‐
Xingjian Liu, Xin Yang, Arslan Mehboob, Xiaoyuan Hu, Yongzhu Yi, Yinü Li, Zhifang Zhang   +6 more
doaj   +1 more source

MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells

Viruses, 2019
The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics.
K. Gupta, C. Tölzer, Duygu Sari-Ak, Daniel J. Fitzgerald, C. Schaffitzel, I. Berger   +5 more
semanticscholar   +1 more source

Recombinant expression systems: the obstacle to helminth vaccines? [PDF]

, 2007
A
Claerebout, Edwin, De Maere, Veerle, Geldhof, Peter, Vercruysse, Jozef   +3 more
core   +2 more sources

Chemically Defined, High-Density Insect Cell-Based Expression System for Scalable AAV Vector Production

Molecular Therapy: Methods & Clinical Development, 2020
The recombinant adeno-associated virus (AAV) vector is one of the most utilized viral vectors in gene therapy due to its robust, long-term in vivo transgene expression and low toxicity.
James H. Kurasawa, Andrew Park, Carrie R. Sowers, Rebecca A. Halpin, Andrey Tovchigrechko, Claire L. Dobson, Albert E. Schmelzer, Changshou Gao, Susan D. Wilson, Yasuhiro Ikeda   +9 more
doaj   +1 more source