Results 81 to 90 of about 99,639 (312)

A Nymphalid-Infecting Group I Alphabaculovirus Isolated from the Major Passion Fruit Caterpillar Pest Dione juno juno (Lepidoptera: Nymphalidae)

Viruses, 2019
Baculoviruses are capable of infecting a wide diversity of insect pests. In the 1990s, the Dione juno nucleopolyhedrovirus (DijuNPV) was isolated from larvae of the major passionfruit defoliator pest Dione juno juno (Nymphalidae) and described at ...
Bergmann Morais Ribeiro, Ethiane Rozo dos Santos, Luana Beló Trentin, Leonardo Assis da Silva, Fernando Lucas de Melo, Elliot Watanabe Kitajima, Daniel M. P. Ardisson-Araújo   +6 more
doaj   +1 more source

Evaluation of an inducible knockout system in insect cells based on co-infection and CRISPR/Cas9.

PLoS ONE, 2023
Due to comparably high product titers and low production costs, the baculovirus/insect cell expression system is considered a versatile production platform in the biopharmaceutical industry.
Christina Sophie Hausjell, Miriam Klausberger, Wolfgang Ernst, Reingard Grabherr   +3 more
doaj   +1 more source

Expression, Purification, and Microscopy‐Based Assays for Engineered Recombinant Tyrosinated, Detyrosinated, and Δ2 Human Tubulin

Cytoskeleton, EarlyView.
ABSTRACT Microtubules are noncovalent polymers assembled from α/β tubulin dimers. Their structure, dynamics and interaction with effectors are regulated through the expression of diverse tubulin isotypes and chemically diverse posttranslational modifications, also known as the “tubulin code.” Understanding the biophysical correlates between tubulin ...
Jiayi Chen, Agnieska Szyk, Antonina Roll‐Mecak   +2 more
wiley   +1 more source

Baculovirus-Mediated Expression of Human 65 kDa and 67 kDa Glutamic Acid Decarboxylases in SF9 Insect Cells and Their Relevance in Diagnosis of Insulin-Dependent Diabetes Mellitus [PDF]

, 1993
cDNAs coding for the full-length human 65 and 67 kDa glutamic acid decarboxylases (GAD65 and GAD67) were amplified from pancreas and hippocampus cDNA libraries by polymerase chain reaction, respectively. Both cDNAs were inserted into a baculovirus vector
Abney, Charles C., Berthold, Heike, Cook, Neil J., Haubruck, Heinz, Liedvogel, Bodo, Mauch, Ludwig, Northemann, Wolfgang, Scherbaum, Werner A., Seißler, Jochen, Wirbelauer, Christiane   +9 more
core  

Robinow syndrome DVL1 variants disrupt morphogenesis and appendage formation in a Drosophila disease model

Developmental Dynamics, EarlyView.
Abstract Background Robinow syndrome is a rare developmental syndrome caused by variants in genes in Wnt signaling pathways. We previously showed that expression of patient variants in Dishevelled 1 (DVL1) in Drosophila and chicken models disrupts the balance of canonical and non‐canonical Wnt signaling.
Gamze Akarsu, Katja R. MacCharles, Kenneth Kin Lam Wong, Joy M. Richman, Esther M. Verheyen   +4 more
wiley   +1 more source

WSSV ie1 promoter is more efficient than CMV promoter to express H5 hemagglutinin from influenza virus in baculovirus as a chicken vaccine

BMC Microbiology, 2008
Background The worldwide outbreak of influenza A (H5N1) viruses among poultry species and humans highlighted the need to develop efficacious and safe vaccines based on efficient and scaleable production. Results White spot syndrome virus (WSSV) immediate-
Yu Li, Ho YuenFern, He Fang, Kwang Jimmy
doaj   +1 more source

Purification and characterization of recombinant human translation initiation factor eIF3. [PDF]

Protein Sci
Abstract Eukaryotic translation initiation factor 3 (eIF3) is an essential factor in protein synthesis. In mammals, it is a ~800 kDa complex composed of 12–13 subunits. Biochemical and mechanistic studies of the function of eIF3 and its individual subunits in translation require purified eIF3.
Diaz-Lopez I, Gordiyenko Y, Zuber PK, Li X, Ramakrishnan V.   +4 more
europepmc   +2 more sources

Baculovirus infection induces disruption of the nuclear lamina

Scientific Reports, 2017
Baculovirus nucleocapsids egress from the nucleus primarily via budding at the nuclear membrane. The nuclear lamina underlying the nuclear membrane represents a substantial barrier to nuclear egress. Whether the nuclear lamina undergoes disruption during
Xiaomei Zhang, Kaiyan Xu, Denghui Wei, Wenbi Wu, Kai Yang, M. Yuan   +5 more
semanticscholar   +1 more source

Comparison of the Interactions of Transferrin Receptor and Transferrin Receptor 2 with Transferrin and the Hereditary Hemochromatosis Protein HFE [PDF]

, 2000
The transferrin receptor (TfR) interacts with two proteins important for iron metabolism, transferrin (Tf) and HFE, the protein mutated in hereditary hemochromatosis.
Andrews, Nancy C., Bennett, Melanie J., Bjorkman, Pamela J., Enns, Caroline A., Sellers, Vera M., West, Anthony P., Jr.   +5 more
core   +1 more source

Iflaviruses in arthropods: when small is mighty

Insect Science, EarlyView.
Many arthropod species harbor iflaviruses, which often cause covert (asymptomatic) infections, but may still affect host fitness. We review the impact of iflaviruses on arthropod fitness, immunity, behaviour as well as the iflavirus’ host range, transmission, tissue tropism and the interactions with other microorganisms within arthropods.
Annamaria Mattia, Hannah‐Isadora Huditz, Monique M. van Oers, Vera I.D. Ros   +3 more
wiley   +1 more source