Results 191 to 200 of about 1,608,204 (265)

<i>CD1D</i> knockout swine are permissive to African swine fever virus genotype II infection. [PDF]

Emerg Microbes Infect
Madden D, Trujillo JD, Elango S, Fitz I, McDowell CD, Assato P, Lyoo E, Kwon T, Cool K, Li Y, Ferreyra FM, Gaudreault NN, Morozov I, Lee K, Driver J, Richt JA.   +15 more
europepmc   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Comparative osteogenic performance of Chinese hamster ovary cell-derived versus Escherichia coli-derived recombinant human bone morphogenetic protein-2. [PDF]

Bone Joint Res
Matthews C, Johnson L, Holden W, von Benecke J.   +3 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Systemic testosterone induces structural, immunological, and sex steroid receptor changes in human XX skin. [PDF]

Sci Rep
Dabrosin N, Abrahamsson A, Kratz G.
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

The cysteine metabolic regulation through CSE-transsulfuration pathway is essential in iron-induced oxidative damage of heart. [PDF]

Redox Rep
Zhang Y, Xin Y, Zhou J, Yang G, Li S.
europepmc   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Proposal for the Use of an Industrial Membrane System for Lactose Recovery From Whey: Adaptation of Technology Used in Protein Concentration. [PDF]

J Food Sci
Bucior D, Bruel GM, Fernandes IA, Steffens C, Valduga E, Steffens J.   +5 more
europepmc   +1 more source