Cloning and endonuclease restriction analysis of uidA and uidR genes in Escherichia coli K-12: determination of transcription direction for the uidA gene. [PDF]
Journal of Bacteriology, 1982 The two genes of the Escherichia coli K-12 uid region (the structural gene uidA and the regulatory gene uidR) were isolated on a ColE1-uid hybrid plasmid from the bank of Clarke and Carbon. We made a restriction map of this region and correlated it with the genetic map by subcloning the uid restriction fragments into plasmids pBR322, pBR325, and ...
P Ritzenthaler, M Mata-Gilsinger
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Different expression of uidA gene in transformant tobacco leaves
Acta Agriculturae Slovenica, 2002 Agrobacterium-mediated transformation of tobacco leaf disks with two Agrobacterium tumefaciens strains, LBA4404 and EHA105, and four plasmids, pCAMBIA1201, pCAMBIA1301, pCAMBIA2301 and pCAMBIA3201, was used for introduction of an uidA reporter gene and ...
Suzana ŠKOF, Zlata LUTHAR
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Heterogeneity of uidA gene in environmental Escherichia coli populations [PDF]
Journal of Water and Health, 2005 Previous studies have shown that Escherichia coli can be isolated from non-polluted rivers and from bromeliad axilae in pristine areas of tropical rain forests. Finding E. coli in pristine environments is unusual because this bacterium is thought to only survive in the gut of warm-blooded animals and thus its presence should indicate recent fecal ...
Gary Toranzos +2 more
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Eliminating false positives in a qPCR assay for the detection of the uidA gene in Escherichia coli [PDF]
Journal of Water and Health, 2013 Due to contaminant Escherichia coli DNA present in recombinant Taq polymerase reagents, it is not possible to reliably detect low levels of E. coli in samples using the quantitative polymerase chain reaction (qPCR) assay. Native Taq polymerase was successfully used in this study to detect five uidA gene copies (5 fg of genomic DNA) of the uidA gene.
Banu Örmeci +2 more
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Identification of uidA gene sequences in beta-D-glucuronidase-negative Escherichia coli [PDF]
Applied and Environmental Microbiology, 1991 A probe specific for the uidA gene of Escherichia coli hybridized with 112 of 116 E. coli isolates examined, including 31 beta-D-glucuronidase-negative and 12 enterohemorrhagic E. coli serotype O157:H7 isolates. Southern hybridizations confirmed the presence of a 900-bp HinfI fragment from the uidA gene in all isolates examined, suggesting that uidA ...
P Feng
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Genetic analysis of uidA expression in enterohaemorrhagic Escherichia coli serotype 0157:H7 [PDF]
Microbiology (United Kingdom), 1994 Isolates of enterohaemorrhagic Escherichia coli serotype 0157:H7 do not exhibit β-glucuronidase (GUD) activity; however, they carry nucleotide sequences for the uidA gene that encodes the GUD enzyme. Polymerase chain reaction analysis using uidA-specific primers confirmed that a genetic region homologous in size to the E.
Peter Feng, P Feng
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Molecular Plant-Microbe Interactions, 2004 The plant pathogen Erwinia chrysanthemi produces a variety of factors that have been implicated in its ability to cause soft-rot diseases in various hosts.
Clemencia Rojas +2 more
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Serogroups, antibiotic resistance profiles and virulence factors of non-O157 Shiga-toxin producing Escherichia coli from ovine and caprine [PDF]
Scientific ReportsShiga toxin-producing Escherichia coli (STEC) are important zoonotic pathogens linked to foodborne illnesses and outbreaks globally. However, little is known about their prevalence and resistance profiles in small ruminants and their surrounding ...
Jody Howard +3 more
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Virulence Traits and Drug Resistance of STEC Isolated from Layer Poultry and Rattus Species [PDF]
MicroorganismsShiga toxin-producing Escherichia coli (STEC) has emerged globally as a critical enteric foodborne zoonotic pathogen with significant public health implications. This study aimed to isolate and characterize STEC strains from Rattus spp.
Tsepo Ramatla +5 more
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Determination of Antibiotic Resistance Genes in the Interior Bay of Puno-Peru, Lake Titicaca [PDF]
ScientificaWater can serve as a source of genetic resistance and act as an amplifier and/or reservoir for genes acquired by human pathogens, which can be released into the environment as pollutants.
Pompeyo Ferro +6 more
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