Results 21 to 30 of about 3,097 (169)
Zhongguo gonggong weisheng, 2023 ObjectivesTo evaluate the efficiency of detecting six virulence genes of enteroaggregative Escherichia coli (E. coli), abbreviated to EAEC, for diagnosis of EAEC infection among diarrhea patients. MethodsIsolation and identification of E.
Qiqi CUI +5 more
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Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L
Plant, Soil and Environment, 2003 Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively ...
J.I. Flores Solís +3 more
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Rapid detection of Enteroinvasive Escherichia coli by nucleic acid immunochromatography
Zhongguo shipin weisheng zazhi, 2022 ObjectiveTo establish a nucleic acid immunochromatography method for rapid detection of Enteroinvasive Escherichia coli （EIEC）.MethodsThe target single-stranded DNA was prepared by asymmetric PCR， the invE which was the virulence gene of EIEC and uidA ...
ZHAO Xuelong +5 more
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Factors influencing Agrobacterium‐mediated transient expression of uidA in wheat inflorescence tissue [PDF]
Journal of Experimental Botany, 2001 A critical step in the development of Agrobacterium tumifaciens-mediated transformation is the establishment of optimal conditions for T-DNA delivery into tissue from which whole plants can be regenerated. The efficient transformation of inflorescence tissue from 'Baldus', a commercial wheat variety, using the Agrobacterium strain AGLI harbouring the ...
B K, Amoah, H, Wu, C, Sparks, H D, Jones
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Sugarcane (NCo310) Transient Transformation Using uidA Reporter Gene [PDF]
Iranian Journal of Biotechnology, 2013 Background: Sugarcane is a monocotyledonous crop that is cultivated in the tropical and subtropical regions of the world. One of the most important criteria, influencing the efficiency of the sugarcane transformation is known to be related to physical and biological factors during the transformation procedure.
Soheila Matroodi +5 more
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Isolation and characterization of stx1 and stx2 toxin-producing Escherichia coli in neonatal lambs with diarrhea in Nineveh governorate, Iraq [PDF]
Journal of Applied Veterinary Sciences, 2022 Escherichia coli causes many health problems in lambs, the most prominent of which is diarrhea, the study aimed to detect the shiga toxins genes in E.coli isolated from the suckling lambs affected with diarrhea in Nineveh governorate.
A.H. Al-Sabawi, Dh.M. Jwher
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Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein
Biologia Plantarum, 2011 Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm-3 hygromycin. Expression of green fluorescent protein (
C. S. Tee +3 more
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Electronic Journal of Biotechnology, 2018 Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis.
Doribet Jiménez-Guillen +4 more
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â²uidA-antibiotic-resistance cassettes for insertion mutagenesis, gene fusions and genetic constructions [PDF]
FEMS Microbiology Letters, 1992 We have constructed a series of promoterprobe cassettes that provides powerful tools for insertion mutagenesis, transcription fusions and genetic constructions. These cassettes contain the Tn9 chloramphenicol (CmR) and the Tn903 kanamycin (KmR) resistance genes which are expressed in a large variety of microorganisms; these antibiotic-resistance ...
N, Bardonnet, C, Blanco
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Biotecnología Vegetal, 2021 Although genetic transformation in Glycine max has been intensively investigated, the process remains ineffective. In the present work, for the first time, the in vitro selection of transgenic lines of soybean variety Incasoy-27 is addressed.
Jorge L. Pérez Pérez +3 more
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