Results 21 to 30 of about 4,575 (159)

Application of sonication-assisted Agrobacterium-mediated transformation in Chenopodium rubrum L

open access: yesPlant, Soil and Environment, 2003
Chenopodium rubrum belongs to the plant species in which standard Agrobacterium-mediated transformation procedures remain inefficient. We demonstrate that the employment of sonication-assisted Agrobacterium-mediated transformation (SAAT) effectively ...
J.I. Flores Solís   +3 more
doaj   +1 more source

Rapid detection of Enteroinvasive Escherichia coli by nucleic acid immunochromatography

open access: yesZhongguo shipin weisheng zazhi, 2022
ObjectiveTo establish a nucleic acid immunochromatography method for rapid detection of Enteroinvasive Escherichia coli (EIEC).MethodsThe target single-stranded DNA was prepared by asymmetric PCR, the invE which was the virulence gene of EIEC and uidA ...
ZHAO Xuelong   +5 more
doaj   +1 more source

Quantification of human enteric viruses as alternative indicators of fecal pollution to evaluate wastewater treatment processes [PDF]

open access: yesPeerJ, 2022
We investigated the potential use and quantification of human enteric viruses in municipal wastewater samples of Winnipeg (Manitoba, Canada) as alternative indicators of contamination and evaluated the processing stages of the wastewater treatment plant.
Audrey Garcia   +5 more
doaj   +2 more sources

Isolation and characterization of stx1 and stx2 toxin-producing Escherichia coli in neonatal lambs with diarrhea in Nineveh governorate, Iraq [PDF]

open access: yesJournal of Applied Veterinary Sciences, 2022
Escherichia coli causes many health problems in lambs, the most prominent of which is diarrhea, the study aimed to detect the shiga toxins genes in E.coli isolated from the suckling lambs affected with diarrhea in Nineveh governorate.
A.H. Al-Sabawi, Dh.M. Jwher
doaj   +1 more source

Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein

open access: yesBiologia Plantarum, 2011
Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm-3 hygromycin. Expression of green fluorescent protein (
C. S. Tee   +3 more
doaj   +1 more source

In vitro selection of transgenic soybean lines obtained from somatic embryos transformed with Agrobacterium tumefaciens

open access: yesBiotecnología Vegetal, 2021
Although genetic transformation in Glycine max has been intensively investigated, the process remains ineffective. In the present work, for the first time, the in vitro selection of transgenic lines of soybean variety Incasoy-27 is addressed.
Jorge L. Pérez Pérez   +3 more
doaj  

Cloning of the Coffea canephora SERK1 promoter and its molecular analysis during the cell-to-embryo transition

open access: yesElectronic Journal of Biotechnology, 2018
Background: Somatic embryogenesis receptor-like kinase 1 (SERK1) is a cell membrane receptor active in different plant tissues and involved in cell differentiation activities including somatic embryogenesis.
Doribet Jiménez-Guillen   +4 more
doaj   +1 more source

Characterization of the salA, syrF, and syrG Regulatory Genes Located at the Right Border of the Syringomycin Gene Cluster of Pseudomonas syringae pv. syringae

open access: yesMolecular Plant-Microbe Interactions, 2002
Sequence analysis of the right border of the syr gene cluster of Pseudomonas syringae pv. syringae strain B301D revealed the presence of the salA gene 8,113 bp downstream of syrE.
Shi-En Lu   +2 more
doaj   +1 more source

Agrobacterium-mediated UidA gene transformation in finger millet.

open access: yes, 2014
Initially calli were infected with Agrobacterium harboring UidA gene construct and co-cultivated for two days and transferred to the hygromycin selection medium. Photograph of survived calli on selection medium was taken after 30 days (A).
Makarla Udayakumar (153784)   +5 more
core   +1 more source

Effect of some bacterial isolates from G1 and G3B on the DR5::uidA reporter gene for auxin responses in Arabidopsis thaliana.

open access: yes, 2020
(A) Qualitative analysis of uidA gene expression in A. thaliana transgenic lines inoculated with bacterial isolates. Values were obtained by comparing the treatments with the control without inoculation.
José A. Guerrero-Analco (4984826)   +9 more
core   +1 more source

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