Results 41 to 50 of about 3,097 (169)

Rapid Detection of Escherichia coli in Fresh Foods Using a Combination of Enrichment and PCR Analysis

open access: yesFood Science of Animal Resources, 2018
The objective of this study was to determine the minimum enrichment time for different types of food matrix (pork, beef, and fresh-cut lettuce) in an effort to improve Escherichia coli detection efficiency.
Yukyung Choi   +10 more
doaj   +1 more source

Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines Otimização dos parâmetros de bombardeamento de partículas para a transformação genética de linhagens brasileiras de milho

open access: yesPesquisa Agropecuária Brasileira, 2008
The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos.
Caroline Pereira Petrillo   +5 more
doaj   +1 more source

The stability of the Arabidopsis transcriptome in transgenic plants expressing the marker genes nptII and uidA [PDF]

open access: yesThe Plant Journal, 2005
Summary The ATH1 Arabidopsis GeneChip from Affymetrix was used to search for transcriptome changes in Arabidopsis associated with the strong expression of transgenes regulated by constitutive promoters. The insertion and expression of the commonly used marker genes, uidA and
Souad, El Ouakfaoui, Brian, Miki
openaire   +2 more sources

Forest Disturbance Drives Changes in the Functional Traits of Soil Bacteria: A Metagenomic Study of Kauri (Agathis australis) Forest Ecosystems

open access: yesEcology and Evolution, Volume 16, Issue 4, April 2026.
This study reveals how ecological disturbances reshape the structure and function of soil bacterial communities in kauri forests, which are critical for ecosystem resilience. By linking disturbance regimes to shifts in bacterial diversity and functional capacity, it highlights the wider effects of ecosystem disturbance on soil ecological function ...
Alexa K. Byers   +3 more
wiley   +1 more source

Expression of a chimeric uidA gene indicates that polycistronic mRNAs are efficiently translated in tobacco plastids [PDF]

open access: yesThe Plant Journal, 1995
Polycistronic mRNAs are the predominant form of plastid primary transcripts. To determine if there is internal initiation of translation of promoter‐distal open reading frames, a promoterless uidA reporter gene was integrated into the tobacco plastid genome downstream of the rbcL gene.
J M, Staub, P, Maliga
openaire   +2 more sources

Molecular and phenotype characterisation of persistent Escherichia coli in dairy products from Midwest Brazil

open access: yesInternational Journal of Dairy Technology, Volume 79, Issue 2, April–June 2026.
This graphical summary illustrates the isolation and identification of Escherichia coli in samples from the milk production chain. The classical culture methods and molecular methods were used to identify E. coli, followed by the characterisation of biofilm formation, curli production and motility, and verification of pathogenicity and environmental ...
Adriane de Souza Nunes Sikorski   +10 more
wiley   +1 more source

The 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

open access: yesBMC Plant Biology, 2012
Background There are many non-cereal monocots of agronomic, horticultural, and biofuel importance. Successful transformation of these species requires an understanding of factors controlling expression of their genes.
Kamo Kathryn   +3 more
doaj   +1 more source

Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses

open access: yesPlant Biotechnology Journal, Volume 24, Issue 4, Page 2244-2259, April 2026.
ABSTRACT Plant synthetic biology holds great promise for engineering plants to meet future demands. Genetic circuits are being designed, built and tested in plants to demonstrate the proof of concept. However, developing these components in monocots, which the world relies on for grain, lags behind dicot models, such as Arabidopsis thaliana and ...
Dong‐Yeon Lee   +13 more
wiley   +1 more source

Florigen Activation Complex Dynamics and SVP‐Mediated Repression Orchestrate Temperature‐Regulated Flowering in Saffron

open access: yesPlant Biotechnology Journal, Volume 24, Issue 3, Page 1133-1151, March 2026.
ABSTRACT Saffron, a high‐value spice cultivated worldwide for its therapeutic and culinary uses, is a sterile triploid species, rendering conventional breeding approaches ineffective. This limitation underscores the need for molecular and biotechnological strategies for its genetic improvement. Flowering, a key determinant of saffron yield, is strongly
Diksha Kalia   +3 more
wiley   +1 more source

Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors

open access: yesPlant Biotechnology Journal, Volume 24, Issue 3, Page 1269-1284, March 2026.
ABSTRACT RNA interference (RNAi) is a highly conserved gene silencing mechanism regulating gene expression at transcriptional and post‐transcriptional levels in plants. Synthetic trans‐acting small interfering RNAs (syn‐tasiRNAs) have emerged as powerful tools for highly specific and efficient gene silencing.
Ariel H. Tomassi   +8 more
wiley   +1 more source

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