Results 41 to 50 of about 4,575 (159)

Rapid Detection of Escherichia coli in Fresh Foods Using a Combination of Enrichment and PCR Analysis

Food Science of Animal Resources, 2018
The objective of this study was to determine the minimum enrichment time for different types of food matrix (pork, beef, and fresh-cut lettuce) in an effort to improve Escherichia coli detection efficiency.
Yukyung Choi, Sujung Lee, Heeyoung Lee, Soomin Lee, Sejeong Kim, Jeeyeon Lee, Jimyeong Ha, Hyemin Oh, Yewon Lee, Yujin Kim, Yohan Yoon   +10 more
doaj   +1 more source

Optimization of particle bombardment parameters for the genetic transformation of Brazilian maize inbred lines Otimização dos parâmetros de bombardeamento de partículas para a transformação genética de linhagens brasileiras de milho

Pesquisa Agropecuária Brasileira, 2008
The objective of this work was to develop a genetic transformation system for tropical maize genotypes via particle bombardment of immature zygotic embryos.
Caroline Pereira Petrillo, Newton Portilho Carneiro, Antônio Álvaro Corsetti Purcino, Carlos Henrique Siqueira Carvalho, José Donizeti Alves, Andréa Almeida Carneiro   +5 more
doaj   +1 more source

Forest Disturbance Drives Changes in the Functional Traits of Soil Bacteria: A Metagenomic Study of Kauri (Agathis australis) Forest Ecosystems

Ecology and Evolution, Volume 16, Issue 4, April 2026.
This study reveals how ecological disturbances reshape the structure and function of soil bacterial communities in kauri forests, which are critical for ecosystem resilience. By linking disturbance regimes to shifts in bacterial diversity and functional capacity, it highlights the wider effects of ecosystem disturbance on soil ecological function ...
Alexa K. Byers, Leo Condron, Nick Wairapa, Amanda Black   +3 more
wiley   +1 more source

Optimization of physical and biological parameters for transient expression of uidA gene in embryogenic callus of date palm (Phoenix dactylifera L.) via particle bombardment [PDF]

, 2009
The objective of this study was to establish an efficient genetic transformation system in date palm (Phoenix dactylifera L.) using particle bombardment.
Amir Mousavi, Mousa Mousavi, Ali Akbar Habashi, Kazem Arzani, Arzani, K, Mousavi, A, Mousavi, M, Habashi, A   +7 more
core   +1 more source

Molecular and phenotype characterisation of persistent Escherichia coli in dairy products from Midwest Brazil

International Journal of Dairy Technology, Volume 79, Issue 2, April–June 2026.
This graphical summary illustrates the isolation and identification of Escherichia coli in samples from the milk production chain. The classical culture methods and molecular methods were used to identify E. coli, followed by the characterisation of biofilm formation, curli production and motility, and verification of pathogenicity and environmental ...
Adriane de Souza Nunes Sikorski, Adelino Cunha Neto, Elisana Cecilia Nunes Bueno, Márcio Gonçalo de Lima, Samera Rafaela Bruzaroski, Vinicius Silva Castro, Maxsueli Machado, Yuri Duarte Porto, Elsa Helena Walter Santana, Eduardo Eustáquio de Souza Figueiredo, Ricardo César Tavares Carvalho   +10 more
wiley   +1 more source

Transient uidA gene expression in electroporated cotyledonary protoplasts of Pinus nigra ssp. salzmannii and in bombarded cotyledons

, 2000
We have developed a method for the routine isolation of Pinus nigra Arn. ssp. salzmannii (Dunal) Franco protoplasts. The optimized electroporation conditions for uidA gene (gene for beta-glucuronidase) expression in protoplasts from cotyledons excised ...
Marian López, Jaime M Humara, Roberto Rodríguez, Ricardo J Ordás   +3 more
core   +1 more source

Remote Sensing of Endogenous Pigmentation by Inducible Synthetic Circuits in Grasses

Plant Biotechnology Journal, Volume 24, Issue 4, Page 2244-2259, April 2026.
ABSTRACT Plant synthetic biology holds great promise for engineering plants to meet future demands. Genetic circuits are being designed, built and tested in plants to demonstrate the proof of concept. However, developing these components in monocots, which the world relies on for grain, lags behind dicot models, such as Arabidopsis thaliana and ...
Dong‐Yeon Lee, Lucia Acosta‐Gamboa, Luke Saleh, Sunita Pathak, Nathan Swyers, Austin Morgan, Susan Meerdink, Caroline Kuzio, Santiago Calderon, Hudanyun Sheng, Samuel Kenney, Alina Zare, Malia Gehan, Dmitri A. Nusinow   +13 more
wiley   +1 more source

The 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

BMC Plant Biology, 2012
Background There are many non-cereal monocots of agronomic, horticultural, and biofuel importance. Successful transformation of these species requires an understanding of factors controlling expression of their genes.
Kamo Kathryn, Kim Ah-Young, Park Se, Joung Young   +3 more
doaj   +1 more source

Melatonin strongly enhances the Agrobacterium- mediated transformation of carnation in nitrogen-depleted media

BMC Plant Biology, 2023
With the rising demand for new cultivars of carnation, efficient transformation protocols are needed to enable the bioengineering of new traits. Here, we established a novel and efficient Agrobacterium-mediated transformation system using callus as the ...
Omid Aalami, Pejman Azadi, Hanieh Hadizadeh, H. Dayton Wilde, Zahra Karimian, Hossein Nemati, Leila Samiei   +6 more
doaj   +1 more source

Florigen Activation Complex Dynamics and SVP‐Mediated Repression Orchestrate Temperature‐Regulated Flowering in Saffron

Plant Biotechnology Journal, Volume 24, Issue 3, Page 1133-1151, March 2026.
ABSTRACT Saffron, a high‐value spice cultivated worldwide for its therapeutic and culinary uses, is a sterile triploid species, rendering conventional breeding approaches ineffective. This limitation underscores the need for molecular and biotechnological strategies for its genetic improvement. Flowering, a key determinant of saffron yield, is strongly
Diksha Kalia, Joel Jose‐Santhi, Firdous Rasool Sheikh, Rajesh Kumar Singh   +3 more
wiley   +1 more source