Results 51 to 60 of about 4,575 (159)

Expression of the uidA gene controlled by promoters preferentially expressed in the phloem of \'Carrizo\' citrange transgenic plants

open access: yes, 2017
O Brasil é o maior produtor de laranja do mundo, liderando também a produção e exportação de suco concentrado congelado. Um dos fatores que ameaçam o setor é o grande número de doenças, principalmente, as bacterianas como o Huanglongbing (HLB).
Bezerra, Yane Caroline dos Anjos
core   +1 more source

Precision RNAi in Tomato Using Synthetic Trans‐Acting Small Interfering RNAs Derived From Minimal Precursors

open access: yesPlant Biotechnology Journal, Volume 24, Issue 3, Page 1269-1284, March 2026.
ABSTRACT RNA interference (RNAi) is a highly conserved gene silencing mechanism regulating gene expression at transcriptional and post‐transcriptional levels in plants. Synthetic trans‐acting small interfering RNAs (syn‐tasiRNAs) have emerged as powerful tools for highly specific and efficient gene silencing.
Ariel H. Tomassi   +8 more
wiley   +1 more source

Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing

open access: yesThe Plant Journal, Volume 125, Issue 1, January 2026.
Significance Statement A large‐scale mutational analysis revealed that introducing a single G–C base pair upstream of the first DCL1 cleavage site in shc precursors enhances artificial microRNA accumulation and silencing efficiency, providing an optimized amiRNA precursor for improved gene regulation and biotechnological applications.
Juan‐José Llorens‐Gámez   +5 more
wiley   +1 more source

Unravelling the Pathotype, Biofilm, Virulome and Resistome Profile of MultiDrug‐Resistant Bacteria Isolated From Cattle Faeces Using Whole Genome Sequence Analysis

open access: yesInternational Journal of Microbiology, Volume 2026, Issue 1, 2026.
The high mortality and morbidity resulting from diarrhoeal cases worldwide are associated with the increasing incidence of antimicrobial resistance (AMR) and represent a serious public health concern. Cattle are a major reservoir of AMR organisms, and faecal shedding may facilitate their transmission into the food chain.
Tesleem Olatunde Abolarinwa   +10 more
wiley   +1 more source

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

open access: yesPlant Methods, 2018
Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots.
Ezequiel Matias Lentz   +8 more
doaj   +1 more source

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla Organogênese e transformação genética transiente do híbrido Eucalyptus grandis × Eucalyptus urophylla

open access: yesScientia Agricola, 2011
The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E.
Giovana Bomfim de Alcantara   +2 more
doaj   +1 more source

Transient expression of the β-glucuronidase gene in Cannabis sativa varieties

open access: yesPlant Signaling & Behavior, 2020
In plant biology, transient expression analysis plays a vital role to provide a fast method to study the gene of interest. In this study, we report a rapid and efficient method for transient expression in Cannabis sativa seedlings using Agrobacterium ...
Aleksei Sorokin   +3 more
doaj   +1 more source

The Missing Process Hygiene Criteria in the Game Meat Chain: Challenges in Wild Boar Meat Production

open access: yesVeterinary Medicine International, Volume 2026, Issue 1, 2026.
Wild boars are the most widespread large wild mammals globally, capable of reaching high population densities due to their ecological adaptability and human‐related factors. This leads to significant environmental, economic, and public health issues, making effective management essential.
Laura Andriani   +7 more
wiley   +1 more source

Vnos uidA gena v genom tobaka (Nicotiana tabacum L.) z bakterijo Agrobacterium tumefaciens

open access: yes, 2001
Agrobacterium-mediated transformation of tobacco leaf disks with two Agrobacterium tumefaciens (A.t.) strains LBA4404 and EHA105 and four plasmids pCAMBIA1201, pCAMBIA1301, pCAMBIA2301 and pCAMBIA3201 was used for the introduction an  ...
LUTHAR, Zlata, ŠKOF, Suzana
core   +1 more source

Use of direct somatic organogenesis for Agrobacterium-mediated transformation of onion

open access: yesActa Biologica Slovenica, 2003
An Agrobacterium tumefaciens mediated DNA delivery is being developed for onion (Allium cepa L.) using organogenic structures formed on the ovaries as target tissue. An indirect transformation procedure using A.
Manja-Tina Bastar   +2 more
doaj   +1 more source

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