Results 51 to 60 of about 3,097 (169)

Melatonin strongly enhances the Agrobacterium- mediated transformation of carnation in nitrogen-depleted media

open access: yesBMC Plant Biology, 2023
With the rising demand for new cultivars of carnation, efficient transformation protocols are needed to enable the bioengineering of new traits. Here, we established a novel and efficient Agrobacterium-mediated transformation system using callus as the ...
Omid Aalami   +6 more
doaj   +1 more source

Enhanced microRNA accumulation and gene silencing efficiency through optimized precursor base pairing

open access: yesThe Plant Journal, Volume 125, Issue 1, January 2026.
Significance Statement A large‐scale mutational analysis revealed that introducing a single G–C base pair upstream of the first DCL1 cleavage site in shc precursors enhances artificial microRNA accumulation and silencing efficiency, providing an optimized amiRNA precursor for improved gene regulation and biotechnological applications.
Juan‐José Llorens‐Gámez   +5 more
wiley   +1 more source

Carbohydrate-coated magnetic and gold nanoparticles for point-of-use food contamination testing

open access: yesBiosensors and Bioelectronics: X, 2023
With a significant part of the world facing multiple foodborne outbreaks each year, rapid and affordable detection technologies are needed. This paper presents simple equipment-free isolation of bacteria from foods using glycan-coated Magnetic ...
Saad Asadullah Sharief   +2 more
doaj   +1 more source

Transfer of uidA gene into tobacco (Nicotiana tabacum L.) by Agrobacterium tumefaciens

open access: yesActa agriculturae Slovenica, 2001
Agrobacterium-mediated transformation of tobacco leaf disks with two Agrobacterium tumefaciens (A.t.) strains LBA4404 and EHA105 and four plasmids pCAMBIA1201, pCAMBIA1301, pCAMBIA2301 and pCAMBIA3201 was used for the introduction an uidA reporter gene and corresponding plant selection genes (hpt, npt or bar gene) into tobacco (Nicotiana tabacum L. cv.
Suzana ŠKOF, Zlata LUTHAR
openaire   +2 more sources

Unravelling the Pathotype, Biofilm, Virulome and Resistome Profile of MultiDrug‐Resistant Bacteria Isolated From Cattle Faeces Using Whole Genome Sequence Analysis

open access: yesInternational Journal of Microbiology, Volume 2026, Issue 1, 2026.
The high mortality and morbidity resulting from diarrhoeal cases worldwide are associated with the increasing incidence of antimicrobial resistance (AMR) and represent a serious public health concern. Cattle are a major reservoir of AMR organisms, and faecal shedding may facilitate their transmission into the food chain.
Tesleem Olatunde Abolarinwa   +10 more
wiley   +1 more source

Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots

open access: yesPlant Methods, 2018
Aim We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots.
Ezequiel Matias Lentz   +8 more
doaj   +1 more source

Transient expression of uida and gfp genes in Physalis peruviana [PDF]

open access: yesFiziologia rastenij i genetika, 2021
O.M. Yaroshko   +2 more
openaire   +1 more source

The Missing Process Hygiene Criteria in the Game Meat Chain: Challenges in Wild Boar Meat Production

open access: yesVeterinary Medicine International, Volume 2026, Issue 1, 2026.
Wild boars are the most widespread large wild mammals globally, capable of reaching high population densities due to their ecological adaptability and human‐related factors. This leads to significant environmental, economic, and public health issues, making effective management essential.
Laura Andriani   +7 more
wiley   +1 more source

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla Organogênese e transformação genética transiente do híbrido Eucalyptus grandis × Eucalyptus urophylla

open access: yesScientia Agricola, 2011
The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E.
Giovana Bomfim de Alcantara   +2 more
doaj   +1 more source

Molecular Characterisation of Escherichia coli Collected From an Urban River in Johannesburg, South Africa

open access: yesEnvironmental Microbiology Reports, Volume 17, Issue 5, October 2025.
Escherichia coli isolates from the Jukskei River in Johannesburg, South Africa, are genetically diverse, indicating multiple contamination sources and potential cross‐environmental transmission between the river, humans and livestock. Moreover, the presence of pathogenic E.
Luyanda Mkhize   +2 more
wiley   +1 more source

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