Construction and application of an insertional activation cloning vector [PDF]
BMC BiotechnologyBackground Foreign DNA cloning is a critical step in genetic engineering and molecular biology for acquiring target genes. Efficient and accurate identification of positive clones from transformants is essential to this process.
Yan Zhang +4 more
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Safety Assessment of the CP4 EPSPS and NPTII Proteins in Eucalyptus
GM Crops & Food, 2023 Glyphosate herbicide treatment is essential to sustainable Eucalyptus plantation management in Brazil. Eucalyptus is highly sensitive to glyphosate, and Suzano/FuturaGene has genetically modified eucalyptus to tolerate glyphosate, with the aim of both ...
Dror Avisar +24 more
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Paromomycin is a more effective selection agent than kanamycin in Arabidopsis harboring the neomycin phosphotransferase II transgene. [PDF]
PLoS ONENeomycin phosphotransferase II (nptII) is a selectable marker gene that is commonly used in plant molecular genetics and crop improvement, helping researchers to identify and select transgenically modified plants.
Colton P Goodman +3 more
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Prevalence of nptII and Tn5 in kanamycin-resistant bacteria from different environments [PDF]
FEMS Microbiology Ecology, 1993 Kanamycin (Km)-resistant bacterial populations in different soil, river water, sewage and pig manure slurry samples were enumerated and their prevalence in the total populations determined. About 350 Km-resistant Gram-negative colonies grown in the presence of kanamycin were identified using a rapid presumptive identification scheme.
J D Elsas, Smalla K, Van Elsas
exaly +3 more sources
Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells [PDF]
EMBO Journal, 1986 A chimeric gene was constructed containing the light-inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence from Tn5 as a reporter gene (NPTII) and the termination region from chs gene 1 from Petroselinum hortense.
Jeff Schell, Fritz Kreuzaler
exaly +3 more sources
Abundance dynamics and sequence variation of neomycin phosphotransferase gene (nptII) homologs in river water [PDF]
Aquatic Microbial Ecology, 2007 Monitoring the abundance and dynamics of antibiotic resistance genes is essential for assessing their potential effects on environmental and human health. The objectives of the present study were to investigate: (1) the abundance of neomycin phosphotransferase (nptII) gene homologs in water samples collected monthly from the South Saskatchewan River ...
Bo Zhu
exaly +2 more sources
Kanamycin-induced growth suppression of fast-growing transgenic poplar carrying the nptII gene [PDF]
BIO Web of ConferencesIn our study, a genetically modified poplar overexpressing gene encoding gibberellin-20-oxidase (AtGA20ox1) was analyzed for the adaptation of a protocol to maintain in vitro plants due to the overfilling of test tubes during fast-growing transgenic ...
Pavlichenko Vasiliy, Protopopova Marina
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Impact of Exogenous dsRNA on miRNA Composition in Arabidopsis thaliana [PDF]
PlantsThe application of double-stranded RNAs (dsRNAs) to plant surfaces has emerged as a promising tool for manipulating gene expression in plants and pathogens, offering new opportunities for crop improvement.
Nikolay N. Nityagovsky +3 more
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The Specificity of Transgene Suppression in Plants by Exogenous dsRNA
Plants, 2022 The phenomenon of RNA interference (RNAi) is widely used to develop new approaches for crop improvement and plant protection. Recent investigations show that it is possible to downregulate plant transgenes, as more prone sequences to silencing than ...
Konstantin V. Kiselev +5 more
doaj +1 more source
Plants, 2021 Recent studies have revealed that foliar application of double-stranded RNAs (dsRNAs) or small-interfering RNAs (siRNAs) encoding specific genes of plant pathogens triggered RNA interference (RNAi)-mediated silencing of the gene targets.
Konstantin V. Kiselev +4 more
doaj +1 more source