Results 1 to 10 of about 4,031 (171)
Safety Assessment of the CP4 EPSPS and NPTII Proteins in Eucalyptus
GM Crops & Food, 2023 Glyphosate herbicide treatment is essential to sustainable Eucalyptus plantation management in Brazil. Eucalyptus is highly sensitive to glyphosate, and Suzano/FuturaGene has genetically modified eucalyptus to tolerate glyphosate, with the aim of both ...
Dror Avisar +24 more
doaj +6 more sources
Light-induced expression of the chimeric chalcone synthase-NPTII gene in tobacco cells [PDF]
EMBO Journal, 1986 A chimeric gene was constructed containing the light-inducible chalcone synthase (chs) promoter from Antirrhinum majus, the neomycin phosphotransferase structural sequence from Tn5 as a reporter gene (NPTII) and the termination region from chs gene 1 from Petroselinum hortense.
Fritz Kreuzaler
exaly +5 more sources
Evaluation of selection strategies alternative to nptII in genetic transformation of citrus
Plant Cell Reports, 2008 The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants.
A. Ballester +2 more
semanticscholar +5 more sources
Frontiers in Bioengineering and Biotechnology, 2018 Brazil is the largest sugarcane producer and the main sugar exporter in the world. The industrial processes applied by Brazilian mills are very efficient in producing highly purified sugar and ethanol.
Adriana Cheavegatti-Gianotto +7 more
exaly +3 more sources
Prevalence of nptII and Tn5 in kanamycin-resistant bacteria from different environments [PDF]
FEMS Microbiology Ecology, 1993 Kanamycin (Km)-resistant bacterial populations in different soil, river water, sewage and pig manure slurry samples were enumerated and their prevalence in the total populations determined. About 350 Km-resistant Gram-negative colonies grown in the presence of kanamycin were identified using a rapid presumptive identification scheme.
J D Elsas, L S van Overbeek, Van Elsas
exaly +4 more sources
Abundance dynamics and sequence variation of neomycin phosphotransferase gene (nptII) homologs in river water [PDF]
Aquatic Microbial Ecology, 2007 Monitoring the abundance and dynamics of antibiotic resistance genes is essential for assessing their potential effects on environmental and human health. The objectives of the present study were to investigate: (1) the abundance of neomycin phosphotransferase (nptII) gene homologs in water samples collected monthly from the South Saskatchewan River ...
Bo Zhu
exaly +3 more sources
Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica [PDF]
Journal of Agricultural Biotechnology and Sustainable Development, 2014 The present work is an attempt to develop transgenic hairy root system in Camellia assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and
N. Bhattacharyya +4 more
semanticscholar +2 more sources
Genetics and Molecular Research, 2011 In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E.
S. Ghanem
semanticscholar +3 more sources
The Anti-nptII Gene (A Potential Negative Selectable Marker for Plants) [PDF]
Plant Physiology, 1993 An efficient negative selection procedure is crucial to the isolation of rare homologous recombinants in gene targeting. Although gene targeting is a common practice in lower eukaryotes and is becoming routine in mammals, its application to plants has not been achieved.
Cheng-Bin Xiang, D. Guerra
semanticscholar +3 more sources
Survival of Plant Seeds, Their UV Screens, and nptII DNA for 18 Months Outside the International Space Station [PDF]
Astrobiology, 2012 The plausibility that life was imported to Earth from elsewhere can be tested by subjecting life-forms to space travel. Ultraviolet light is the major liability in short-term exposures (Horneck et al., 2001 ), and plant seeds, tardigrades, and lichens-but not microorganisms and their spores-are candidates for long-term survival (Anikeeva et al., 1990 ;
D. Tepfer, Andreja Zalar, S. Leach
semanticscholar +6 more sources