Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica [PDF]
The present work is an attempt to develop transgenic hairy root system in Camellia assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and neomycin phosphotransferase (nptII) as selection marker.
Bhattacharyya Neelakshi +5 more
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Scientific Opinion on an application (EFSA-GMO-NL-2009-70) for the placing on the market of genetically modified drought tolerant maize MON 87460 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto [PDF]
Maize MON 87460 was developed through Agrobacterium-mediated transformation and expresses the cold shock protein B (CspB) from Bacillus subtilis and neomycin phosphotransferase II (NPTII) from Escherichia coli to reduce yield loss under water-limited ...
EFSA Panel on Genetically Modified Organisms (GMO)
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Scientific Opinion on a request from the European Commission for the assessment of the scientific elements put forward by Hungary to support the prohibition for the placing on the market of GM potato EH92-527-1 for cultivation purposes in Hungary [PDF]
Hungary notified to the European Commission its scientific arguments justifying the implementation of a national safeguard measure prohibiting the placing on the market of GM potato EH92-527-1 for cultivation purposes in Hungary, after which the European
EFSA Panel on Genetically Modified Organisms (GMO)
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The effect of different kanamycin concentrations on the growth and morphogenesis of meristematic tissue in the banana “Grand Nain” is presented in this study.
BH Chi-Manzanero +3 more
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Evaluation of selection strategies alternative to nptII in genetic transformation of citrus
The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants.
Ballester, Alida +2 more
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Data on screening and identification of genetically modified papaya in food supplements
This article contains data related to the research article entitled “A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4” (Prins et al ...
Theo W. Prins +6 more
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CRISPR-mediated genome editing in poplar issued by efficient transformation
BackgroundCRISPR has been increasingly used for plant genetic improvements because of its high efficiency and precision. Recently, the authors have reported the possibility of homology-directed repair (HDR) using CRISPR/Cas9 through woody plants such as ...
Ali Movahedi +7 more
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A Biotin-Regulated Locus, bioS, in a Possible Survival Operon of Rhizobium meliloti
A novel biotin-inducible DNA locus, bioS, was found in Rhizobium meliloti 1021 by mutation with a transposable promoter probe. The promoterless nptII reporter responded to 40 nM biotin with a sixfold increase in NPTII protein.
Wolfgang R. Streit, Donald A. Philips
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Отримання генетично-модифікованих рослин пшениці з гетерологічним геном орнітин-δ-амінотрансферази
Мета. Отримання генетично-модифікованих рослин м’якої пшениці з гетерологічним геном орнітин-δ-амінотрансферази. Методи. Agrobacterium-опосередкована трансформація калюсних культур in vitro, ПЛР-аналіз. Результати.
O. M. Honcharuk, O. V. Dubrovna
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Background Species of the fungal genus Trichoderma are important industrial producers of cellulases and hemicellulases, but also widely used as biocontrol agents (BCAs) in agriculture.
Gruber Sabine +4 more
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