Results 21 to 30 of about 12,375 (169)

Agrobacterium mediated transfer of nptII and gus genes in Camellia assamica [PDF]

open access: yesJournal of Agricultural Biotechnology and Sustainable Development, 2014
The present work is an attempt to develop transgenic hairy root system in Camellia assamica, using leaves infected by Agrobacterium rhizogenes strain LBA9402, harboring binary vector (pART27 and pBI121) carrying β-glucuronidase (gus) as reporter gene and neomycin phosphotransferase (nptII) as selection marker.
Bhattacharyya Neelakshi   +5 more
openaire   +1 more source

Scientific Opinion on an application (EFSA-GMO-NL-2009-70) for the placing on the market of genetically modified drought tolerant maize MON 87460 for food and feed uses, import and processing under Regulation (EC) No 1829/2003 from Monsanto [PDF]

open access: yesEFSA Journal, 2012
Maize MON 87460 was developed through Agrobacterium-mediated transformation and expresses the cold shock protein B (CspB) from Bacillus subtilis and neomycin phosphotransferase II (NPTII) from Escherichia coli to reduce yield loss under water-limited ...
EFSA Panel on Genetically Modified Organisms (GMO)
doaj   +1 more source

Scientific Opinion on a request from the European Commission for the assessment of the scientific elements put forward by Hungary to support the prohibition for the placing on the market of GM potato EH92-527-1 for cultivation purposes in Hungary [PDF]

open access: yesEFSA Journal, 2012
Hungary notified to the European Commission its scientific arguments justifying the implementation of a national safeguard measure prohibiting the placing on the market of GM potato EH92-527-1 for cultivation purposes in Hungary, after which the European
EFSA Panel on Genetically Modified Organisms (GMO)
doaj   +1 more source

OPTIMAL CONCENTRATION OF KANAMYCIN AS A SELECTIVE AGENT FOR THE TRANSFORMATION OF Musa cv. “GRAND NAIN”

open access: yesEcosistemas y Recursos Agropecuarios, 2014
The effect of different kanamycin concentrations on the growth and morphogenesis of meristematic tissue in the banana “Grand Nain” is presented in this study.
BH Chi-Manzanero   +3 more
doaj   +1 more source

Evaluation of selection strategies alternative to nptII in genetic transformation of citrus

open access: yesPlant Cell Reports, 2008
The neomycin phosphotransferase (nptII) selection system has proved successful in citrus transformation; however, it may be recommendable to replace it given the pressure exerted against antibiotic-resistance selectable marker genes in transgenic plants.
Ballester, Alida   +2 more
openaire   +3 more sources

Data on screening and identification of genetically modified papaya in food supplements

open access: yesData in Brief, 2016
This article contains data related to the research article entitled “A case study to determine the geographical origin of unknown GM papaya in routine food sample analysis, followed by identification of papaya events 16-0-1 and 18-2-4” (Prins et al ...
Theo W. Prins   +6 more
doaj   +1 more source

CRISPR-mediated genome editing in poplar issued by efficient transformation

open access: yesFrontiers in Plant Science, 2023
BackgroundCRISPR has been increasingly used for plant genetic improvements because of its high efficiency and precision. Recently, the authors have reported the possibility of homology-directed repair (HDR) using CRISPR/Cas9 through woody plants such as ...
Ali Movahedi   +7 more
doaj   +1 more source

A Biotin-Regulated Locus, bioS, in a Possible Survival Operon of Rhizobium meliloti

open access: yesMolecular Plant-Microbe Interactions, 1997
A novel biotin-inducible DNA locus, bioS, was found in Rhizobium meliloti 1021 by mutation with a transposable promoter probe. The promoterless nptII reporter responded to 40 nM biotin with a sixfold increase in NPTII protein.
Wolfgang R. Streit, Donald A. Philips
doaj   +1 more source

Отримання генетично-модифікованих рослин пшениці з гетерологічним геном орнітин-δ-амінотрансферази

open access: yesФактори експериментальної еволюції організмів, 2018
Мета. Отримання генетично-модифікованих рослин м’якої пшениці з гетерологічним геном орнітин-δ-амінотрансферази. Методи. Agrobacterium-опосередкована трансформація калюсних культур in vitro, ПЛР-аналіз. Результати.
O. M. Honcharuk, O. V. Dubrovna
doaj   +1 more source

Generation of Trichoderma atroviride mutants with constitutively activated G protein signaling by using geneticin resistance as selection marker

open access: yesBMC Research Notes, 2012
Background Species of the fungal genus Trichoderma are important industrial producers of cellulases and hemicellulases, but also widely used as biocontrol agents (BCAs) in agriculture.
Gruber Sabine   +4 more
doaj   +1 more source

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