Results 11 to 20 of about 4,031 (171)

The Specificity of Transgene Suppression in Plants by Exogenous dsRNA

Plants, 2022
The phenomenon of RNA interference (RNAi) is widely used to develop new approaches for crop improvement and plant protection. Recent investigations show that it is possible to downregulate plant transgenes, as more prone sequences to silencing than ...
Konstantin V. Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata V. Ogneva, Eduard Y. Kostetsky, Alexandra S. Dubrovina   +5 more
doaj   +2 more sources

Physiological Conditions and dsRNA Application Approaches for Exogenously induced RNA Interference in Arabidopsis thaliana

Plants, 2021
Recent studies have revealed that foliar application of double-stranded RNAs (dsRNAs) or small-interfering RNAs (siRNAs) encoding specific genes of plant pathogens triggered RNA interference (RNAi)-mediated silencing of the gene targets.
Konstantin V. Kiselev, Andrey R. Suprun, Olga A. Aleynova, Zlata V. Ogneva, Alexandra S. Dubrovina   +4 more
doaj   +2 more sources

Genetic engineering for increasing resistance of chickpea (Cicer arietinum L.) to pod borer (Helicoverpa armigera) [PDF]

پژوهش‌های حبوبات ایران, 2010
Pod borer is one of the main factors for yield decrease of chickpea. Therefore, breeding ofchickpea for resistance to this pest is important. Modified cry1Ac gene of Bacillus thuringiensis inpCry1Ac-nptII plasmid containing twin T-DNA for cry1Ac and ...
Nasrin Moshtaghi, Abdol Reza Bagheri, T.J Higgins, Mokhtar Jalali Javaran, Behzad Ghareyazie   +4 more
doaj   +2 more sources

The stability of the Arabidopsis transcriptome in transgenic plants expressing the marker genes nptII and uidA. [PDF]

The Plant Journal, 2005
Summary The ATH1 Arabidopsis GeneChip from Affymetrix was used to search for transcriptome changes in Arabidopsis associated with the strong expression of transgenes regulated by constitutive promoters. The insertion and expression of the commonly used marker genes, uidA and
S. El Ouakfaoui, B. Miki
semanticscholar   +3 more sources

Bioassay and molecular tests of transgenic chickpea (Cicer arietinum L.) resistant to Helicoverpa armigera Hub. [PDF]

پژوهش‌های حبوبات ایران, 2014
Pod borer is one of the main causes for yield loss of chickpea. Therefore, breeding of chickpea for resistance to this pest is important. The use of Cry toxin from Bacillus thuringiensis is an effective strategy for producing of transgenic resistant ...
Parviz Ebadi Babajan, Nasrin Moshtaghi, Abdolreza Bagheri, Hassan Marashi, Saeed Malekzadeh-Shafaroudi   +4 more
doaj   +2 more sources

The positive effect of antibiotic paromomycin compared with kanamycin for selection of transgenic plants with NPTII gene on the example of Nicotiana tabacum

Фактори експериментальної еволюції організмів, 2015
Aim. To investigate the efficiency of antibiotic paromomycin as a selective agent for the production of transgenic plants with nptII gene compared with kanamycin sulfate on the example of Nicotiana tabacum. Methods.
I. O. Nitovska, I. D. Avilov, B. V. Morgun   +2 more
doaj   +2 more sources

No Impact of Transgenic nptII-leafy Pinus radiata (Pinales: Pinaceae) on Pseudocoremia suavis (Lepidoptera: Geometridae) or Its Endoparasitoid Meteorus pulchricornis (Hymenoptera: Braconidae)

Environmental Entomology, 2011
To investigate the biosafety to insects of transgenic Pinus radiata D. Don containing the antibiotic resistance marker gene nptII and the reproductive control gene leafy, bioassays were conducted with an endemic lepidopteran pest of New Zealand ...
E. Burgess, E. Barraclough, A. M. Kean, C. Walter, L. Malone   +4 more
semanticscholar   +2 more sources

Antimicrobial susceptibility patterns of commensal fecal bacteria isolated from pigs with an intentional genomic alteration that included the selectable marker gene nptII

Frontiers in Microbiology
IntroductionAnimals with intentional genomic alterations (IGAs) hold promise for meeting increasing worldwide demand for animal-source proteins. As part of regulatory risk assessment for introducing animals with IGAs into the food chain, monitoring ...
Tessa E. LeCuyer, Jeffrey Monahan, Kayla Farrell, Kent Adams, Anneke Walters, Diamond McClendon, Alexandra Fox, John R. Bianchi   +7 more
doaj   +2 more sources

Field performance of transgenic citrus trees: Assessment of the long-term expression of uidA and nptII transgenes and its impact on relevant agronomic and phenotypic characteristics [PDF]

BMC Biotechnology, 2012
BackgroundThe future of genetic transformation as a tool for the improvement of fruit trees depends on the development of proper systems for the assessment of unintended effects in field-grown GM lines.
E. Pons, Josep E. Peris, L. Peña
semanticscholar   +2 more sources

Kanamycin-induced growth suppression of fast-growing transgenic poplar carrying the nptII gene [PDF]

BIO Web of Conferences
In our study, a genetically modified poplar overexpressing gene encoding gibberellin-20-oxidase (AtGA20ox1) was analyzed for the adaptation of a protocol to maintain in vitro plants due to the overfilling of test tubes during fast-growing transgenic ...
Pavlichenko Vasiliy, Protopopova Marina
doaj   +2 more sources