Functional Validation of the Melon Fom-1 Gene, Controlling Resistance to Fusarium oxysporum Races 0 and 2, by CRISPR/Cas9 Mutagenesis. [PDF]
Mol Plant PatholA CRISPR/Cas9‐induced mutation in an NLR sequence within the melon Prv/Fom‐1 locus abolished resistance to Fusarium oxysporum races 0 and 2, proving the function of an important R‐gene of this crop. ABSTRACT Functional validation of NLR genes is critical for confirming their specific roles and developing durable disease‐resistant crops.
Muthusamy B +3 more
europepmc +2 more sources
A Set of Intein-Split Selectable Marker Genes for Efficient Co-Transformation. [PDF]
Plant Biotechnol JPlant Biotechnology Journal, Volume 24, Issue 4, Page 2439-2441, April 2026.
Moratti FG +6 more
europepmc +2 more sources
Using ngTALEN to improve genome editing efficiency on targets containing 5-methylcytosines. [PDF]
Plant JSUMMARY We recently discovered distinct methylation patterns between the mitochondrial genome and the nuclear‐encoded mitochondrial DNA sequences (NUMTs), with the mitochondrial genome being hypomethylated and NUMTs being hypermethylated. Given that genome editing using mitochondrial targeted transcription activator‐like effector nucleases (TALEN) is ...
Wang Z +5 more
europepmc +2 more sources
Genetic engineering for increasing resistance of chickpea (Cicer arietinum L.) to pod borer (Helicoverpa armigera) [PDF]
پژوهشهای حبوبات ایران, 2010 Pod borer is one of the main factors for yield decrease of chickpea. Therefore, breeding ofchickpea for resistance to this pest is important. Modified cry1Ac gene of Bacillus thuringiensis inpCry1Ac-nptII plasmid containing twin T-DNA for cry1Ac and ...
Nasrin Moshtaghi +4 more
doaj +1 more source
Microorganisms, 2023 The commercialization of industrial genetically modified microorganisms (GMMs) has highlighted their impact on public health and the environment.
Yang Qin, Bo Qu, Bumkyu Lee
doaj +1 more source
Bioassay and molecular tests of transgenic chickpea (Cicer arietinum L.) resistant to Helicoverpa armigera Hub. [PDF]
پژوهشهای حبوبات ایران, 2014 Pod borer is one of the main causes for yield loss of chickpea. Therefore, breeding of chickpea for resistance to this pest is important. The use of Cry toxin from Bacillus thuringiensis is an effective strategy for producing of transgenic resistant ...
Parviz Ebadi Babajan +4 more
doaj +1 more source
The Anti-nptII Gene (A Potential Negative Selectable Marker for Plants) [PDF]
Plant Physiology, 1993 An efficient negative selection procedure is crucial to the isolation of rare homologous recombinants in gene targeting. Although gene targeting is a common practice in lower eukaryotes and is becoming routine in mammals, its application to plants has not been achieved.
C., Xiang, D. J., Guerra
openaire +2 more sources
Bulletin of the National Research Centre, 2022 Background With regard to the extensive production of genetically modified organisms, scientists focused on the safety of antibiotic resistance marker (ARM) genes present in GM-crops produced for the consumption of human or animal.
Hanaa Abdel-Sadek Oraby +3 more
doaj +1 more source
The Iraqi Journal of Agricultural science, 2021 An efficient protocol for Agrobacterium-mediated transformation of tomato cultivars Sandra and Rocky was conducted to examine the possibility of producing transgenic tomato plants cultivars harbouring the nptII gene, conferring kanamycin resistance.
G. H. Danial, D. A. Ibrahim, G. Q. Song
doaj +1 more source
Scientific Opinion on applications (EFSA-GMO-UK-2008-57 and EFSA-GMO-RX-MON15985) for the placing on the market of insect-resistant genetically modified cotton MON 15985 for food and feed uses, import and processing, and for the renewal of authorisation of existing products produced from cotton MON 15985, both under Regulation (EC) No 1829/2003 from Monsanto [PDF]
EFSA Journal, 2014 Cotton MON 15985 was developed by biolistic transformation of cotton MON 531 to express Cry2Ab2 and GUS in addition to the Cry1Ac and NPTII proteins.
EFSA Panel on Genetically Modified Organisms (GMO)
doaj +1 more source