Results 41 to 50 of about 12,375 (169)

Miscanthus giganteus Biolistic Transformation Using the Visible RUBY Red Marker Gene to Monitor Transformation Efficiency

open access: yesGCB Bioenergy, Volume 18, Issue 8, August 2026.
Genetic improvements to Miscanthus × giganteus (M×g), a high‐yielding perennial C4 bioenergy crop, are constrained by triploid sterility and clonal propagation. An efficient biolistic genetic transformation protocol for inflorescence‐derived callus using RUBY, a betalain‐based noninvasive reporter, provides opportunities for crop advancement and tools ...
Balasubramaniam Muthukumar   +8 more
wiley   +1 more source

Survival of Plant Seeds, Their UV Screens, and nptII DNA for 18 Months Outside the International Space Station [PDF]

open access: yesAstrobiology, 2012
The plausibility that life was imported to Earth from elsewhere can be tested by subjecting life-forms to space travel. Ultraviolet light is the major liability in short-term exposures (Horneck et al., 2001 ), and plant seeds, tardigrades, and lichens-but not microorganisms and their spores-are candidates for long-term survival (Anikeeva et al., 1990 ;
Tepfer, David   +2 more
openaire   +5 more sources

Optimisation and Scale‐Up of the Heterologous Production of the Antifungal Protein PeAfpA From Penicillium expansum in Stirred‐Tank Bioreactors

open access: yesMicrobial Biotechnology, Volume 19, Issue 7, July 2026.
This work demonstrates the feasibility of scaling up laboratory processes to pilot scale production of antifungal proteins for diverse applications and exemplifies the possibilities that recyclable CRISPR/Cas9 methodology offers for fungal biotechnology.
Stefani de Ovalle   +6 more
wiley   +1 more source

Отримання трансгенних рослин люцерни (Medicago sativa L.) та арахісу (Arachis hypogaea L.), стійких до гербіциду Pursuit

open access: yesФактори експериментальної еволюції організмів, 2017
Aim. The production of alfalfa and peanut cultivars with new properties is necessary. The purpose of this work was to develop Agrobacterium-mediated transformation protocol and to construct transgenic alfalfa and peanut plants resistant to herbicide ...
S. M. Nifantova   +2 more
doaj   +1 more source

Cross‐Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana‐Derived WUS2 Developmental Regulator

open access: yesPlant Biotechnology Journal, Volume 24, Issue 6, Page 4174-4189, June 2026.
ABSTRACT Plant regeneration is governed by intrinsic gene regulation and phytohormonal cues. WUSCHEL (WUS) gene promotes regeneration, but its broader functional role remains unexplored. Here, we demonstrate that the constitutive and inducible expression of banana‐derived WUS2 (GN‐WUS2) enhances regeneration in Nicotiana tabacum (tobacco) and Musa ...
Roni Chaudhary   +3 more
wiley   +1 more source

Агробактеріальна трансформація in planta пшениці озимого сорту Подолянка та ярого сорту Bobwhite

open access: yesФактори експериментальної еволюції організмів, 2014
Aims. Triticum aestivum L., being one of the world’s most important staple crops, remains a challenge for genetic transformation. This paper describes the application of in planta method to obtain transformants by treating cells of intact plants with ...
І. Р. Горбатюк   +2 more
doaj  

An Agrobacterium mediated transformation system of guava (Psidium guajava L.) with endochitinase gene [PDF]

open access: yesCrop Breeding and Applied Biotechnology, 2014
Genetic transformation of guava (Psidium guajava L.) was developed for the first time using in vitro grown shoot tip explant cocultivated with Agrobacterium tumefaciens strain LBA4404 harbouring binary vector pIIHR-JBMch with endochitinase and nptII ...
Maneesh Mishra   +3 more
doaj  

An efficient and optimized protocol for tall fescue tissue culture and Agrobacterium-mediated genetic transformation

open access: yesJournal of the Saudi Society of Agricultural Sciences, 2023
Festuca arundinacea Schreb. as an important turf widely exposed to biotic and abiotic stresses. Therefore, optimization of an efficient in vitro culture and transformation method to create optimal traits and tolerance against stresses as a major problem,
Hamid Reza Hosseini   +3 more
doaj   +1 more source

Deciphering the biosynthetic pathways of lichen acids

open access: yesNew Phytologist, Volume 250, Issue 3, Page 1784-1799, May 2026.
Summary Depsides and depsidones are polyketide‐derived lichen acids widely distributed in lichen thalli, yet the biosynthetic gene clusters (BGCs) responsible for their production remain poorly understood. To address this gap, we investigated the diversity and evolutionary relationships of polyketide BGCs in lichens.
Wonyong Kim   +11 more
wiley   +1 more source

Evaluation of antibacterial properties of nisin peptide expressed in carrots

open access: yesScientific Reports, 2023
Nisin, derived from Lactococcus lactis, is a well-known natural food preservative. In the present study, the gene of nisin was transformed to carrot by Agrobacterium tumefaciens strain LBA4404 harboring the recombinant binary vector pBI121 containing ...
Masoumeh Fallah Ziarani   +2 more
doaj   +1 more source

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