Results 41 to 50 of about 4,031 (171)

Paromomycin is a more effective selection agent than kanamycin in Arabidopsis harboring the neomycin phosphotransferase II transgene.

PLoS ONE
Neomycin phosphotransferase II (nptII) is a selectable marker gene that is commonly used in plant molecular genetics and crop improvement, helping researchers to identify and select transgenically modified plants.
Colton P Goodman, Victoria N Padgett, Elijah R Evans, Albrecht G von Arnim   +3 more
doaj   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Determination of copy number by Southern hybridization using a NptII (A) or FB_MR5 (B) specific probe.

, 2015
A) A single copy of NptII was detectable in the transgenic motherline (T44.4) and in the vector p9-Dao-FLPi-FB_MR5 (plasmid) when probed with an NptII specific digoxygenin (DIG)-labelled probe. B) Using the FB_MR5 probe an unspecific band was detected in
Andrea Patocchi (103786), Melanie Jänsch (832320), Cesare Gessler (103782), Giovanni A. L. Broggini (479717), Thomas D. Kost (832319), Henryk Flachowsky (832321)   +5 more
core   +1 more source

Agrobacterium tumefaciens-mediated transformation of Jatropha curcas L. with a polyhydroxyalkanoate gene (phaC)

Indonesian Journal of Biotechnology, 2018
Polyhydroxybutyrate is a component of bioplastics that is synthesized under the control of enzymes encoded by pha multigenes. The genes are naturally present in Ralstonia eutropha. However, the production of bioplastics in bacteria is inefficient because
Chesara Novatiano, Adi Pancoro, Erly Marwani   +2 more
doaj   +1 more source

Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme

Plant Biotechnology Journal, EarlyView.
ABSTRACT Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience.
Gulab Chand Arya, Ekram Wassel, Vibha Mishra, Ekaterina Manasherova, Ruth E. Stark, Hagai Cohen   +5 more
wiley   +1 more source

CRISPR/Cas9‐Mediated Knockout of LbSP1 and LbSP5G1 Reveals Efficient Customization of Shoot Architecture in Black Goji Berry

Plant Biotechnology Journal, EarlyView.
ABSTRACT Goji is recognized as a nutritionally rich superfruit with medicinal and industrial importance. However, its inherently indeterminate growth habit causes asynchronous flowering and uneven fruit ripening, resulting in harvesting inefficiency with high labour costs and low fruit yield.
Fazal Rehman, Huanfang Liu, Yun Ma, Shaohua Zeng, Yan Wu, Yuan Zong, Chao Yang, Ying Wang   +7 more
wiley   +1 more source

Schematic diagram of the PpEXP1 over-expression chimeric gene construct, p35S-NPTII/35S-PpEXP1.

, 2014
PpEXP1 is under the control of the CaMV 35S promoter and the kanamycin resistance gene NPTII is the selection marker.
Qian Xu (119888), Bingru Huang (282512), Xiao Xu (112147), Jichen Xu (544619), Yang Shi (175282)   +4 more
core   +1 more source

A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts

Biotechnology for Biofuels, 2018
Background We investigated strategies to improve foreign protein accumulation in the chloroplasts of the model algae Chlamydomonas reinhardtii and tested the outcome in both standard culture conditions as well as one pertinent to algal biofuel production.
Lubna V. Richter, Huijun Yang, Mohammad Yazdani, Maureen R. Hanson, Beth A. Ahner   +4 more
doaj   +1 more source

Harnessing plant‐based platform for low‐cost cellulosic sugar recovery from bioenergy crops

Biofuels, Bioproducts and Biorefining, Volume 20, Issue 5, Page 2106-2121, September/October 2026.
Abstract The price of pure cellulase enzyme for the recovery of fermentable cellulosic sugars is one of the major challenges that limit the commercialization of second‐generation biofuels and bio‐based products. This work shows a means to greatly reduce the cost of cellulases. The abundant capacity of plants to synthesize and hyperaccumulate transgenic
Shraddha Maitra, Kristen Eilts, Raunak Bansal, Stephen P. Long, Vijay Singh   +4 more
wiley   +1 more source

NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana

New Phytologist, Volume 251, Issue 5, Page 2741-2752, September 2026.
Proteomic analysis revealed that receptor kinases (red) accumulate higher in rd21 knockout Nicotiana benthamiana plants. Summary RD21‐like proteases are papain‐like cysteine proteases with a C‐terminal granulin domain that are abundant and ubiquitous in angiosperms and have often been implicated in immunity.
Alice Godson, Lauren Eddie, Mariana Schuster, Kaijie Zheng, Reka Toth, Yuge Li, Tianrun Li, Jie Huang, Farnusch Kaschani, Philippe V. Jutras, Jiorgos Kourelis, Markus Kaiser, Tolga Bozkurt, Renier A. L. Van der Hoorn   +13 more
wiley   +1 more source