Results 51 to 60 of about 4,031 (171)

Отримання трансгенних рослин картоплі (Solanum tuberosum L.), що містять антизмістовну послідовність гена проліндегідрогенази

Фактори експериментальної еволюції організмів, 2018
Мета. Метою роботи було отримання трансгенних рослин картоплі районованих в Україні сортів, у яких проходить експресія дволанцюгового РНК-супресора проліндегідрогенази, що призводить до зменшення деградації проліну і підвищення його концентрації в ...
O. O. Ovcharenko, V. A. Rudas, N. L. Shcherbak, M. V. Kuchuk   +3 more
doaj   +1 more source

Endochitinase Overexpression in Transgenic Tomato Plants Improves Resistance to Phytopathogens

Biotechnology Journal, Volume 21, Issue 8, August 2026.
Graphical Abstract and Lay Summary Transgenic tomato plants overexpressing an endochitinase gene showed confirmed transgene expression and enhanced resistance. Upon plants inoculations with Xanthomonas euvesicatoria pv. perforans and Sclerotinia sclerotiorum, disease severity was significantly reduced compared to wild‐type plants, demonstrating broad ...
Lucas José de Sousa, Ana Carolina Mendes Bezerra, Ivonaldo Reis Santos, Ana Clara Muniz Martins Vilela, Osmundo Brilhante de Oliveira Neto, Glaucia Barbosa Cabral, Alice Maria Quezado‐Duval, Pedro Ricardo Vieira Hamann, Eliane Ferreira Noronha, Luiz Eduardo Bassay Blum, Angela Mehta   +10 more
wiley   +1 more source

Fig 2 -

, 2022
Detection of the transgenic tobacco by PCR analysis: a. SBP-DGAT1 transgenic plants; 1: DGAT1 (1135 bp), 2: nptII (1400 bp), and 3: vir (900 bp) are the PCR products that were synthesized by DGAT1, nptII, and vir forward and reverses primers.
Abbasali Emamjomeh (12769893), Mahmoud Solouki (12769902), Hassan Rahnama (12769896), Nasibeh Chenarani (12769890), Katayoun Zamani (12769899)   +4 more
core   +1 more source

Construction and application of an insertional activation cloning vector

BMC Biotechnology
Background Foreign DNA cloning is a critical step in genetic engineering and molecular biology for acquiring target genes. Efficient and accurate identification of positive clones from transformants is essential to this process.
Yan Zhang, Yun He, Yi Ding, Shanhua Lyu, Yinglun Fan   +4 more
doaj   +1 more source

Most of the Specific Genomic Regions of Agrobacterium fabrum Shape Phenolics Content of Medicago truncatula Roots

Environmental Microbiology Reports, Volume 18, Issue 4, August 2026.
Agrobacterium fabrum harbour seven specific‐gene regions, whose annotation indicates a close connection with plant. To evaluate their involvement in plant‐bacteria interaction, deletion mutant strains of each specific region were inoculated onto Medicago truncatula roots. Root phenolics content was influenced by them.
Rosa Padilla, Ludovic Vial, Florence Dechèvre, Thi Huyen Thu Nguyen, Deniz Sarigol, Vincent Gaillard, Guillaume Meiffren, Gilles Comte, Xavier Nesme, Céline Lavire, Isabelle Kerzaon   +10 more
wiley   +1 more source

Агробактеріальна трансформація in planta пшениці озимого сорту Подолянка та ярого сорту Bobwhite

Фактори експериментальної еволюції організмів, 2014
Aims. Triticum aestivum L., being one of the world’s most important staple crops, remains a challenge for genetic transformation. This paper describes the application of in planta method to obtain transformants by treating cells of intact plants with ...
І. Р. Горбатюк, А. В. Бавол, Б. В. Моргун   +2 more
doaj  

Отримання трансгенних рослин люцерни (Medicago sativa L.) та арахісу (Arachis hypogaea L.), стійких до гербіциду Pursuit

Фактори експериментальної еволюції організмів, 2017
Aim. The production of alfalfa and peanut cultivars with new properties is necessary. The purpose of this work was to develop Agrobacterium-mediated transformation protocol and to construct transgenic alfalfa and peanut plants resistant to herbicide ...
S. M. Nifantova, I. K. Komarnytskyi, M. V. Kuchuk   +2 more
doaj   +1 more source

An efficient and optimized protocol for tall fescue tissue culture and Agrobacterium-mediated genetic transformation

Journal of the Saudi Society of Agricultural Sciences, 2023
Festuca arundinacea Schreb. as an important turf widely exposed to biotic and abiotic stresses. Therefore, optimization of an efficient in vitro culture and transformation method to create optimal traits and tolerance against stresses as a major problem,
Hamid Reza Hosseini, Hassan Salehi, Morteza Khosh-Khui, Mehrangiz Chehrazi   +3 more
doaj   +1 more source

Miscanthus giganteus Biolistic Transformation Using the Visible RUBY Red Marker Gene to Monitor Transformation Efficiency

GCB Bioenergy, Volume 18, Issue 8, August 2026.
Genetic improvements to Miscanthus × giganteus (M×g), a high‐yielding perennial C4 bioenergy crop, are constrained by triploid sterility and clonal propagation. An efficient biolistic genetic transformation protocol for inflorescence‐derived callus using RUBY, a betalain‐based noninvasive reporter, provides opportunities for crop advancement and tools ...
Balasubramaniam Muthukumar, Lynn Doran, Benjamin Haas, Karolina Sobańska, Michelle Szul, Amit Kumar, Cameron Kern, Donald R. Ort, Stephen P. Long   +8 more
wiley   +1 more source

An Agrobacterium mediated transformation system of guava (Psidium guajava L.) with endochitinase gene [PDF]

Crop Breeding and Applied Biotechnology, 2014
Genetic transformation of guava (Psidium guajava L.) was developed for the first time using in vitro grown shoot tip explant cocultivated with Agrobacterium tumefaciens strain LBA4404 harbouring binary vector pIIHR-JBMch with endochitinase and nptII ...
Maneesh Mishra, Syed Uzma Jalil, Nimisha Sharma, Umesh Hudedamani   +3 more
doaj