Results 61 to 70 of about 12,375 (169)
Detection of the reporter and selection genes in transformed hop (Humulus lupulus L.)
Agrobacterium-mediated transformation of hop nodal explants with meristems was used for the introduction of a gus reporter gene and nptII plant selection gene into Slovenian hop cv. Aurora. Emerging hop regenerants were previously tested for the gus gene
Suzana ŠKOF, Zlata LUTHAR
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Gene Editing and Transformation Strategies for Engineering Drought‐Tolerant Legumes
ABSTRACT Legumes play a vital role in agriculture, nutrition, and the economy, but their production faces significant threats. Among these, drought and its unpredictability will be the most damaging constraint in the coming decades. Enhancing drought tolerance is essential for resilient and sustainable legume cultivation, and genetic engineering ...
Andrea Fernandez‐Gutierrez +2 more
wiley +1 more source
Tobacco leaf explants were transformed by bacteria Agrobacterium tumefaciens (A. t.) and plasmid pBIN mgfp5-ER, which has a single copy of the green fluorescent gfp gene and A. t.-pART27 2mgfp5-ER, which has two copies of the gfp gene. Both plasmids have
Niko PAVLIN, Zlata LUTHAR
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The efficacy of cry genes in different plant species is critical for the design and implementation of stewardship plans targeting resistance to polyphagous insects in multiple crops. Transgenic plants of three solanaceous species: Nicotiana tabacum L. (tobacco), Solanum aethiopicum L. (scarlet eggplant), and Solanum tuberosum L. (potato) were therefore
Jeanne M. E. Jacobs +2 more
wiley +1 more source
Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses.
José M. Alvarez, Ricardo J. Ordás
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Phytophthora cinnamomi is a major plant pathogen that affects economically important crops and natural ecosystems, posing a threat to global biodiversity.
Aayushree Kharel +3 more
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Regeneration of P5CS-transformed oil palm plantlets mediated by Agrobacterium tumefaciens
Environmental abiotic stressors particularly drought has detrimental effects upon the productivity of estate crops. Increasing the crop tolerance towards drought stress through genetic engineering is one of the strategies employed to maintain steady ...
Asmini Budiani +3 more
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Transient expression of uidA gene in grapevine protoplasts after PEG-mediated transformation
Leaf protoplasts, isolated from Vitis vinifera «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of Vitis sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which ...
Rahma Jardak +3 more
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An efficient method for transgenic callus induction from Vitis amurensis petiole.
Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in ...
Tingting Zhao +9 more
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Agrobacterium-опосередкована трансформація м’якої пшениці з використанням калюсних культур
Aims. Agrobacterium-mediated transformation of morphogenic calli of wheat and optimization of stages of its implementation. Methods. For transformation we аre use calli induced from apical meristems of 3-day seedlings. Results.
А. В. Бавол +3 more
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