Results 61 to 70 of about 12,375 (169)

Detection of the reporter and selection genes in transformed hop (Humulus lupulus L.)

open access: yesActa Agriculturae Slovenica, 2005
Agrobacterium-mediated transformation of hop nodal explants with meristems was used for the introduction of a gus reporter gene and nptII plant selection gene into Slovenian hop cv. Aurora. Emerging hop regenerants were previously tested for the gus gene
Suzana ŠKOF, Zlata LUTHAR
doaj   +1 more source

Gene Editing and Transformation Strategies for Engineering Drought‐Tolerant Legumes

open access: yesLegume Science, Volume 8, Issue 1, March 2026.
ABSTRACT Legumes play a vital role in agriculture, nutrition, and the economy, but their production faces significant threats. Among these, drought and its unpredictability will be the most damaging constraint in the coming decades. Enhancing drought tolerance is essential for resilient and sustainable legume cultivation, and genetic engineering ...
Andrea Fernandez‐Gutierrez   +2 more
wiley   +1 more source

The impact of plasmid on regeneration and expression efficiencies of gfp gene in tobacco (Nicotiana tabacum L.)

open access: yesActa Agriculturae Slovenica, 2014
Tobacco leaf explants were transformed by bacteria Agrobacterium tumefaciens (A. t.) and plasmid pBIN mgfp5-ER, which has a single copy of the green fluorescent gfp gene and A. t.-pART27 2mgfp5-ER, which has two copies of the gfp gene. Both plasmids have
Niko PAVLIN, Zlata LUTHAR
doaj   +1 more source

The Efficacy of a cry1Ac9 Gene Conferring Insect Resistance Is Influenced by the Target Plant Species

open access: yesNew Zealand Journal of Crop and Horticultural Science, Volume 54, Issue 1, March 2026.
The efficacy of cry genes in different plant species is critical for the design and implementation of stewardship plans targeting resistance to polyphagous insects in multiple crops. Transgenic plants of three solanaceous species: Nicotiana tabacum L. (tobacco), Solanum aethiopicum L. (scarlet eggplant), and Solanum tuberosum L. (potato) were therefore
Jeanne M. E. Jacobs   +2 more
wiley   +1 more source

Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection

open access: yesThe Scientific World Journal, 2013
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses.
José M. Alvarez, Ricardo J. Ordás
doaj   +1 more source

Target Selection, Homokaryotic Isolation, and Screening Methods for Gene Editing in the Destructive Global Pathogen, Phytophthora cinnamomi

open access: yesInternational Journal of Plant Biology
Phytophthora cinnamomi is a major plant pathogen that affects economically important crops and natural ecosystems, posing a threat to global biodiversity.
Aayushree Kharel   +3 more
doaj   +1 more source

Regeneration of P5CS-transformed oil palm plantlets mediated by Agrobacterium tumefaciens

open access: yesMenara Perkebunan, 2019
Environmental abiotic stressors particularly drought has detrimental effects upon the productivity of estate crops. Increasing the crop tolerance towards drought stress through genetic engineering is one of the strategies employed to maintain steady ...
Asmini Budiani   +3 more
doaj   +1 more source

Transient expression of uidA gene in grapevine protoplasts after PEG-mediated transformation

open access: yesOENO One, 2002
Leaf protoplasts, isolated from Vitis vinifera «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of Vitis sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which ...
Rahma Jardak   +3 more
doaj   +1 more source

An efficient method for transgenic callus induction from Vitis amurensis petiole.

open access: yesPLoS ONE, 2017
Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in ...
Tingting Zhao   +9 more
doaj   +1 more source

Agrobacterium-опосередкована трансформація м’якої пшениці з використанням калюсних культур

open access: yesФактори експериментальної еволюції організмів, 2014
Aims. Agrobacterium-mediated transformation of morphogenic calli of wheat and optimization of stages of its implementation. Methods. For transformation we аre use calli induced from apical meristems of 3-day seedlings. Results.
А. В. Бавол   +3 more
doaj  

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