Results 81 to 90 of about 4,031 (171)

The impact of plasmid on regeneration and expression efficiencies of gfp gene in tobacco (Nicotiana tabacum L.)

Acta Agriculturae Slovenica, 2014
Tobacco leaf explants were transformed by bacteria Agrobacterium tumefaciens (A. t.) and plasmid pBIN mgfp5-ER, which has a single copy of the green fluorescent gfp gene and A. t.-pART27 2mgfp5-ER, which has two copies of the gfp gene. Both plasmids have
Niko PAVLIN, Zlata LUTHAR
doaj   +1 more source

Silencing of 35S-NPTII transgene and endogenous transposable elements and other loci is affected in the mms19 mutant.

, 2015
(A) The effect of mms19 on the silencing of RD29A-LUC and 35S-NPTII transgenes. Each genotype harboring the RD29A-LUC and 35S-NPTII transgenes was grown on MS medium for 14 days followed by cold treatment for 2 days at 4 °C.
Yong-Feng Han (751209), Lin Li (28817), She Chen (167430), Huan-Wei Huang (512944), Xin-Jian He (167435), Tao Cai (19539)   +5 more
core   +1 more source

Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection

The Scientific World Journal, 2013
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses.
José M. Alvarez, Ricardo J. Ordás
doaj   +1 more source

Post-transcriptional silencing of a neomycin phosphotransferase II transgene correlates with the accumulation of unproductive RNAs and with increased cytosine methylation of 3' flanking regions

, 1997
The transgenic tobacco plant GVCHS(320)-1 harbours several T-DNAs with the neomycin phosphotransferase II (nptII)-encoding chimeric gene under control of the cauliflower mosaic virus 35S promoter (CaMV 35S).
Depicker, Anna, Van Houdt, Helena, Van Montagu, Marc, Ingelbrecht, Ivan   +3 more
core   +1 more source

Both sense and antisense RNAs are targets for the sense transgene-induced posttranscriptional silencing mechanism

, 2000
Two stable transgenic tobacco lines were obtained as segregants from a primary transformant. Plants homozygous for a T-DNA inverted repeat locus (HOlo1) showed posttranscriptional gene silencing (PTGS) of the neomycin phosphotransferase II (nptII ...
Depicker, Anna, Van Houdt, Helena, Van Montagu, Marc   +2 more
core   +1 more source

Target Selection, Homokaryotic Isolation, and Screening Methods for Gene Editing in the Destructive Global Pathogen, Phytophthora cinnamomi

International Journal of Plant Biology
Phytophthora cinnamomi is a major plant pathogen that affects economically important crops and natural ecosystems, posing a threat to global biodiversity.
Aayushree Kharel, Mark Ziemann, Jim Rookes, David M. Cahill   +3 more
doaj   +1 more source

Regeneration of P5CS-transformed oil palm plantlets mediated by Agrobacterium tumefaciens

Menara Perkebunan, 2019
Environmental abiotic stressors particularly drought has detrimental effects upon the productivity of estate crops. Increasing the crop tolerance towards drought stress through genetic engineering is one of the strategies employed to maintain steady ...
Asmini Budiani, Imam Bagus Nugroho , Hayati Minarsih, Imron Riyadi   +3 more
doaj   +1 more source

Transient expression of uidA gene in grapevine protoplasts after PEG-mediated transformation

OENO One, 2002
Leaf protoplasts, isolated from Vitis vinifera «Sakasly» and «Muscat d’Alexandrie» and protoplasts from embryogenic tissue of Vitis sp. «Seyval blanc» were incubated in the presence of PEG in a transformation solution containing the plasmid pBI426 which ...
Rahma Jardak, Ahmed Mliki, Abdelwahed Ghorbel, Götz M. Reustle   +3 more
doaj   +1 more source

An efficient method for transgenic callus induction from Vitis amurensis petiole.

PLoS ONE, 2017
Transformation is the main platform for genetic improvement and gene function studies in plants. However, the established somatic embryo transformation system for grapevines is time-consuming and has low efficiency, which limits its utilization in ...
Tingting Zhao, Zemin Wang, Lingye Su, Xiaoming Sun, Jun Cheng, Langlang Zhang, Sospeter Karanja Karungo, Yuepeng Han, Shaohua Li, Haiping Xin   +9 more
doaj   +1 more source

Regulation of alpha-amylase promoter by gibberellic acid and abscisic acid in barley protoplasts transformed by electroporation

, 1990
Transient gene expression was studied in isolated protoplasts of barley (Hordeum vulgare L. cv Himalaya) transformed by electroporation. Two plasmid constructions were used, both of which contained the gene coding for neomycin phosphotransferase II ...
Teeri, Teemu, Salmenkallio, Marjatta, Hannus, Riitta, Kauppinen, Veli   +3 more
core   +1 more source