Results 91 to 100 of about 4,031 (171)

Agrobacterium-опосередкована трансформація м’якої пшениці з використанням калюсних культур

Фактори експериментальної еволюції організмів, 2014
Aims. Agrobacterium-mediated transformation of morphogenic calli of wheat and optimization of stages of its implementation. Methods. For transformation we аre use calli induced from apical meristems of 3-day seedlings. Results.
А. В. Бавол, О. В. Дубровна, О. М. Гончарук, С. С. Воронова   +3 more
doaj  

Stable RK2-Derived Cloning Vectors for the Analysis of Gene Expression and Gene Function in Gram-Negative Bacteria

Molecular Plant-Microbe Interactions, 2001
The construction of several stable RK2-derived cloning vectors for the analysis of gene expression and function in gram-negative bacteria is reported.
Bruno Dombrecht, Jos Vanderleyden, Jan Michiels   +2 more
doaj   +1 more source

Construction of nptII–mtsfGFP fusion gene construct by overlap extension PCR [PDF]

In plant transformation, the Agrobacterium-mediated system (AMT) is widely used due to deliver transgenes into multi-layered plant cells without causing damage.
Cheng, Yang Yan
core  

Cloning and sequence analysis of truncated T-DNA inserts from Nicotiana tabacum

, 1990
Transgenic plants produced by Agrobacterium-mediated transformation usually have one or a few stable and intact T-DNA insertions. However, in a significant number of the transformants Southern blot analysis has revealed the occurrence of aberrant T-DNA ...
Gielen, Jan, Gheysen, Godelieve, Depicker, Anna, Herman, Lieve, Breyne, Peter, Van Montagu, Marc   +5 more
core   +1 more source

Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts

, 1995
Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation.
Mannonen, Leena, Salmenkallio-Marttila, Marjatta, Teeri, Teemu, Kurten, Ulrika, Kauppinen, Veli, Aspegren, Kristian, Ritala, Anneli, Åkerman, Satu   +7 more
core   +1 more source

Молекулярно-генетический анализ мозаичного характера проявления маркерного гена NPTII у трансгенных растений табака

, 2008
Подтверждено наличие не менее двух инсерций Т-ДНК в геном растений линии Nu 21. Исследован характер взаимодействия аллелей гена nptII у гибридов 1 поколения от скрещивания трансгенных растений табака линий Nu 21/5 и Nu 21/6 между собой и с растениями ...
Логинова, Д.Б., Дейнеко, Е.В.   +1 more
core  

Efficacy of an intron-containing kanamycin resistance gene as a selectable marker in plant transformation

, 2001
In this project we have analysed the use of an intron-containing neomycin phosphotransferase II - nptII - gene. The advantage of this construct is that only eukaryotic organisms will be able to process this gene.
Libiakova, G., Palmgren, G., Jørgensen, B., Ulvskov, P., Johansen, E.   +4 more
core   +1 more source

Отримання трансгенних рослин арахісу (Arachis hypogaea L.) з генами білків-стимуляторів імунної відповіді проти туберкульозу AG85, ESAT6

Фактори експериментальної еволюції організмів, 2015
Aims. The production of peanut cultivars with new properties is necessary.The purpose of this work was to develop a method of transgenesis Ag85, ESAT6 genes in peanut genome by Agrobacterium-mediated transformation. Methods.
С. М. Ніфантова, І. К. Комарницький, Ю. В. Симоненко, М. В. Кучук   +3 more
doaj  

Effective agrobacterium-mediated transformation of chicory (Cichorium intybus L.) with Mycobacterium tuberculosis antigene ESAT6 [PDF]

, 2011
Определены условия трансформации цикория Cichorium intybus L. геном антигена ESAT6 из Mycobacterium tuberculosis, которые обеспечивают наибольшую частоту регенерации зеленых растений с трансформированной ДНК. При культивировании семядолей в течение 3 сут
Банникова, М.А., Шаховский, А.М., Кваско, О.Ю., Василенко, М.Ю., Матвеева, Н.А., Кучук, Н.В.   +5 more
core   +2 more sources

Genetic characterization of cpti and nptii genes in the hybrids of transgenic and non-transgenic apple

Bangladesh Journal of Botany
To investigate the inheritance and expression of exogenous cowpea trypsin inhibitor (CpTI) and neomycin phosphotransferase II (nptII) genes in the progenies of transgenic apples (Malus domestica Borkh.), hybrid (F1) fruits and seeds were obtained using transgenic Gala and non-transgenic Fuji for cross and reciprocal cross experiments.
Ruijin Zhou, Ruiquan Dong, Xiaoxin Shi, Guoqiang Du   +3 more
openaire   +1 more source