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Фактори експериментальної еволюції організмів, 2015 Aims. The effectiveness of (Triticum aestivum L.) transformation in planta using strain AGLO harboring plasmid pBi2E with dsRNA-suppressor (double sequence RNA-suppressor) of prolinedehydrogenase gene and selective neomycin phosphotransferase II gene ...
С. С. Воронова +2 more
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The developmental stability of five successive generations of transgenic tobacco plants
, 2014 Изучены морфометрические признаки пяти поколений растений табака, трансформированных с использованием обезоруженного штамма Agrobacterium tumefaciens A699, несущего плазмиду CNL с селективным геном nptII, кодирующим белок неомицинфосфотрансферазу.Вивчено
Копытина, Т.В. +3 more
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TRANSGENIC CALLUS CULTURE ESTABLISHMENT, A TOOL FOR METABOLIC ENGINEERING OF Rhodiola rosea L.
Acta Scientiarum Polonorum: Hortorum Cultus, 2014 Agrobacterium tumefaciens EHA101 (pTd33) strain carrying uidA (GUS) reporter gene was used in model experiments on roseroot callus transformation. The T-DNA of pTd33 binary vector plasmid harbors nptII gene conferring resistance to kanamycin, and a uidA
Iman Mirmazloum +4 more
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The Bulletin of Irkutsk State University. Series Biology. Ecology, 2018 V. V. Pavlichenko +2 more
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Gene, 2012 Production of marker-free genetically modified (GM) plants is one of the major challenges of molecular fruit breeding. Employing clean vector technologies, allowing the removal of undesired DNA sequences from GM plants, this goal can be achieved. The present study describes the establishment of a clean vector system in apple Malus×domestica Borkh ...
Katja Herzog +3 more
semanticscholar +4 more sources
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Safety Assessment of the Neomycin Phosphotransferase II (NPTII) Protein
Nature Biotechnology, 1993Two approaches were used to assess the safety of the NPTII protein for human consumption using purified E. coli produced NPTII protein that was shown to be chemically and functionally equivalent to the NPTII protein produced in genetically engineered cotton seed, potato tubers and tomato fruit.
R L Fuchs, R M Leimgruber
exaly +4 more sources
Russian Journal of Genetics, 2012
Differences in the frequency of occurrence of plants with the mosaic phenotype between the Nu5 and Nu6 lines of transgenic tobacco (Nicotiana tabacum) plants remained irrespective of the allelic state of the nptII marker gene. Transition of the nptII gene from the hemizygous state (T3) to the homozygous state (T4) was accompanied by an increase in the ...
E V Deineko
exaly +4 more sources
Differences in the frequency of occurrence of plants with the mosaic phenotype between the Nu5 and Nu6 lines of transgenic tobacco (Nicotiana tabacum) plants remained irrespective of the allelic state of the nptII marker gene. Transition of the nptII gene from the hemizygous state (T3) to the homozygous state (T4) was accompanied by an increase in the ...
E V Deineko
exaly +4 more sources
Modified binary plant transformation vectors with the wild-type gene encoding NPTII
Gene, 1992The defective gene encoding neomycin phosphotransferase (NPTII) present in the binary plasmid vector, pBin19, was replaced with the wild-type (wt) gene. Plasmid vectors analogous to pBin19, pBI121 and pBI101 were constructed carrying the gene encoding the wt NPTII enzyme activity.
Lawrence E Pelcher +2 more
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