Results 121 to 130 of about 4,031 (171)
Some of the next articles are maybe not open access.
Molecular Genetics and Genomics, 1998
We have developed a novel system for the sensitive detection of nptII genes (kanamycin resistance determinants) including those present in transgenic plant genomes. The assay is based on the recombinational repair of an nptII gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter.
W Wackernagel
exaly +4 more sources
We have developed a novel system for the sensitive detection of nptII genes (kanamycin resistance determinants) including those present in transgenic plant genomes. The assay is based on the recombinational repair of an nptII gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter.
W Wackernagel
exaly +4 more sources
Mosaic expression pattern of the nptII gene in transgenic tobacco plants Nu 21
Russian Journal of Genetics, 2007Mosaic expression pattern of the nptII gene in transgenic tobacco Nu 21 leaf somatic cells was demonstrated. Inheritance of this phenotype (in T1-T4 and F1 backcrosses) was revealed. Three plant groups were distinguished, with low frequency of variegation manifestation (0-21.8%), with the high frequency of mosaic progeny (63.1 to 100%), and the ...
E V Deineko, T V Marenkova, V K Shumny
exaly +4 more sources
Hybridological analysis of inheritance of mosaic nptII gene expression in transgenic tobacco plants
Russian Journal of Genetics, 2016On the basis of the results of hybridological analysis, it was established that significant differences in the stability of manifestation of the nptII gene expression are observed between the Nu5 and Nu6 lines obtained from the same initial Nu21 transformant (in spite of the identical genetic environment).
T. V. Marenkova, E. Deineko
semanticscholar +3 more sources
Expression of the NPTII-sequence in cauliflower after injection of agrobacteria into seeds
Journal of Plant Physiology, 1992Summary A simple procedure for gene transfer into cauliflower by macroinjection is described. Seeds of Brassica oleracea var. botrytis were injected with different Agrobacterium tumefaciens strains. The kanamycin-resistance coding NPTII region of engineered Ti-plasmid could be integrated into the plant genome. Expression as well as incorporation of
Klaus Eimert
exaly +2 more sources
In Vitro Cellular & Developmental Biology - Plant, 2018
Soybean transformation is limited by the lack of multiple efficient selectable marker systems. Biolistic transformation of somatic proliferative embryogenic cultures, one of the commonly used soybean transformation methods, relies largely on hygromycin phosphotransferase II (hptII) selection.
A. Itaya, Su-Qin Zheng, D. Simmonds
semanticscholar +2 more sources
Soybean transformation is limited by the lack of multiple efficient selectable marker systems. Biolistic transformation of somatic proliferative embryogenic cultures, one of the commonly used soybean transformation methods, relies largely on hygromycin phosphotransferase II (hptII) selection.
A. Itaya, Su-Qin Zheng, D. Simmonds
semanticscholar +2 more sources
Transfer and expression of nptII and bar genes in cucumber (Cucumis satavus L.)
In Vitro Cellular & Developmental Biology - Plant, 2007Abstract The generation of transgenic Cucumis sativus cv. Greenlong plants resistant to phosphinothricin (PPT) was obtained using Agrobacterium tumefaciens-mediated gene transfer. The protocol relied on the regeneration of shoots from cotyledon explants.
G. Vengadesan +4 more
semanticscholar +2 more sources
Bacterial Species Specificity in proU Osmoinducibility and nptII and lacZ Expression
Journal of molecular microbiology and biotechnology, 2005Reporter gene-based transcriptional fusions are increasingly being used to address questions in microbial ecology, with constitutively expressed fusions enabling microbe tracking and inducible fusions reporting the presence of environmental signals. To more readily apply this technology to a variety of bacterial species, we examined species specificity
Catherine A. Wright, G. Beattie
semanticscholar +3 more sources
Cloning and Stem Cells, 2008
Both enhanced green fluorescence protein (EGFP) and neomycin phosphotransferase type II enzyme (NPTII) are widely used in transgenic studies, but their side effects have not been extensively investigated. In this study, we evaluated the expression profiles of the two marker genes and the relationship between their expression and ...
Yan Liu, Xiuzhu Sun, Huiting Cui
exaly +3 more sources
Both enhanced green fluorescence protein (EGFP) and neomycin phosphotransferase type II enzyme (NPTII) are widely used in transgenic studies, but their side effects have not been extensively investigated. In this study, we evaluated the expression profiles of the two marker genes and the relationship between their expression and ...
Yan Liu, Xiuzhu Sun, Huiting Cui
exaly +3 more sources
A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants
Russian Journal of Genetics, 2006A comparative analysis of variability of heterologous gene expression was conducted in original (T0) transgenic tobacco plants, differing with respect to the copy number of uidA gene (a single and two copies as a direct duplication) within the T-DNA insertion, and among progenies obtained by their self-pollination (T1).
T. V. Marenkova, E. Deineko
semanticscholar +3 more sources