Results 141 to 150 of about 4,031 (171)

??????????????????????-???????????????????????? ???????????? ???????????????????? ?????????????????? ???????????????????? ???????????????????? ???????? NPTII ?? ?????????????????????? ???????????????? ????????????

2021
Presence of no less then two T-DNA insertions into line Nu 21 plant genome was confirmed. Interaction character between nptII gene alleles was analyzed in Nu 21/5, Nu 21/6 lines 1 generation crossing hybrids and in Nu 21/5, SR1 (wild-type line) and Nu 21/6, SR1 1 generation crossing hybrids. Plasmid pC27-nuclS T-DNA part sequence was determined. It was
openaire   +1 more source

UV Inducible Transient Expression of Chimeric CHS-NPTII in Parsley Proto — Plasts

1988
Light is a very important effector for differentiation in plant development. One of the most intensivly studied regulatory effects of (UV B)-light on specific metabolic pathways is the induction of flavonoid biosynthesis (Kreuzaler et al., 1983). Flavonoids are abundant in higher plants and function as flower pigments and UV protective compounds ...
S. Lipphardt, F. Kreuzaler, J. Dangl, J. Schell   +3 more
openaire   +1 more source

Nucleosome positioning in the NPTII neomycin phosphotransferase gene on a yeast plasmid in the repressed and active states

Molecular Biology, 2008
A yeast plasmid was constructed to contain a hybrid GAL-CYC promoter, the NPTII neomycin phosphotransferase gene, and the FRT sequence between them. The CYC part of the GAL-CYC promoter harbored four upstream activating sequences (UASs) and two close TATA boxes.
G. I. Kiryanov, L. V. Isayeva, L. N. Kintsurashvili, M. G. Zakharova   +3 more
openaire   +1 more source

Modified in planta transformation procedure for Agrobacterium tumefaciens mediated NptII in brinjal (Solanum melongina)

Progressive Horticulture, 2016
In the present study, optimization of in planta genetic transformation of Solanum melongena L. var. CVK (Covai Vari Kathari) was attempted using seed as an explant and Agrobacterium tumefaciens strain LBA4404 containing the gene construct 35S: nptII: RCI2A in the binary vector pBI121.
J.A. Jenifer, S. Girija, Anjana Hari, R. Sudha, E.P. Venkatasalam   +4 more
openaire   +1 more source

Acquisition of a collection of Lycopersicon pennellii (Corr. D'Arcy) transgenic plants with uidA and nptII marker genes

The Journal of Horticultural Science and Biotechnology, 1999
SummaryThis paper describes an efficient protocol for in vitro regeneration and genetic transformation from leaf explants of L. pennellii (Corr. D'Arcy). Shoot organogenesis was achieved on media with different combinations of indole-acetic acid (IAA) and kinetin (K). Regeneration ratios ranged from 50 to 90%.
C. Gisbert, I. Arrillaga, L. A. Roig, V. Moreno   +3 more
openaire   +1 more source

Agrobacterium-mediated transformation efficiency of carrot embryogenic callus using nptII and hpt selective genes

Russian Agricultural Sciences, 2015
Experiments were carried out to compare the efficiency of embryogenic carrot calli transformation using selective genes nptII and hpt. Our data showed that it is more preferable to use kanamycin concentration 100 mg/L and hygromycin 20 mg/L for the selection of transgenic shoots.
T. N. Sidorova, D. N. Miroshnichenko, S. V. Dolgov   +2 more
openaire   +1 more source

Detection of nptII Gene and 35SCaMV Promoter in Tomatoes (Solanum lycopersicum L.)

2013
The detection of nptII (kanamycin resistance) as a transgenic marker gene and 35SCaMV as promoter in tomatoes has been carried out. DNA from tomatoes samples was isolated using PureLinkTM plant total DNA purification kit. The purity of DNA samples was estimated using UV-Vis spectrophotometry at 260 nm and 280 nm.
Suratman, A., Ughude, J. O., ., Sismindari   +2 more
openaire   +1 more source

Efficient seed-specifically regulated autoexcision of marker gene (nptII) with inducible expression of interest gene in transgenic Nicotiana tabacum

, 2016
Shiva Hamzeh, M. Motallebi, M. Zamani
semanticscholar   +1 more source

[Nucleosome positioning within neomycinphosphotransferase gene (NPTII) on yeast plasmid in repressed and active state].

Molekuliarnaia biologiia, 2009
Yeast recombinant plasmid containing FRT-sequence flanked by hybrid GAL-CYC promoter and NPTII gene was developed. GAL-CYC promoter contains four UAS sequences and two closely associated TATA-boxes in CYC part. This construct provides galactose-inducible synthesis of neomycinphosphotransferase from NPTII gene, and, thus, resistance of transformed cells
G I, Kir'ianov, L V, Isaeva, L N, Kintsurashvili, M G, Zakharova   +3 more
openaire   +1 more source

Small RNAs were involved in homozygous state-associated silencing of a marker gene (Neomycin phosphotransferase II: nptII) in transgenic tomato plants.

Plant physiology and biochemistry : PPB, 2013
Lei Deng, Yu Pan, Xu-Qing Chen, Guoping Chen, Zong-Li Hu   +4 more
semanticscholar   +1 more source