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Detection of nptII Gene and 35SCaMV Promoter in Tomatoes (Solanum lycopersicum L.)
2013The detection of nptII (kanamycin resistance) as a transgenic marker gene and 35SCaMV as promoter in tomatoes has been carried out. DNA from tomatoes samples was isolated using PureLinkTM plant total DNA purification kit. The purity of DNA samples was estimated using UV-Vis spectrophotometry at 260 nm and 280 nm.
Suratman, A. +2 more
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Molekuliarnaia biologiia, 2009
Yeast recombinant plasmid containing FRT-sequence flanked by hybrid GAL-CYC promoter and NPTII gene was developed. GAL-CYC promoter contains four UAS sequences and two closely associated TATA-boxes in CYC part. This construct provides galactose-inducible synthesis of neomycinphosphotransferase from NPTII gene, and, thus, resistance of transformed cells
G I, Kir'ianov +3 more
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Yeast recombinant plasmid containing FRT-sequence flanked by hybrid GAL-CYC promoter and NPTII gene was developed. GAL-CYC promoter contains four UAS sequences and two closely associated TATA-boxes in CYC part. This construct provides galactose-inducible synthesis of neomycinphosphotransferase from NPTII gene, and, thus, resistance of transformed cells
G I, Kir'ianov +3 more
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NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants
2003I S, Curtis, J B, Power, M R, Davey
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