Results 131 to 140 of about 12,375 (169)
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Mosaic expression pattern of the nptII gene in transgenic tobacco plants Nu 21
Russian Journal of Genetics, 2007Mosaic expression pattern of the nptII gene in transgenic tobacco Nu 21 leaf somatic cells was demonstrated. Inheritance of this phenotype (in T1-T4 and F1 backcrosses) was revealed. Three plant groups were distinguished, with low frequency of variegation manifestation (0-21.8%), with the high frequency of mosaic progeny (63.1 to 100%), and the ...
Shumny V K, Marenkova T V, Deineko E V
exaly +3 more sources
Modified binary plant transformation vectors with the wild-type gene encoding NPTII
Gene, 1992The defective gene encoding neomycin phosphotransferase (NPTII) present in the binary plasmid vector, pBin19, was replaced with the wild-type (wt) gene. Plasmid vectors analogous to pBin19, pBI121 and pBI101 were constructed carrying the gene encoding the wt NPTII enzyme activity.
Lawrence E Pelcher +2 more
exaly +3 more sources
Russian Journal of Genetics, 2012
Differences in the frequency of occurrence of plants with the mosaic phenotype between the Nu5 and Nu6 lines of transgenic tobacco (Nicotiana tabacum) plants remained irrespective of the allelic state of the nptII marker gene. Transition of the nptII gene from the hemizygous state (T3) to the homozygous state (T4) was accompanied by an increase in the ...
Deineko E V, E V Deineko
exaly +3 more sources
Differences in the frequency of occurrence of plants with the mosaic phenotype between the Nu5 and Nu6 lines of transgenic tobacco (Nicotiana tabacum) plants remained irrespective of the allelic state of the nptII marker gene. Transition of the nptII gene from the hemizygous state (T3) to the homozygous state (T4) was accompanied by an increase in the ...
Deineko E V, E V Deineko
exaly +3 more sources
Molecular Genetics and Genomics, 1998
We have developed a novel system for the sensitive detection of nptII genes (kanamycin resistance determinants) including those present in transgenic plant genomes. The assay is based on the recombinational repair of an nptII gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter.
W Wackernagel, Wackernagel W
exaly +3 more sources
We have developed a novel system for the sensitive detection of nptII genes (kanamycin resistance determinants) including those present in transgenic plant genomes. The assay is based on the recombinational repair of an nptII gene with an internal 10-bp deletion located on a plasmid downstream of a bacterial promoter.
W Wackernagel, Wackernagel W
exaly +3 more sources
Cloning and Stem Cells, 2008
Both enhanced green fluorescence protein (EGFP) and neomycin phosphotransferase type II enzyme (NPTII) are widely used in transgenic studies, but their side effects have not been extensively investigated. In this study, we evaluated the expression profiles of the two marker genes and the relationship between their expression and ...
Yan Liu, Xiuzhu Sun, Yunping Dai
exaly +3 more sources
Both enhanced green fluorescence protein (EGFP) and neomycin phosphotransferase type II enzyme (NPTII) are widely used in transgenic studies, but their side effects have not been extensively investigated. In this study, we evaluated the expression profiles of the two marker genes and the relationship between their expression and ...
Yan Liu, Xiuzhu Sun, Yunping Dai
exaly +3 more sources
Induction of Expression Instability of the nptII Gene in Transgenic Tobacco Plants
Russian Journal of Plant Physiology, 2002A comparative analysis of the neomycin phosphotransferase (nptII) gene expression was performed in two groups of transformed tobacco plants, one of which included plants with direct and inverted tandem uidA gene repeats in the T-DNA insertion. This insertion of inverted repeats was shown to reduce the level of stable nptII gene expression to 20%, as ...
T. V. Novoselya, E. V. Deineko
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Agricultural Research Journal, 2022
In the present study, selection media was optimized, for in vitro regenerated sugarcane transformants using nptII gene as negative selectable marker, that kills non-transformed cells and ensures proliferation and growth of only transformed cells. To identify a suitable agent and its optimal dose, the embryogenic calli of sugarcane were bombarded with ...
P K Malhotra +3 more
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In the present study, selection media was optimized, for in vitro regenerated sugarcane transformants using nptII gene as negative selectable marker, that kills non-transformed cells and ensures proliferation and growth of only transformed cells. To identify a suitable agent and its optimal dose, the embryogenic calli of sugarcane were bombarded with ...
P K Malhotra +3 more
openaire +1 more source
Hybridological analysis of inheritance of mosaic nptII gene expression in transgenic tobacco plants
Russian Journal of Genetics, 2016On the basis of the results of hybridological analysis, it was established that significant differences in the stability of manifestation of the nptII gene expression are observed between the Nu5 and Nu6 lines obtained from the same initial Nu21 transformant (in spite of the identical genetic environment).
T V, Marenkova, E V, Deineko
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A change in the stability of marker nptII and uidA gene expression in transgenic tobacco plants
Russian Journal of Genetics, 2006A comparative analysis of variability of heterologous gene expression was conducted in original (T0) transgenic tobacco plants, differing with respect to the copy number of uidA gene (a single and two copies as a direct duplication) within the T-DNA insertion, and among progenies obtained by their self-pollination (T1).
T V, Marenkova, E V, Deĭneko
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Worldview, 1980
Throughout 1980 the moral buffer zone between domestic and world politics has been widening dangerously. Because our communications media have been preoccupied with presidential and senatorial election campaigns—and because those campaigns have been almost totally innocent of any rational deliberation over foreign policy— the American people have ...
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Throughout 1980 the moral buffer zone between domestic and world politics has been widening dangerously. Because our communications media have been preoccupied with presidential and senatorial election campaigns—and because those campaigns have been almost totally innocent of any rational deliberation over foreign policy— the American people have ...
openaire +1 more source