Results 31 to 40 of about 12,375 (169)

The stability of the Arabidopsis transcriptome in transgenic plants expressing the marker genes nptII and uidA [PDF]

The Plant Journal, 2005
Summary The ATH1 Arabidopsis GeneChip from Affymetrix was used to search for transcriptome changes in Arabidopsis associated with the strong expression of transgenes regulated by constitutive promoters. The insertion and expression of the commonly used marker genes, uidA and
Souad, El Ouakfaoui, Brian, Miki
openaire   +2 more sources

Cloning of the nptII gene of Escherichia coli and construction of a recombinant strain harboring functional recA and nptII antibiotic resistance

Genetics and Molecular Research, 2011
In an attempt to clone the ORF of the nptII gene of Escherichia coli K12 (ATCC 10798), two degenerate primers were designed based on the nptII sequence of its Tn5 transposon. The nptII ORF was placed under the control of the E. coli hybrid trc promoter, in the pKK388-1 vector, transformed into E.
openaire   +2 more sources

Transgenic selection and underlying mechanisms in apicomplexan parasites

The FEBS Journal, EarlyView.
Transgenic selection markers have driven genetic engineering in apicomplexans, enabling precise, iterative experiments. This review discusses mechanistic details of drug selection markers, strategies for marker recycling, and practical considerations for several clinically relevant parasites.
Swaroop Peddiraju, Rakhee Lohia, Nishith Gupta   +2 more
wiley   +1 more source

Agrobacterium tumefaciens-mediated transformation of Jatropha curcas L. with a polyhydroxyalkanoate gene (phaC)

Indonesian Journal of Biotechnology, 2018
Polyhydroxybutyrate is a component of bioplastics that is synthesized under the control of enzymes encoded by pha multigenes. The genes are naturally present in Ralstonia eutropha. However, the production of bioplastics in bacteria is inefficient because
Chesara Novatiano, Adi Pancoro, Erly Marwani   +2 more
doaj   +1 more source

Gene Editing of Nicotiana benthamiana Architecture for Space‐Efficient Production of Recombinant Proteins in Closed Environments

Plant Biotechnology Journal, EarlyView.
ABSTRACT Indoor vertical farming (VF) offers practical advantages for the cultivation of plant protein bio‐factories including plant uniformity, product consistency, water/nutrient recycling and production cycles on a year‐round basis. Much progress has been achieved toward the development of innovative systems for artificial lighting, automated ...
Béatrice Giroux, Kristina LeBreux, Louis Feyzeau, Marie‐Claire Goulet, Charles Goulet, Dominique Michaud   +5 more
wiley   +1 more source

Unexpected Suppression of Tomato Fruit Cuticle Formation by Overexpression of a Key Cutin Biosynthetic Enzyme

Plant Biotechnology Journal, EarlyView.
ABSTRACT Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience.
Gulab Chand Arya, Ekram Wassel, Vibha Mishra, Ekaterina Manasherova, Ruth E. Stark, Hagai Cohen   +5 more
wiley   +1 more source

A downstream box fusion allows stable accumulation of a bacterial cellulase in Chlamydomonas reinhardtii chloroplasts

Biotechnology for Biofuels, 2018
Background We investigated strategies to improve foreign protein accumulation in the chloroplasts of the model algae Chlamydomonas reinhardtii and tested the outcome in both standard culture conditions as well as one pertinent to algal biofuel production.
Lubna V. Richter, Huijun Yang, Mohammad Yazdani, Maureen R. Hanson, Beth A. Ahner   +4 more
doaj   +1 more source

NbRD21 protease controls receptor kinase homeostasis in Nicotiana benthamiana

New Phytologist, Volume 251, Issue 5, Page 2741-2752, September 2026.
Proteomic analysis revealed that receptor kinases (red) accumulate higher in rd21 knockout Nicotiana benthamiana plants. Summary RD21‐like proteases are papain‐like cysteine proteases with a C‐terminal granulin domain that are abundant and ubiquitous in angiosperms and have often been implicated in immunity.
Alice Godson, Lauren Eddie, Mariana Schuster, Kaijie Zheng, Reka Toth, Yuge Li, Tianrun Li, Jie Huang, Farnusch Kaschani, Philippe V. Jutras, Jiorgos Kourelis, Markus Kaiser, Tolga Bozkurt, Renier A. L. Van der Hoorn   +13 more
wiley   +1 more source

Most of the Specific Genomic Regions of Agrobacterium fabrum Shape Phenolics Content of Medicago truncatula Roots

Environmental Microbiology Reports, Volume 18, Issue 4, August 2026.
Agrobacterium fabrum harbour seven specific‐gene regions, whose annotation indicates a close connection with plant. To evaluate their involvement in plant‐bacteria interaction, deletion mutant strains of each specific region were inoculated onto Medicago truncatula roots. Root phenolics content was influenced by them.
Rosa Padilla, Ludovic Vial, Florence Dechèvre, Thi Huyen Thu Nguyen, Deniz Sarigol, Vincent Gaillard, Guillaume Meiffren, Gilles Comte, Xavier Nesme, Céline Lavire, Isabelle Kerzaon   +10 more
wiley   +1 more source

Отримання трансгенних рослин картоплі (Solanum tuberosum L.), що містять антизмістовну послідовність гена проліндегідрогенази

Фактори експериментальної еволюції організмів, 2018
Мета. Метою роботи було отримання трансгенних рослин картоплі районованих в Україні сортів, у яких проходить експресія дволанцюгового РНК-супресора проліндегідрогенази, що призводить до зменшення деградації проліну і підвищення його концентрації в ...
O. O. Ovcharenko, V. A. Rudas, N. L. Shcherbak, M. V. Kuchuk   +3 more
doaj   +1 more source