Results 61 to 70 of about 3,097 (169)

Use of direct somatic organogenesis for Agrobacterium-mediated transformation of onion

Acta Biologica Slovenica, 2003
An Agrobacterium tumefaciens mediated DNA delivery is being developed for onion (Allium cepa L.) using organogenic structures formed on the ovaries as target tissue. An indirect transformation procedure using A.
Manja-Tina Bastar, Zlata Luthar, Borut Bohanec   +2 more
doaj   +1 more source

Transient expression of the β-glucuronidase gene in Cannabis sativa varieties

Plant Signaling & Behavior, 2020
In plant biology, transient expression analysis plays a vital role to provide a fast method to study the gene of interest. In this study, we report a rapid and efficient method for transient expression in Cannabis sativa seedlings using Agrobacterium ...
Aleksei Sorokin, Narendra Singh Yadav, Daniel Gaudet, Igor Kovalchuk   +3 more
doaj   +1 more source

Field selection of elite events of East African highland bananas expressing elevated levels of pro‐vitamin A

Plant Biotechnology Journal, Volume 23, Issue 9, Page 3713-3726, September 2025.
Summary Biofortification of staple crops is a sustainable strategy to deliver essential micronutrients to impoverished populations in developing countries. Banana is a highly valued crop consumed by over 75% of Ugandans. However, the starchy green cooking bananas have very low levels of pro‐vitamin A (PVA) and heavy dietary reliance on them has been ...
Stephen Buah, Jimmy Moses Tindamanyire, Priver Namanya, Jerome Kubiriba, Geoffrey Arinaitwe, Jean‐Yves Paul, Robert Harding, James Dale, Wilberforce Tushemereirwe   +8 more
wiley   +1 more source

Patch‐Clamp‐ and 15N Isotope Tracer‐Based Techniques Reveal the Transport Characteristics of SlGAT1 and the Metabolic Flow of Exogenous GABA Under Salt Stress

Plant, Cell &Environment, Volume 48, Issue 9, Page 6820-6834, September 2025.
ABSTRACT γ‐Aminobutyric acid (GABA) plays an important role in plant growth, development, and stress resistance. Exogenous GABA works by increasing the content of endogenous GABA; therefore, GABA transport from the apoplast to the cytosol is critical. In this study, two GABA transporter genes, SlGAT1 and SlGAT2 were identified in tomato.
Ding Yuan, Xiao‐Lei Wu, Xiang‐Qun Jiang, Zu‐Wen Lan, Li‐Ran Zhao, Yun‐Duan Wang, Hong‐Bo Gao   +6 more
wiley   +1 more source

STEMIN transcription factor drives selective chromatin remodeling for gene activation within a relaxed chromatin during reprogramming in the moss Physcomitrium patens

The Plant Journal, Volume 123, Issue 3, August 2025.
SUMMARY Land plants exhibit remarkable cellular plasticity, readily reprogramming differentiated cells into stem cells in response to internal and external stimuli. While chromatin remodeling is crucial for cellular reprogramming, its interplay with gene expression during reprogramming into stem cells remains elusive.
Ruan Morné de Villiers, Gergo Palfalvi, Akinori Kanai, Yutaka Suzuki, Mitsuyasu Hasebe, Masaki Ishikawa   +5 more
wiley   +1 more source

β‐Glucuronidase‐Expressing Lactobacillus reuteri Triggers Irinotecan Enterotoxicity Through Depleting the Regenerative Epithelial Stem/Progenitor Pool

Advanced Science, Volume 12, Issue 26, July 10, 2025.
XLP mitigated CPT11 mucositis by suppressing GUS‐expressing microbes, notably L. reuteri, and diminishing bacterial GUS activity, consequently reducing SN38 accumulation to protect the intestinal epithelium. This preservation of the mucosal stem cell niche enabled rapid regeneration of secretory lineages such as mucin‐producing goblet cells, which ...
Bei Yue, Ruiyang Gao, Ling Zhao, Donghui Liu, Cheng Lv, Ziyi Wang, Fangbin Ai, Beibei Zhang, Zhilun Yu, Xiaolong Geng, Hao Wang, Kang Wang, Kaixian Chen, Chenghai Liu, Zhengtao Wang, Wei Dou   +15 more
wiley   +1 more source

Efficient and versatile rapeseed transformation for new breeding technologies

The Plant Journal, Volume 123, Issue 1, July 2025.
SUMMARY Many gene functions are widely studied and understood in Arabidopsis; however, the lack of efficient transformation systems often limits the application and verification of this knowledge in crop plants. Brassica napus L., a member of the Brassicaceae family, is usually transformed by Agrobacterium‐mediated hypocotyl transformation, but not all
Kea Ille, Siegbert Melzer
wiley   +1 more source

The Arabidopsis PM19L1 Protein Functions as a Regulator of Germination Under Osmotic Stress

Plant Direct, Volume 9, Issue 5, May 2025.
ABSTRACT How plants perceive and respond to water availability, especially during the critical stages of seed formation and germination, is key to their survival. During development, ripening, and germination, seeds undergo large changes in water content, down to around 10% during maturation and up to 90% again within 24 h of germination.
Ross D. Alexander, Pablo Castillejo‐Pons, Nina Melzer, Omar Alsaif, Vivien I. Strotmann, Yvonne Stahl, Madeleine Seale, Peter C. Morris   +7 more
wiley   +1 more source

Eficiência de transformação genética de citrange 'carrizo' com duas construções gênicas Genetic transformation efficiency of 'carrizo' citrange with two gene constructions

Revista Brasileira de Fruticultura, 2011
Transformação genética é considerada uma importante ferramenta auxiliar no melhoramento genético de plantas cítricas. Entretanto, a eficiência de transformação pode variar em função de diversos fatores, incluindo a própria construção gênica utilizada ...
Luzia Yuriko Miyata, Francisco de Assis Alves Mourão Filho, João Alexio Scarpare Filho, Flávia Zambon, Meire Menezes Bassan, Beatriz Madalena Januzzi Mendes, Ricardo Harakava   +6 more
doaj  

xTAG Luminex multiplex assay for rapid screening of verocytotoxin-producing Escherichia coli strains

Romanian Journal of Laboratory Medicine, 2018
The O26 verocytotoxin-producing Escherichia coli (VTEC)-associated outbreak of hemolytic uremic syndrome (HUS) cases in Romania during 2016 showed the need to improve the current methodology of non-O157 VTEC detection and surveillance.
Oprea Mihaela, Usein Codruța-Romanița
doaj   +1 more source