Results 31 to 40 of about 4,575 (159)

Rapid detection of coliforms in drinking water of Arak city using multiplex PCR method in comparison with the standard method of culture (Most Probably Number)

Asian Pacific Journal of Tropical Biomedicine, 2014
Objective: To analyse molecular detection of coliforms and shorten the time of PCR. Methods: Rapid detection of coliforms by amplification of lacZ and uidA genes in a multiplex PCR reaction was designed and performed in comparison with most probably ...
Dehghan fatemeh, Zolfaghari Mohammad Reza, Arjomandzadegan Mohammad, Kalantari Salomeh, Ahmari Gholam Reza, Sarmadian Hossein, Sadrnia Maryam, Ahmadi Azam, Shojapoor Mana, Najarian Negin, Kasravi Alii Reza, Falahat Saeed   +11 more
doaj   +1 more source

Improved efficiency of uidA gene transfer in stone pine (Pinus pinea) cotyledons using a modified binary vector

, 1999
This paper describes the construction of a binary vector with the uidA-int gene under the control of the promoter and first intron from the maize (Zea mays L.) ubiquitin 1 (Ubi1) gene, aiming at high expression levels of the uidA gene in Pinus pinea L ...
Jaime M Humara, Francisco Parra, Ricardo J Ordás, M Soledad Marín   +3 more
core   +1 more source

Shiga Toxin Profiles of Poultry-origin Escherichia coli Isolates with Low Levels of Antimicrobial Resistance to the Clinical and Laboratory Standard Institute-recommended Antibiotics for Surveillance in Human Beings

Indian Journal of Public Health
Background: Escherichia coli are the reservoirs of antimicrobial resistance (AMR) genes. Shiga toxin-producing E. coli of animal origin can cause severe life-threatening infections in man.
Maansi Soodan, Kavita Rana, Ashok Kumar Panda, Krishanender Dinesh, Rajesh Kumar, Rajesh Kumar Vaid, Varun Sankhyan, Sidharath Dev Thakur   +7 more
doaj   +1 more source

Biomass‐derived hydrochars as eco‐friendly adsorbents for wastewater treatment applications

Environmental Progress &Sustainable Energy, Volume 45, Issue 4, July/August 2026.
Abstract Emerging organic pollutants (EOPs), such as diethyl phthalate (DEP), bisphenol A (BPA), and methylene blue (MB), are only partially removed in conventional wastewater treatment plants. This study assesses hydrochars produced by hydrothermal carbonization (HTC) of spruce bark (SB), vine shoots (VSs), and wheat straw (WSs) for removing three ...
Emanuel Gheorghita Armanu, Sara Ribeirinho‐Soares, Christian Eberlein, Hermann J. Heipieper, Irina Volf, Olga C. Nunes, Olívia Salomé G. P. Soares   +6 more
wiley   +1 more source

Stable Agrobacterium-Mediated Transformation of Maritime Pine Based on Kanamycin Selection

The Scientific World Journal, 2013
An efficient transformation protocol based on kanamycin selection was developed for Agrobacterium-mediated transformation of maritime pine embryonal masses.
José M. Alvarez, Ricardo J. Ordás
doaj   +1 more source

Efficient genetic transformation of Lotus corniculatus L. using a direct shoot regeneration protocol, stepwise hygromycin B selection, and a super-binary Agrobacterium tumefaciens vector [PDF]

Archives of Biological Sciences, 2007
Cotyledons from 6-day-old Lotus corniculatus cv. Bokor seedlings, transversally cut into two halves, were capa­ble of regenerating buds without intervening callus formation.
Nikolić Radomirka, Mitić Nevena, Ninković Slavica, Nešković Mirjana   +3 more
doaj   +1 more source

Emerging Dissemination of blaCTX‐M‐65 in Bovine E. coli in Spain Associated With IncHI2 Plasmids

MicrobiologyOpen, Volume 15, Issue 3, June 2026.
The resistome in ESBL/AmpC‐producing E. coli in livestock was diverse and dominated by plasmid‐borne genes Detection of host‐independent dissemination of AMR determinants mediated by shared plasmid types First report in Spanish cattle of blaCTX‐M‐65 on IncHI2 plasmids, an under‐reported ESBL in Europe Identification of potentially zoonotic clones in ...
Medelin Ocejo, Beatriz Oporto, Ana Hurtado   +2 more
wiley   +1 more source

The promoter from SlREO, a highly-expressed, root-specific Solanum lycopersicum gene, directs expression to cortex of mature roots [PDF]

, 2008
Root-specific promoters are valuable tools for targeting transgene expression, but many of those already described have limitations to their general applicability.
Wright, Carole, Thompson, Andrew J., Andrews, John, Taylor, Ian B., Manning, Kenneth, Jones, Matthew O.   +5 more
core   +1 more source

An Escherichia coli Phosphotransferase System Modulates Methylglyoxal Resistance by Regulating Intracellular Potassium

Molecular Microbiology, Volume 125, Issue 6, Page 461-474, June 2026.
Escherichia coli can survive methylglyoxal stress by modulating phosphorylation of a regulatory phosphotransferase system, which, in turn, regulates the activity of a potassium/proton antiporter. The constitutive potassium importer Trk also contributes to intracellular potassium levels.
Sara Alexander, Mark Goulian
wiley   +1 more source

Optimization of coffee (Coffea arabica) transformation parameters using uidA and hpt genes: effect of osmotic pre-treatment, helium pressure and target distance

, 2016
The aim of this work was to optimize the biolistic delivery parameters that affect the DNA delivery and stable expression of marker genes into coffee tissues (Coffea arabica. L. cvs. Caturra and Catuaí).
Espinoza Esquivel, Ana Mercedes, Arrieta Espinoza, Griselda, Gatica Arias, Andrés Mauricio   +2 more
core   +1 more source