Results 101 to 110 of about 883,268 (297)
FEBS Letters, EarlyView.An unexpected alternative interaction site for ethyl viologen was identified in formate dehydrogenase 1 from Methylorubrum extorquens. Combined mutagenesis, kinetic analysis, and docking revealed that aromatic residues near an iron–sulfur cluster enable flavin mononucleotide‐independent electron transfer, offering a framework for engineering improved ...
Eleni G. Poloniataki, Yong Hwan Kim
wiley +1 more source
Transformation of phosphiothrici resistance gene(bar)in Undaria pinnatifida gametophytes [PDF]
, 2012 裙带菜(Undaria pinnatifida)配子体经2.5 L气升式光生物反应器快速营养增殖,通过基因枪法将携带草丁膦抗性基因(bar)的载体转入扩增后的配子体,经草丁膦筛选,获得抗性克隆。提取抗性克隆配子体基因组总DNA,经PCR和PCR Southern检测,结果显示60%的抗性克隆可检出bar基因,表明bar基因是裙带菜配子体基因工程有效的筛选标记 ...
任宝永 +4 more
core
FEBS Letters, EarlyView.The physical dimensions and shape of bacterial cells define the surface area available to acquire nutrients and the volume available for synthesizing proteins and DNA. Here, we use computational systems biology to decode the importance of cell geometry as a major determinant of prokaryotic phenotype, including growth rate and metabolic efficiency. This
Ross P. Carlson +6 more
wiley +1 more source
Expression and Inheritance of bar Gene in Petunia hybrida Transformed with Agrobacterium [PDF]
Journal of Plant Biotechnology, 2003 This experiment was carried out to confirm the stability of bar gene introduced into petunia plant through Agrobacerium-mediated transformation. Twenty-five transgenic plants T plants, back cross (BC) populations to wild type and Fplants between different T plants were prepared, and polymerase chain reaction(PCR), PCR-Southern blot analysis, and field ...
openaire +1 more source
From mice to humans—divergent strategies for intestinal homeostasis and regeneration
FEBS Letters, EarlyView.Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa +2 more
wiley +1 more source
Mean field analysis of a spatial stochastic model of a gene regulatory network [PDF]
, 2015 A gene regulatory network may be defined as a collection of DNA segments which interact with each other indirectly through their RNA and protein products.
Murray, P. J. +3 more
core +1 more source
BMC Cancer, 2007 Background This study investigated the effects of hyperoxic treatment on growth, angiogenesis, apoptosis, general morphology and gene expression in DMBA-induced rat mammary tumors.
Steen Vidar M +5 more
doaj +1 more source
FEBS Letters, EarlyView.Inositol pyrophosphates are energy‐rich signaling molecules that perform critical functions in cells. Three different families of phosphatases hydrolyze the β phosphate of the inositol pyrophosphate molecules: two have narrow specificities and one is promiscuous.
Ronda J. Rolfes
wiley +1 more source
The bialaphos resistance gene (bar) plays a role in both self-defense and bialaphos biosynthesis in Streptomyces hydroscopicus [PDF]
, 1988 We inactivated the bialaphos (BA) resistance gene (bar) of a BA producer, Streptomyces hygroscopicus, by the gene replacement technique. The resulting BA-sensitive mutant (Bar-) was able to produce little BA but considerable amount of an intermediate ...
Takano, E.; id_orcid +8 more
core
STEM: a tool for the analysis of short time series gene expression data-0 [PDF]
, 2011 Copyright information:Taken from "STEM: a tool for the analysis of short time series gene expression data"BMC Bioinformatics 2006;7():191-191.Published online 5 Apr 2006PMCID:PMC1456994.Copyright © 2006 Ernst and Bar-Joseph; licensee BioMed Central Ltd ...
Jason Ernst (24617) +1 more
core +1 more source