Results 111 to 120 of about 31,885,483 (388)

High-throughput sequencing of core STR loci for forensic genetic investigations using the Roche Genome Sequencer FLX platform

BioTechniques, 2011
The analysis and profiling of short tandem repeat (STR) loci is routinely used in forensic genetics. Current methods to investigate STR loci, including PCR-based standard fragment analyses and capillary electrophoresis, only provide amplicon lengths that
Sarah L. Fordyce, Maria C. Ávila-Arcos, Eszter Rockenbauer, Claus Børsting, Rune Frank-Hansen, Frederik Torp Petersen, Eske Willerslev, Anders J. Hansen, Niels Morling, M. Thomas. P. Gilbert   +9 more
doaj   +1 more source

Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutations.

PLoS ONE, 2020
The programmable sequence specificity of CRISPR has found uses in gene editing and diagnostics. This manuscript describes an additional application of CRISPR through a family of novel DNA enrichment technologies.
Jennifer L Steele, Richard C Stevens, Oscar A Cabrera, Gary J Bassill, Sabrina M Cramer, Felipe Guzman, Anthony P Shuber   +6 more
doaj   +1 more source

The structure of kinetoplast DNA. 1. The mini-circles of Crithidia lucilae are heterogeneous in base sequence.

European Journal of Biochemistry, 1976
We have analysed limit digests of mini-circles from kinetoplast DNA of Crithidia luciliae by gel electrophoresis. Endonucleases HapII and AluI cut the circles into at least 37 and 21 fragments, respectively, and leave no circles intact. In both cases the
C. M. Kleisen, Piet Borst, P. Weijers
semanticscholar   +1 more source

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

FEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley, Daniel H. Shain, Eric A. Klein   +2 more
wiley   +1 more source

The Error and Repair Catastrophes: A Two-Dimensional Phase Diagram in the Quasispecies Model

, 2003
This paper develops a two gene, single fitness peak model for determining the equilibrium distribution of genotypes in a unicellular population which is capable of genetic damage repair.
A. Giraud, A. Sasaki, C.O. Wilke, C.O. Wilke, C.O. Wilke, C.O. Wilke, D. Alves, E. Tannenbaum, E. van Nimwegen, F. Taddei, I. Matic, J. LeClerc, J. Swetina, K. Negishi, M. Eigen, M. Eigen, M. Kolar, M. Nilsson, M. Nilsson, O. Tenaillon, P. Schuster, P. Tarazona, P.D. Sniegowski, P.D. Sniegowski, P.R.A. Campos, R. Pastor-Satorras, S. Altmeyer, S. Crotty, S. Franz, S. Galluccio   +29 more
core   +1 more source

A repetitive sequence assembler based on next-generation sequencing

Genetics and Molecular Research, 2016
Repetitive sequences of variable length are common in almost all eukaryotic genomes, and most of them are presumed to have important biomedical functions and can cause genomic instability. Next-generation sequencing (NGS) technologies provide the possibility of identifying capturing these repetitive sequences directly from the NGS data.
Y Wang, Y Tu, Xinwu Chen, Shuaibin Lian, Ling-Ling Wang   +4 more
openaire   +3 more sources

DNA base sequence changes and sequence specificity of bromodeoxyuridine-induced mutations in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America, 1988
By using a shuttle vector system developed in our laboratory, we have carried out studies on the molecular mechanism by which 5-bromodeoxyuridine (BrdUrd) induces mutations in mammalian cells.
R. Davidson, P. Broeker, C. Ashman
semanticscholar   +1 more source

Elucidation of interface interactions between a dehydratase domain and an acyl carrier protein in cremimycin polyketide synthase

FEBS Letters, EarlyView.
In modular polyketide synthases, the dehydratase (DH) domain catalyzes the dehydration reaction of the β‐hydroxyacyl unit attached to the cognate acyl carrier protein (ACP) domain. However, it is unclear how DH interacts with ACP during the reaction. In this study, we identified DH–ACP interface residues, providing the first detailed insights into DH ...
Kaede Kotagiri, Haruka Tachibana, Daisuke Kawasaki, Taichi Chisuga, Toma Kashima, Shinya Fushinobu, Fumitaka Kudo, Tadashi Eguchi, Akimasa Miyanaga   +8 more
wiley   +1 more source

Low Base-Substitution Mutation Rate but High Rate of Slippage Mutations in the Sequence Repeat-Rich Genome of Dictyostelium discoideum

G3: Genes, Genomes, Genetics, 2020
We describe the rate and spectrum of spontaneous mutations for the social amoeba Dictyostelium discoideum, a key model organism in molecular, cellular, evolutionary and developmental biology. Whole-genome sequencing of 37 mutation accumulation lines of D.
Sibel Kucukyildirim, Megan Behringer, Way Sung, Debra A. Brock, Thomas G. Doak, Hatice Mergen, David C. Queller, Joan E. Strassmann, Michael Lynch   +8 more
doaj   +1 more source

Binary Random Sequences Obtained From Decimal Sequences [PDF]

arXiv, 2008
This paper presents a twist to the generation of binary random sequences by starting with decimal sequences. Rather than representing the prime reciprocal sequence directly in base 2, we first right the prime reciprocal in base 10 and then convert it into the binary form. The autocorrelation and cross-correlation properties of these binary random (BRD)
arxiv