Results 51 to 60 of about 842,010 (246)

PuLSE: Quality control and quantification of peptide sequences explored by phage display libraries. [PDF]

PLoS ONE, 2018
The design of highly diverse phage display libraries is based on assumption that DNA bases are incorporated at similar rates within the randomized sequence.
Steven Shave, Stefan Mann, Joanna Koszela, Alastair Kerr, Manfred Auer   +4 more
doaj   +1 more source

Purification tags markedly affect self‐aggregation of CPEB3

FEBS Letters, EarlyView.
Although recombinant proteins are used to study protein aggregation in vitro, uncleaved tags can interfere with accurate interpretation. Our findings demonstrate that His₆‐GFP and His₁₂ tags significantly affect liquid droplet and amyloid fibril formation in the intrinsically disordered region (IDR) of mouse cytoplasmic polyadenylation element‐binding ...
Harunobu Saito, Yujin Lee, Motoharu Ueno, Naotaka Sekiyama, Masatomo So, Ayako Furukawa, Kenji Sugase   +6 more
wiley   +1 more source

Entering the post-genomic era of malaria research

Bulletin of the World Health Organization, 2000
The sequencing of the genome of Plasmodium falciparum promises to revolutionize the way in which malaria research will be carried out. Beyond simple gene discovery, the genome sequence will facilitate the comprehensive determination of the parasite?s ...
Horrocks Paul, Bowman Sharen, Kyes Susan, Waters Andrew P., Craig Alister   +4 more
doaj  

Improving the specificity of nucleic acid detection with endonuclease-actuated degradation

Communications Biology, 2022
A new method, SENTINEL, improves the specificity of nucleic acid target sequence identification via a combination of isothermal amplification and sequence-specific DNA endonucleases. The method can discriminate single base-pair differences and takes less
Roger S. Zou, Momcilo Gavrilov, Yang Liu, Dominique Rasoloson, Madison Conte, Justin Hardick, Leo Shen, Siqi Chen, Andrew Pekosz, Geraldine Seydoux, Yukari C. Manabe, Taekjip Ha   +11 more
doaj   +1 more source

Circulating histones as clinical biomarkers in critically ill conditions

FEBS Letters, EarlyView.
Circulating histones are emerging as promising biomarkers in critical illness due to their diagnostic, prognostic, and therapeutic potential. Detection methods such as ELISA and mass spectrometry provide reliable approaches for quantifying histone levels in plasma samples.
José Luis García‐Gimenez, Juan Carlos Ruiz‐Rodríguez, Ricard Ferrer, Raquel Durá, Antonio Artigas, Iván Bajaña, David Bolado López de Andujar, Irene Cánovas‐Cervera, Adrián Ceccato, Luis Chiscano‐Camón, Elena Climent‐Martinez, Georgia García Fernández, Gemma Goma, Verónica Monforte, Beatriz Quevedo‐Sánchez, Adolf Ruiz‐Sanmartín, Antonio Sierra‐Rivera, Nieves Carbonell Monleón   +17 more
wiley   +1 more source

بررسی بیوانفورماتیک ارتباط اندازه‌ی ژنوم با خصوصیات ژنتیکی و رفتاری باکتری‌های بیماری‌زا

مجله دانشکده پزشکی اصفهان, 2014
مقدمه: با افزایش تعداد باکتری‌هایی که توالی‌یابی می‌شوند، نیاز به بهره‌برداری از این داده‌ها نیز بیشتر می‌شود. استفاده از این اطلاعات ژنومی از طریق به کارگیری هم‌زمان علوم بیوانفورماتیک و باکتری‌شناسی امکان‌ پذیر می‌باشد.
Mohammad Reza Pourmand, Zahra Pakbaz, Abbas Rahimi-Foroushani, Solmaz Ohadian-Moghadam   +3 more
doaj  

Diagnostic value of 3D-Cube T2 sequence combined with plain CT scan of the skull base for cerebrospinal fluid rhinorrhea

Di-san junyi daxue xuebao, 2019
Objective To evaluate the diagnostic value of magnetic resonance 3D-Cube T2 sequence, plain CT scan of the skull base and their combination for cerebrospinal fluid (CSF) rhinorrhea and assess the performance of these 2 modalities combined in improving ...
GAN Hui, ZHANG Song, JIANG Liling, GE Xiaodong, ZOU Liguang   +4 more
doaj   +1 more source

Single‐cell insights into the role of T cells in B‐cell malignancies

FEBS Letters, EarlyView.
Single‐cell technologies have transformed our understanding of T cell–tumor cell interactions in B‐cell malignancies, revealing new T‐cell subsets, functional states, and immune evasion mechanisms. This Review synthesizes these findings, highlighting the roles of T cells in pathogenesis, progression, and therapy response, and underscoring their ...
Laura Llaó‐Cid
wiley   +1 more source

RY-Coding and Non-Homogeneous Models Can Ameliorate the Maximum-Likelihood Inferences from Nucleotide Sequence Data with Parallel Compositional Heterogeneity

Evolutionary Bioinformatics, 2012
In phylogenetic analyses of nucleotide sequences, ‘homogeneous’ substitution models, which assume the stationarity of base composition across a tree, are widely used, albeit individual sequences may bear distinctive base frequencies.
Sohta A. Ishikawa, Yuji Inagaki, Tetsuo Hashimoto   +2 more
doaj   +1 more source

Contribution of nonconsensus base pairs within ArsR binding sequences toward ArsR-DNA binding and arsenic-mediated transcriptional induction

Journal of Biological Engineering, 2019
Background A transcriptional reporter is the key component in bacterial biosensors which are employed to monitor the induction or repression of a reporter gene corresponding to environmental change.
Xingjuan Chen, Xin Jiang, Cuijuan Tie, Jinnon Yoo, Yan Wang, Meiying Xu, Guoping Sun, Jun Guo, Xianqiang Li   +8 more
doaj   +1 more source