Results 81 to 90 of about 316,488 (268)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Study on Load-Bearing Characteristics and Engineering Applications for Cement–Soil Pipe Pile

Buildings
The cement–soil pipe pile is a novel blend of cement and soil, designed to enhance load-bearing capabilities while cutting down on the need for cement. Its tubular construction is key to its strength.
Chong Zhou, Xiangzhuo Zheng, Sifeng Zhang, Chao Li, Yaohui Yang, Jianyong Han   +5 more
doaj   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Three-Dimensional Discrete Element Analysis of Bearing Characteristics of Concrete–Cored Sand–Gravel Pile Composite Foundation under Cyclic Dynamic Load

Buildings
Concrete-cored sand–gravel piles are a kind of composite pile formed by wrapping a concrete-cored pile with a sand–gravel shell, which has the advantages of both a rigid pile and bulk-material pile.
Binhui Ma, Wenbiao Tang, Tian Lan, Xu Deng, Bicheng Du   +4 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Model Test Study on the Bearing Characteristics of Pile Groups with Different Pile Spacing

工程科学与技术
为探究桩间距对群桩竖向承载性状及群桩效应的影响机制，自主研发了“多功能桩基静动力模型试验系统”，开展了饱和砂土中单桩和3d、4d和6d（d为桩径）桩间距群桩的模型试验，系统揭示了桩间距对桩侧摩阻力、桩端阻力、承台底土反力、群桩效应的影响规律和机理。研究结果表明：单桩和群桩的荷载-沉降曲线均呈缓变型。群桩的极限承载力和效应系数随桩间距的增大呈线性增长。桩间距不改变桩侧阻力的分布模式。随着荷载的增大，不同桩间距的群桩桩侧阻力沿深度方向先增大后减小，均呈“单峰”状。桩间距越大，桩侧阻力越大 ...
DENG Yuchen, CHEN Chen, YANG Qi, LENG Wuming, DING Rongfeng   +4 more
doaj  

Review of research progress on the theory and bearing characteristics of post-grouting for prestressed concrete pipe pile ends and sides

河南理工大学学报. 自然科学版
Significance Post-grouting of pipe piles is an effective technique for improving the physical and mechanical properties of the pile-soil interface， playing a significant role in enhancing the frictional resistance of pipe piles and reducing settlement of
HOU Zhenkun, CHEN Xuanlin, TANG Mengxiong, LING Zao, DU Xidong, XU Feng, HE Shaoyang, LIANG Shihua   +7 more
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Finite element simulation study on vertical bearing characteristics of single pile with ram-compacted bearing sphere. [PDF]

PLoS One, 2023
Bai L, Gong S, Xu L, Bai X, Huang Z.
europepmc   +1 more source