Results 41 to 50 of about 4,937 (196)

A fully automatable enzymatic method for DNA extraction from plant tissues [PDF]

, 2005
Background DNA extraction from plant tissues, unlike DNA isolation from mammalian tissues, remains difficult due to the presence of a rigid cell wall around the plant cells.
Jean-François Manen, Olga Sinitsyna, Lorène Aeschbach, Alexander V Markov, Arkady Sinitsyn   +4 more
core   +2 more sources

Purification and some properties of .BETA.-xylosidase from Trichoderma viride.

Agricultural and Biological Chemistry, 1984
β-Xylosidase was purified 25 fold from a culture filtrate by ammonium sulfate fractionation, DEAE-Sephadex chromatography, column electrophoresis, gel filtration on Biogel P-100, and isoelectric focusing. The purified β-xylosidase was found to be homogeneous on SDS (sodium dodecyl sulfate) polyacrylamide gel electrophoresis and on disc electrophoresis.
Masaru MATSUO, Tsuneo YASUI
openaire   +2 more sources

Plant diversity is not a consistent predictor of soil extracellular enzyme activity in field or experimental grasslands

Ecosphere, Volume 17, Issue 5, May 2026.
Abstract Soil extracellular enzymes are produced and excreted by soil microbial organisms. They catalyze many of the biochemical reactions that support crucial ecosystem functions like decomposition, organic matter mineralization, nutrient cycling, and C sequestration.
Jennifer K. Bell, Nicholas A. Barber, Kendall K. Beals, Camille Traylor, Samantha Berk, Isaac Bailey‐Marren, Mary‐Claire Glasenhardt, Andrew L. Hipp, Katharine Hogan, Holly P. Jones, Alec Juliano, Emma Leavens, Cooper Rosin, Zheng Yao, Paul Zedler, Meghan G. Midgley   +15 more
wiley   +1 more source

Active protein aggregates induced by terminally attached self-assembling peptide ELK16 in Escherichia coli

Microbial Cell Factories, 2011
Background In recent years, it has been gradually realized that bacterial inclusion bodies (IBs) could be biologically active. In particular, several proteins including green fluorescent protein, β-galactosidase, β-lactamase, alkaline phosphatase, D ...
Zhou Bihong, Xing Lei, Wu Wei, Lin Zhanglin   +3 more
doaj   +1 more source

Novel GH115 xylan α‐1,2‐glucuronidases with distinct preferences for xylan‐derived oligomers and polymers

Protein Science, Volume 35, Issue 5, May 2026.
Abstract Natural polymers are promising sustainable materials for diverse applications. Xylans, major components of hemicellulose, exhibit origin‐dependent substitution patterns that determine their physicochemical properties. Targeted enzymatic modification of these substituents offers a mild and precise approach for tailoring the polymer's ...
Savvina Leontakianakou, Simone Balzer Le, Anders Sundin, Andrius Jasilionis, Giang‐Son Nguyen, Anna Nordborg, Lalitha D. Gottumukkala, Carl Grey, Anna Sofia Lewin, Eva Nordberg Karlsson   +9 more
wiley   +1 more source

Moderate Phosphorus Addition to Field-Grown Bananas Enhanced Soil Microbial Enzyme Activities but Had Negligible Impacts on Bacterial, Fungal, and Nematode Diversity

Applied Microbiology
On commercial banana (Musa spp.) plantations, soils are often supplemented with phosphorus (P) fertiliser to optimise production. Such additions may influence the diversity and function of soil microbial communities, which play important roles in P ...
Anna-Belle C. Clarke, Hazel R. Lapis-Gaza, Stuart Irvine-Brown, Rebecca Lyons, Jiarui Sun, Anthony B. Pattison, Paul G. Dennis   +6 more
doaj   +1 more source

Small surfactant-like peptides can drive soluble proteins into active aggregates

Microbial Cell Factories, 2012
Background Inactive protein inclusion bodies occur commonly in Escherichia coli (E. coli) cells expressing heterologous proteins. Previously several independent groups have found that active protein aggregates or pseudo inclusion bodies can be induced by
Zhou Bihong, Xing Lei, Wu Wei, Zhang Xian-En, Lin Zhanglin   +4 more
doaj   +1 more source

A novel xylan degrading β-D-xylosidase: purification and biochemical characterization [PDF]

, 2012
Aspergillus ochraceus, a thermotolerant fungus isolated in Brazil from decomposing materials, produced an extracellular b-xylosidase that was purified using DEAE-cellulose ion exchange chromatography, Sephadex G-100 and Biogel P-60 gel filtration.
A Knob, A Sunna, A Wiseman, ACS Rizzatti, BC Saha, BC Saha, BC Saha, BJ Davis, BL Zamost, DS Ruzene, FA Ximenes, FF Zanoelo, GH Miller, H Lineweaver, Héctor F. Terenzi, I Kimura, JD Fontana, JHA Betini, José A. Teixeira, João A. Jorge, K Iwashita, K Li, K Poutanen, K-E Eriksson, KKY Wong, KKY Wong, L Viikari, LE Rasmussem, M Dubois, M Guerfali, M Kurakabe, M Michelin, M Wakiyama, Maria de Lourdes T. M. Polizeli, MC Herrmann, Michele Michelin, MLTM Polizeli, MLTM Polizeli, N Kulkarni, OH Lowry, P Biely, P Biely, QJ Yan, RM Peralta, Simone C. Peixoto-Nogueira, Tony M. Silva, UK Laemmli, V Lenartovicz, VC Sandrim   +48 more
core   +1 more source

Purification and some properties of .BETA.-xylosidase from Emericella nidulans.

Agricultural and Biological Chemistry, 1984
β-Xylosidase was purified 662 fold from a culture filtrate by ammonium sulfate fractionation, gel filtration on Biogel P-100, DEAE-Sephadex chromatography, and gel filtration on Sephadex G-200. With isoelectric focusing, the purified β-xylosidase found to be homogeneous on SDS (sodium dodecyl sulfate) polyacrylamide gel electrophoresis.
Masaru MATSUO, TSUNEO YASUI
openaire   +2 more sources

Necromass chemistry drives the functional diversity of the necrobiome, resulting in microbe–organic matter feedbacks

Functional Ecology, Volume 40, Issue 5, Page 1339-1351, May 2026.
Read the free Plain Language Summary for this article on the Journal blog. Abstract In temperate European forests, soil fungal communities, dominated by saprotrophic and ectomycorrhizal (ECM) species, represent almost 25% of soil organic carbon (C) in the soil.
Elsa Hilaire, Laure Fauchery, Michaël Danger, Aurélie Cébron, Bernhard Zeller, Marc Buée   +5 more
wiley   +1 more source