Results 181 to 190 of about 20,671 (222)

Effects of bilirubin and its photoisomers on direct bilirubin measurement using bilirubin oxidase

Annals of Clinical Biochemistry, 2000
We examined the reactivity of human serum albumin-bound bilirubin and its photoisomers as substrates for a direct bilirubin assay using bilirubin oxidase. The reduction of (EZ)-cyclobilirubin reached 100% 5 min after addition of the enzyme at any pH tested (3·5–7·4) in 0·1 mol/L phosphate buffer, whereas the reduction of (ZE)-bilirubin or (ZZ ...
S, Itoh, T, Kusaka, T, Imai, K, Isobe, S, Onishi   +4 more
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Determination of direct-bilirubin by a fluorimetric-enzymatic method based on bilirubin oxidase

Fresenius' Journal of Analytical Chemistry, 2000
A method for the determination of direct bilirubin by reaction with bilirubin oxidase (BOx) is reported. The procedure is based on the changes in fluorescence which take place during the enzymatic reaction of BOx with any of the three forms of bilirubin (free, conjugated and with albumin) when the solution is excited at 240 nm and the emission is ...
Y, Andreu, J, Galbán, S, de Marcos, J R, Castillo   +3 more
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Enhanced oxidation of bilirubin by an immobilized tri-enzyme system of glucose oxidase, bilirubin oxidase and horseradish peroxidase

Biochimica et Biophysica Acta (BBA) - General Subjects, 1989
Bilirubin oxidase was immobilized to nylon fibres. A tri-enzyme system composed of glucose oxidase, bilirubin oxidase and horseradish peroxidase was also immobilized to the fibres. Both immobilized systems were tested and it was found that the latter gave enhanced oxidation rates for bilirubin.
N J, Daka, R, Sipehia, T M, Chang
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Features and applications of bilirubin oxidases

Applied Microbiology and Biotechnology, 2012
Discovered in 1981 by Tanaka and Murao (Agric Biol Chem 45:2383-2384, 1981), bilirubin oxidase (BOD) is a sub-group of multicopper oxidases (MCOs) also utilizing four Cu(+/2+) ions. It catalyzes the oxidation of bilirubin to biliverdin, hence the classification of bilirubin oxidase, and has been primarily used in the determination of bilirubin in serum
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Conformation of bilirubin oxidase in native and denatured states

Journal of Protein Chemistry, 1994
The conformation of bilirubin oxidase (EC 1.3.3.5) from Myrothecium verrucaria was studied by circular dichroism (CD). The far-UV CD spectrum showed a single minimum at 215 nm and a maximum near 198 nm, suggesting the dominance of beta-sheets. There was another negative band at 187 nm that is absent from the spectra of model alpha-helix or beta-sheet ...
T, Samejima, C S, Wu, K, Shiboya, H, Kaji, S, Koikeda, K, Ando, J T, Yang   +6 more
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Bilirubin oxidases in bioelectrochemistry: Features and recent findings

Biosensors and Bioelectronics, 2013
Bilirubin oxidases, a sub class of the Multicopper oxidases family, were discovered in 1981 by Tanaka and Murao (Murao and Tanaka, 1981) and first used for the detection of bilirubin. Since 2001 and the pioneering work of Tsujimura, these BODs have attracted a lot of attention for the reduction of O2.
Nicolas, Mano, Lise, Edembe
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Enzymatic polymerization of dihydroquercetin using bilirubin oxidase

Biochemistry (Moscow), 2015
Dihydroquercetin (or taxifolin) is one of the most famous flavonoids and is abundant in Siberian larch (Larix sibirica). The oxidative polymerization of dihydroquercetin (DHQ) using bilirubin oxidase as a biocatalyst was investigated and some physicochemical properties of the products were studied.
M E, Khlupova, I S, Vasil'eva, G P, Shumakovich, O V, Morozova, V A, Chertkov, A K, Shestakova, A V, Kisin, A I, Yaropolov   +7 more
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Electrochemical consideration on the optimum pH of bilirubin oxidase

Analytical Biochemistry, 2007
Steady-state current-potential curves were obtained for the direct electron transfer (DET) of bilirubin oxidase (BOD) at a highly oriented pyrolytic graphite electrode, and the theoretical analysis based on nonlinear regression enabled us to determine the formal redox potential (E degrees') of BOD in a wide pH range of 2.0 to 8.5.
Kaori, Otsuka, Takayasu, Sugihara, Yoshio, Tsujino, Toshiyuki, Osakai, Eiichi, Tamiya   +4 more
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Electrochemistry of bilirubin oxidase at carbon nanotubes

Journal of Solid State Electrochemistry, 2009
In this paper, we compared the direct electron transfer and electrocatalytic properties of bilirubin oxidase (BOD) immobilized at two kinds of carbon nanotubes (CNTs), bamboo-CNTs and uniform-CNTs. X-ray diffraction and X-ray photoelectron spectroscopy results indicated that the ratio of sp2 band to sp3 band and the content of oxygen-containing groups ...
W. Zheng, H. Y. Zhao, H. M. Zhou, X. X. Xu, M. H. Ding, Y. F. Zheng   +5 more
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Quantitative effects of bilirubin photoisomers on the measurement of direct bilirubin by the enzymatic bilirubin oxidase method

Annals of Clinical Biochemistry: International Journal of Laboratory Medicine
Background Bilirubin photoisomers, generated during phototherapy or incidental light exposure, may interfere with direct bilirubin (DB) measurement using the bilirubin oxidase method. This interference is particularly relevant in neonates, who physiologically exhibit elevated levels of unconjugated bilirubin.
Nana Kawaguchi, Kosuke Koyano, Hirosuke Morita, Dk Nur Rosyiidah Apryll Czarina Pengiran Mohamad Fadly, Yuta Shinabe, Yuta Noguchi, Makoto Arioka, Yasuhiro Nakao, Miyo Ozaki, Shinji Nakamura, Sonoko Kondo, Yukihiko Konishi, Toru Kuboi, Hitoshi Okada, Saneyuki Yasuda, Susumu Itoh, Koji Murao, Takashi Kusaka   +17 more
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