Results 261 to 270 of about 8,121,841 (424)

A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

FEBS Open Bio, EarlyView.
We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei, Amir Barzegar Behrooz, Marco Cordani, Mahmoud Aghaei, Negar Azarpira, Daniel J. Klionsky, Saeid Ghavami   +6 more
wiley   +1 more source

Enhancing transporter activity in heterologous expression systems with SAHA: a 2500‐times more potent and odorless alternative to butyrate

FEBS Open Bio, EarlyView.
Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann   +4 more
wiley   +1 more source

An enzyme‐linked immunosorbent assay (ELISA)‐based activity assay for AMP‐activated protein kinase (AMPK)

FEBS Open Bio, EarlyView.
Measuring AMP‐activated protein kinase (AMPK) activity in vitro is crucial for testing AMPK activators or inhibitors with therapeutic potential. Here, we report an enzyme‐linked immunosorbent assay (ELISA)‐based AMPK activity assay with simple steps and high sensitivity, which offers a simple, robust, and cost‐effective alternative to traditional ...
Trezze P. Nguyen, Shangze Lyu, Yang Liu
wiley   +1 more source

Machine learning approaches for predicting protein-ligand binding sites from sequence data. [PDF]

Front Bioinform
Vural O, Jololian L.
europepmc   +1 more source

Proteolytic fragments of troponin C. Localization of high and low affinity Ca2+ binding sites and interactions with troponin I and troponin T

, 1978
Paul C. Leavis, Steven S. Rosenfeld, J. Gergely, Zenon Grabarek, W. Drabikowski   +4 more
openalex   +1 more source

Target mRNAs are repressed as efficiently by microRNA-binding sites in the 5′ UTR as in the 3′ UTR

Proceedings of the National Academy of Sciences of the United States of America, 2007
J. Lytle, T. Yario, J. Steitz
semanticscholar   +1 more source

Bioengineering facets of the tumor microenvironment in 3D tumor models: insights into cellular, biophysical and biochemical interactions

FEBS Open Bio, EarlyView.
The tumor microenvironment is a dynamic, multifaceted complex system of interdependent cellular, biochemical, and biophysical components. Three‐dimensional in vitro models of the tumor microenvironment enable a better understanding of these interactions and their impact on cancer progression and therapeutic resistance.
Salma T. Rafik, Deniz Bakkalci, Alexander J. MacRobert, Umber Cheema   +3 more
wiley   +1 more source

Mapping small molecule-RNA binding sites via Chem-CLIP synergized with capillary electrophoresis and nanopore sequencing. [PDF]

Nucleic Acids Res
Yang X, Wang J, Springer NA, Zanon PRA, Jia Y, Su X, Disney MD.   +6 more
europepmc   +1 more source

Identification of a tyrosine residue at a nucleotide binding site in the beta subunit of the mitochondrial ATPase with p-fluorosulfonyl[14C]-benzoyl-5'-adenosine.

, 1978
Frederick Esch, W S Allison
openalex   +1 more source

Rpn1 provides adjacent receptor sites for substrate binding and deubiquitination by the proteasome

Science, 2016
Yuan Shi, X. Chen, S. Elsasser, B. Stocks, G. Tian, Byung-Hoon Lee, Yanhong Shi, Naixia Zhang, S. A. D. de Poot, Fabian Tuebing, Shuangwu Sun, J. Vannoy, S. Tarasov, J. Engen, D. Finley, K. Walters   +15 more
semanticscholar   +1 more source