Results 41 to 50 of about 3,236,821 (283)

Black glucose-releasing silicon elastomer rings for fed-batch operation allow measurement of the oxygen transfer rate from the top and optical signals from the bottom for each well of a microtiter plate

BMC Biotechnology, 2023
Background In industrial microbial biotechnology, fed-batch processes are frequently used to avoid undesirable biological phenomena, such as substrate inhibition or overflow metabolism.
Sarah Sparviero, Laura Barth, Timm Keil, Carl Dinter, Christoph Berg, Clemens Lattermann, Jochen Büchs   +6 more
doaj   +1 more source

RAD50 missense variants differentially affect the DNA damage response and mitotic progression

FEBS Letters, EarlyView.
RAD50 incorporates into the MRN complex and initiates the DNA damage response. Furthermore, RAD50 promotes mitotic progression. RAD50 missense variants capable of forming an MRN complex supported the DNA damage response and mitotic features to different extents in complementation experiments, indicating these functions are separable and might impact ...
Hanna Redeker, Swantje Kebel, Lea Völkening, Anna Vatselia, Louisa Weinhold, Girmay Asgedom, Axel Schambach, Detlev Schindler, Thilo Dörk, Kristine Bousset   +9 more
wiley   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Pitfalls in Early Bioprocess Development Using Shake Flask Cultivations

Engineering in Life Sciences
For about 100 years, the shake flask has been established for biotechnological cultivations as one of the most important cultivation systems in early process development.
Gesa Brauneck, Dominik Engel, Luca Antonia Grebe, Maximilian Hoffmann, Philipp Georg Lichtenberg, Anne Neuß, Marcel Mann, Jorgen Barsett Magnus   +7 more
doaj   +1 more source

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2,3-butanediol production

Microbial Cell Factories, 2019
Background Production of 2,3-butanediol from renewable resources is a promising measure to decrease the consumption of fossil resources in the chemical industry.
Benedikt Heyman, Robin Lamm, Hannah Tulke, Lars Regestein, Jochen Büchs   +4 more
doaj   +1 more source

Benchmarking biopharmaceutical process development and manufacturing cost contributions to R&D

mAbs, 2020
This study aims to benchmark and analyze the process development and manufacturing costs across the biopharmaceutical drug development cycle and their contribution to overall research and development (R&D) costs.
Suzanne S. Farid, Max Baron, Christos Stamatis, Wenhao Nie, Jon Coffman   +4 more
doaj   +1 more source

Interaction and Mechanism of Probiotics with Gut Flora and Immune Regulation: A Review

Shipin gongye ke-ji, 2022
Probiotics play beneficial roles in improving the host intestinal microecological balance and improving the host health level and health state. It mainly affects the host health by regulating the intestinal microbiota and participating in the immune ...
Manyu TANG, Wanqing WANG, Jingwen QIANG, Wei HUA, Shuang WU, Yali LI, Xin ZHEN, Chungeng LI, Yanling CHENG   +8 more
doaj   +1 more source

4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase in malaria parasites and enhances atovaquone efficacy

FEBS Letters, EarlyView.
Atovaquone is an antimalarial requiring potentiation for sufficient efficacy. We pursued strategies to enhance its activity, showing that 4‐nitrobenzoate inhibits 4‐hydroxybenzoate polyprenyltransferase, decreasing ubiquinone biosynthesis. Since atovaquone competes with ubiquinol in mitochondria, 4‐nitrobenzoate facilitates its action, potentiating ...
Ignasi Bofill Verdaguer, Matheus Felipe Santos, Maurício Mazzine Filho, Gabriela Oliveira Castro, Agustín Hernández, Manoel Aparecido Peres, Alejandro Miguel Katzin, Marcell Crispim   +7 more
wiley   +1 more source

Optimization of L-lactic acid production by fermentation of Huaimi dregs

Biotechnology & Biotechnological Equipment, 2018
Utilization of waste dregs, e.g. Huaimi dregs, is an important issue. Huaimi is a traditional Chinese medicine and 20,000 tons of dregs are annually produced. This study optimized the production of L-lactic acid through solid-state fermentation of Huaimi
Jingsheng Zhang, Zhiyuan Guo, Bingchen Liu, Yue Wang, Na Yu, Qing Huo   +5 more
doaj   +1 more source

Linked dimers of the AAA+ ATPase Msp1 reveal energetic demands and mechanistic plasticity for substrate extraction from lipid bilayers

FEBS Letters, EarlyView.
Cells must clear mislocalized or faulty proteins from membranes to survive. The AAA+ ATPase Msp1 performs this task, but dissecting how its six subunits work together is challenging. We engineered linked dimers with varied numbers of functional subunits to reveal how Msp1 subunits cooperate and use energy to extract proteins from the lipid bilayer ...
Deepika Gaur, Brian Acquaviva, Baylee A. Smith, Nathan Walker, Isabella Walter, Matthew L. Wohlever   +5 more
wiley   +1 more source