Results 51 to 60 of about 508,313 (310)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Selective mRNA translation during eIF2 phosphorylation induces expression of IBTKα

, 2014
Disruption of protein folding in the endoplasmic reticulum (ER) triggers the unfolded protein response (UPR), a transcriptional and translational control network designed to restore protein homeostasis.
McClintick, Jeanette N., Anthony, Tracy G., Fusakio, Michael E., Davis, Christopher M., Willy, Jeffrey A., Baird, Thomas D., Palam, Lakshmi Reddy, Wek, Ronald C.   +7 more
core   +1 more source

Phosphatidylinositol 4‐kinase as a target of pathogens—friend or foe?

FEBS Letters, EarlyView.
This graphical summary illustrates the roles of phosphatidylinositol 4‐kinases (PI4Ks). PI4Ks regulate key cellular processes and can be hijacked by pathogens, such as viruses, bacteria and parasites, to support their intracellular replication. Their dual role as essential host enzymes and pathogen cofactors makes them promising drug targets.
Ana C. Mendes, Guilherme M. Azevedo, Amanda P. Barcellos, Diana Bahia   +3 more
wiley   +1 more source

Mechanosensitive Hair Cell-Like Cells from Embryonic and Induced Pluripotent Stem Cells

, 2018
Mechanosensitive sensory hair cells are the linchpin of our senses of hearing and balance. The inability of the mammalian inner ear to regenerate lost hair cells is the major reason for the permanence of hearing loss and certain balance disorders.
Peng, AW, Diensthuber, M, Heller, S, Ricci, AJ, Oshima, K, Shin, K   +5 more
core   +1 more source

Protein pyrophosphorylation by inositol pyrophosphates — detection, function, and regulation

FEBS Letters, EarlyView.
Protein pyrophosphorylation is an unusual signaling mechanism that was discovered two decades ago. It can be driven by inositol pyrophosphate messengers and influences various cellular processes. Herein, we summarize the research progress and challenges of this field, covering pathways found to be regulated by this posttranslational modification as ...
Sarah Lampe, Tanmay Kumar Mohanty, Rashna Bhandari, Dorothea Fiedler   +3 more
wiley   +1 more source

Essential role of p21Waf1/Cip1 in the modulation of post-traumatic hippocampal Neural Stem Cells response

Stem Cell Research & Therapy
Background Traumatic Brain Injury (TBI) represents one of the main causes of brain damage in young people and the elderly population with a very high rate of psycho-physical disability and death.
Francesco Chiani, Valentina Mastrorilli, Nicole Marchetti, Andrea Macioce, Chiara Nappi, Georgios Strimpakos, Miriam Pasquini, Alessia Gambadoro, Jonathan Isacco Battistini, Debora Cutuli, Laura Petrosini, Sara Marinelli, Raffaella Scardigli, Stefano Farioli Vecchioli   +13 more
doaj   +1 more source

Inhibitors of sterol synthesis. Submicromolar 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta, 15 alpha-diol causes a major modification of the sterol composition of CHO-K1 cells and a marked change in cell morphology.

Journal of Lipid Research, 1994
Incubation of Chinese hamster ovary cells (CHO-K1) with 14 alpha-ethyl-5 alpha-cholest-7-ene-3 beta,15 alpha-diol (0.1 microM) in lipid-deficient medium led to a major change in cellular sterol composition, which was characterized by a very marked ...
A Izumi, F.D Pinkerton, S.O Nelson, J.S Pyrek, P.J Neill, J H Smith, G.J Schroepfer, Jr   +6 more
doaj   +1 more source

Rapid, low-cost detection of Zika virus using programmable biomolecular components

, 2018
The recent Zika virus outbreak highlights the need for low-cost diagnostics that can be rapidly developed for distribution and use in pandemic regions.
Dudley, Dawn M., Daringer, Nicole M., Green, Alexander A., Lee, Jeong Wook, Fan, Melina, O'Connor, David H., Ferrante, Tom, Lambert, Guillaume, Pardee, Keith, Braff, Dana, Takahashi, Melissa K., Ma, Duo, Bosch, Irene, Donghia, Nina, Gehrke, Lee, Collins, James J.   +15 more
core   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

G-quadruplex Structures Contribute to Differential Radiosensitivity of the Human Genome

iScience, 2019
Summary: DNA, the fundamental unit of human cell, generally exists in Watson-Crick base-paired B-DNA form. Often, DNA folds into non-B forms, such as four-stranded G-quadruplexes.
Nitu Kumari, Supriya V. Vartak, Sumedha Dahal, Susmita Kumari, Sagar S. Desai, Vidya Gopalakrishnan, Bibha Choudhary, Sathees C. Raghavan   +7 more
doaj   +1 more source