Results 71 to 80 of about 508,313 (310)

Laboratory of molecular biology and clinical biochemistry, Institute of Animal Biology NAAS: the history and present state

, 2020
The Laboratory of molecular biology and clinical biochemistry was established with the foundation of Scientific Production Center (SPC) for the study of prion infections in 2001.
I. M. Petrukh, D. D. Ostapiv, M. R. Kozak, Yu. V. Martyn, N. V. Kuzmina, V. V. Vlizlo   +5 more
core   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

TonEBP Regulates PCNA Polyubiquitination in Response to DNA Damage through Interaction with SHPRH and USP1

iScience, 2019
Summary: Polyubiquitination of proliferating cell nuclear antigen (PCNA) regulates the error-free template-switching mechanism for the bypass of DNA lesions during DNA replication.
Hyun Je Kang, Hyun Park, Eun Jin Yoo, Jun Ho Lee, Soo Youn Choi, Whaseon Lee-Kwon, Kyoo-young Lee, Jin-Hoe Hur, Jeong Kon Seo, Jae Sun Ra, Eun-A. Lee, Kyungjae Myung, Hyug Moo Kwon   +12 more
doaj   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Fluorescence Microscopy for Cilia in Cultured Cells and Zebrafish Embryos

Bio-Protocol, 2014
Cilia are microtubule-based hair-like projections found in organisms, ranging from protozoa to mammals. This protocol provides methods for immunofluorescence staining of cilia in cultured cells and zebrafish embryos.
Jingli Cao, Xueliang Zhu , Xiumin Yan
doaj   +1 more source

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Regulation of glycolysis by Pdk functions as a metabolic checkpoint for cell cycle quiescence in hematopoietic stem cells

, 2013
SummaryDefining the metabolic programs that underlie stem cell maintenance will be essential for developing strategies to manipulate stem cell capacity.
Soga, Tomoyoshi, Ikeda, Eiji, Rahimi, Yasmeen, Nagamatsu, G, Ikeda, E, Kobayashi, Hiroshi, Goda, N, Johnson, RS, Nakamura-Ishizu, Ayako, Suda, Toshio, Takubo, Keiyo, Soga, T, Nakamura-Ishizu, A, Nagamatsu, Go, Goda, Nobuhito, Takubo, K, 池田, 栄二, Kobayashi, CI, Hirao, Atsushi, Kobayashi, H, Rahimi, Y, Suematsu, Makoto, Kobayashi, Chiharu I., Johnson, Randall S.   +23 more
core   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Phospholipase D1 regulates cell migration in a lipase activity-independent manner

, 2006
Cell migration, a complex biological process, requires dynamic cytoskeletal remodeling. Phospholipase D (PLD) generates phosphatidic acid, a lipid second messenger. Although PLD activity has been proposed to play a role in cytoskeletal rearrangement, the
Kim, JH, Pann-Ghill Suh, Kim, HW, Hyeona Jeon, Ryu, SH, Jung Hwan Kim, Suh, Pann-Ghill, Suh, PG, Sung Ho Ryu, Jeon, H, Hyun-wook Kim   +10 more
core   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source