Robust, universal biomarker assay to detect senescent cells in biological specimens
Aging Cell, 2016 Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory.
K. Evangelou +12 more
semanticscholar +1 more source
Probing cellular protein complexes using single-molecule pull-down. [PDF]
, 2011 Proteins perform most cellular functions in macromolecular complexes. The same protein often participates in different complexes to exhibit diverse functionality.
Arauz, Edwin +9 more
core +3 more sources
The Effects of Statistical Multiplicity of Infection on Virus Quantification and Infectivity Assays [PDF]
, 2018 Many biological assays are employed in virology to quantify parameters of interest. Two such classes of assays, virus quantification assays (VQA) and infectivity assays (IA), aim to estimate the number of viruses present in a solution, and the ability of a viral strain to successfully infect a host cell, respectively.
arxiv +1 more source
Capture and light-induced release of antibiotics by an azo dye polymer [PDF]
Sci Rep 10, 3267 (2020), 2019 The isomerisation of azo dyes can induce conformational change which have potential applications in medicine and environmental protection. We developed an agar diffusion assay to test the capture and release of biologically active molecules from an azo electro-optic polymer, Poly (Disperse Red 1 methacrylate) (DR1/PMMA).
arxiv +1 more source
On-chip Acousto Thermal Shift Assay for Rapid and Sensitive Assessment of Protein Thermodynamic Stability [PDF]
, 2021 Thermal shift assays (TSAs) have been extensively used to study thermodynamics of proteins and provide an efficient means to assess protein-ligand binding or protein-protein interaction. However, existing TSAs have limitations such as time consuming, labor intensive, or low sensitivity.
arxiv +1 more source
RegenBase: a knowledge base of spinal cord injury biology for translational research. [PDF]
, 2016 Spinal cord injury (SCI) research is a data-rich field that aims to identify the biological mechanisms resulting in loss of function and mobility after SCI, as well as develop therapies that promote recovery after injury.
Abeyruwan, Saminda W +9 more
core +2 more sources
Biologically-informed neural networks guide mechanistic modeling from sparse experimental data [PDF]
, 2020 Biologically-informed neural networks (BINNs), an extension of physics-informed neural networks [1], are introduced and used to discover the underlying dynamics of biological systems from sparse experimental data. In the present work, BINNs are trained in a supervised learning framework to approximate in vitro cell biology assay experiments while ...
arxiv +1 more source
Water Practice and Technology, 2022 The possible effects of wastewater treatment products on mussels (Mytilus galloprovincialis) were studied. Three common pollution biomarkers were used: neutral red retention assay, malondialdehyde contents and DNA damage through comet assay.
Aikaterini Itziou
doaj +1 more source
A novel NGS library preparation method to characterize native termini of fragmented DNA. [PDF]
, 2020 Biological and chemical DNA fragmentation generates DNA molecules with a variety of termini, including blunt ends and single-stranded overhangs. We have developed a Next Generation Sequencing (NGS) assay, XACTLY, to interrogate the termini of fragmented ...
Green, Richard E +7 more
core +1 more source
Inference of weak-form partial differential equations describing migration and proliferation mechanisms in wound healing experiments on cancer cells [PDF]
arXiv, 2023 Targeting signaling pathways that drive cancer cell migration or proliferation is a common therapeutic approach. A popular experimental technique, the scratch assay, measures the migration and proliferation-driven cell closure of a defect in a confluent cell monolayer. These assays do not measure dynamic effects.
arxiv