Using detergent to enhance detection sensitivity of African trypanosomes in human CSF and blood by Loop-Mediated Isothermal Amplification (LAMP) [PDF]
, 2011 <p><b>Background:</b> The loop-mediated isothermal amplification (LAMP) assay, with its advantages of simplicity, rapidity and cost effectiveness, has evolved as one of the most sensitive and specific methods for the detection of a ...
A Johannessen +54 more
core +3 more sources
PASSPORT-seq: A Novel High-Throughput Bioassay to Functionally Test Polymorphisms in Micro-RNA Target Sites [PDF]
, 2018 Next-generation sequencing (NGS) studies have identified large numbers of genetic variants that are predicted to alter miRNA-mRNA interactions. We developed a novel high-throughput bioassay, PASSPORT-seq, that can functionally test in parallel 100s of ...
Bhatia, Puja +7 more
core +9 more sources
Convection-Enhanced Biopatterning with Recirculation of Hydrodynamically Confined Nanoliter Volumes of Reagents [PDF]
, 2016 We present a new methodology for efficient and high-quality patterning of biological reagents for surface-based biological assays. The method relies on hydrodynamically confined nanoliter volumes of reagents to interact with the substrate at the micrometer-length scale.
arxiv +1 more source
Journal of antibiotics (Tokyo. 1968), 1989 In a genetic study of Streptomyces lincolnensis, a riboflavin auxotroph, NTG-3, was obtained which was also a lincomycin non-producer under standard antibiotic production conditions.
J. Coats, G. Li, M. Kuo, D. Yurek
semanticscholar +1 more source
Small-scale intraspecific life history variation in herbivorous spider mites (Tetranychus pacificus) is associated with host plant cultivar. [PDF]
, 2013 Life history variation is a general feature of arthropod systems, but is rarely included in models of field or laboratory data. Most studies assume that local processes occur identically across individuals, ignoring any genetic or phenotypic variation in
de Valpine, Perry +3 more
core +2 more sources
Cell Detection by Functional Inverse Diffusion and Non-negative Group Sparsity$-$Part I: Modeling and Inverse Problems [PDF]
IEEE Transactions on Signal Processing, vol. 66, no. 20, pp.
5407-5421, 2018, 2017 In this two-part paper, we present a novel framework and methodology to analyze data from certain image-based biochemical assays, e.g., ELISPOT and Fluorospot assays. In this first part, we start by presenting a physical partial differential equations (PDE) model up to image acquisition for these biochemical assays.
arxiv +1 more source
Determination of Protein Concentration Using Bradford Microplate Protein Quantification Assay [PDF]
Disease and Diagnosis, 2015 Background: Bradford protein assay is popular due to its ease of performance and relative sensitivity. Many researchers and laboratories in Iran use standard assay of Bradford by cuvette.
Rouhollah Valipour Nouroozi +2 more
doaj
All-Optical Assay to Study Biological Neural Networks
Frontiers in Neuroscience, 2018 We introduce a novel all-optical assay for functional studies of biological neural networks in vitro. We created a novel optogenetic construct named OptoCaMP which is a combination of a channelrhodopsin variant (CheRiff) and a red genetically encoded ...
Wardiya Afshar Saber +4 more
doaj +1 more source
Molecules, 2020 The development of solid materials that deliver nitric oxide (NO) are of interest for several therapeutic applications. Nevertheless, due to NO’s reactive nature, rapid diffusion and short half-life, reporting their NO delivery characteristics is rather ...
Rosana V. Pinto +4 more
doaj +1 more source
Mussel eggs as indicators of mutagen exposure in coastal and estuarine environments [PDF]
, 1994 The aim of this study was to develop a short-term genotoxicity assay for monitoring the marine environment for mutagens. Based on the developing eggs and embryos of the marine mussel Mytilus edulis, an important pollution indicator species, the test ...
Dixon, D.R., Pascoe, P.L
core