Results 91 to 100 of about 507,580 (307)

mCerulean3-Based Cameleon Sensor to Explore Mitochondrial Ca2+ Dynamics In Vivo

iScience, 2019
Summary: Genetically Encoded Ca2+ Indicators (GECIs) are extensively used to study organelle Ca2+ homeostasis, although some available probes are still plagued by a number of problems, e.g., low fluorescence intensity, partial mistargeting, and pH ...
Elisa Greotti, Ilaria Fortunati, Diana Pendin, Camilla Ferrante, Luisa Galla, Lorena Zentilin, Mauro Giacca, Nina Kaludercic, Moises Di Sante, Letizia Mariotti, Annamaria Lia, Marta Gómez-Gonzalo, Michele Sessolo, Giorgio Carmignoto, Renato Bozio, Tullio Pozzan   +15 more
doaj   +1 more source

Epigenetic blind spots – the role of DNA methylation dynamics in stem cell‐based models of embryogenesis

FEBS Letters, EarlyView.
Embryo‐like structures (stembryos) are an innovative tool, but they are hindered by experimental variability and limited developmental potential. DNA methylation is crucial for mammalian development, but its status in stembryo models is poorly characterized.
Sara Canil, Anna Corrà, Annalisa Scapolatiello, Chengxiang Qiu, Gianluca Amadei   +4 more
wiley   +1 more source

COMPARISON OF BIG DATA ANALYTICS TOOLS: A BIOINFORMATICS CASE STUDY

FUUAST Journal of Biology, 2021
Due to the exponential growth of genomic sequence in the field of biological science, it becomes the immense challenge for biomedical practitioners and researchers to access and analyze it.
MUHAMMAD SHAHZAD, KAMRAN AHSAN
doaj  

Colib’read on galaxy: a tools suite dedicated to biological information extraction from raw NGS reads

, 2016
International audienceBackground: With next-generation sequencing (NGS) technologies, the life sciences face a deluge of raw data.Classical analysis processes for such data often begin with an assembly step, needing large amounts of computingresources ...
Rivals, Eric, Marchet, Camille, Camille Marchet, Vincent Miele, Pierre Peterlongo, Collin, Olivier, Claire Lemaitre, Raluca Uricaru, Salmela, Leena, Alexan Andrieux, Bastien Cazaux, Sacomoto, Gustavo, Le Bras, Yvan, Cyril Monjeaud, Leena Salmela, Miele, Vincent, Monjeaud, Cyril, Cazaux, Bastien, Andrieux, Alexan, Olivier Collin, El Aabidine, Amal Zine, Lacroix, Vincent, Alves Carvalho, Susete, Yvan Le Bras, Susete Alves-Carvalho, Uricaru, Raluca, Gustavo Sacomoto, Lemaitre, Claire, Éric Rivals, Vincent Lacroix, Peterlongo, Pierre, Amal Zine El Aabidine, Alves-Carvalho, Susete   +32 more
core   +1 more source

Biophysical approaches for studying viral entry

FEBS Letters, EarlyView.
Viruses infect all living organisms and have been responsible for major epidemics and pandemics. Their ongoing evolutionary battle with host defenses creates a constant need for improved tools to study viral behavior. Advancing methods to probe viral attachment, fusion, and genome release deepen our understanding of how infections begin and support the
Inbar Yosibash, Raya Sorkin
wiley   +1 more source

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity

FEBS Letters, EarlyView.
Septin 9 polybasic domains couple phosphoinositide‐rich membrane binding to centrosome positioning, Golgi organization, and microtubule acetylation to control epithelial polarity. Their loss disrupts this axis, causing centrosome mispositioning, Golgi fragmentation, reduced microtubule acetylation, and polarity inversion via upregulation of the ...
Ting ting Cai, Mohyeddine Omrane, Nassima Benzoubir, Didier Samuel, Ama Gassama‐Diagne   +4 more
wiley   +1 more source

Emerging chemical tools and techniques for tracking biological manganese

, 2019
International audienceRecent developments in Mn biology have added new physiological and pathophysiological roles of this essential metal ion to the already existing repertoire of indispensable biological roles of Mn ions.
Das, Sayani, Datta, Ankona, Carmona, Asuncion, Bakthavatsalam, Subha, Ortega, Richard, Rakshit, Ananya, Khatua, Kaustav, Sarkar, Anindita   +7 more
core   +1 more source

Tagging of RPS9 as a tool for ribosome purification and identification of ribosome-associated proteins

, 2020
Ribosomes, the catalytic machinery required for protein synthesis, are comprised of 4 ribosomal RNAs and about 80 ribosomal proteins in mammals. Ribosomes further interact with numerous associated factors that regulate their biogenesis and function.
Stoecklin, Georg, Poetz, Fabian, Jovanović, Bogdan, Schubert, Lisa   +3 more
core   +1 more source

Talk, tools, and tensions: observing biological talk over time [PDF]

, 2010
The goal of this study is to explore new tools for analyzing scientific sense making in out-of-school settings. Although such measures are now common in science classroom research, dialogically-based methodological approaches are relatively new to ...
Wheaton, Mele, Brandt, Carol, Ash, Doris, Loomis, Molly, Crain, Rhiannon, Bennett, Christine   +5 more
core   +1 more source

Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations

FEBS Letters, EarlyView.
The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas, Brigitta Maruzs, Zsófia E. Kálmán, Tomas Klumpler, Gyula Batta, Bálint Péterfia, Zoltán Gáspári   +6 more
wiley   +1 more source