Results 181 to 190 of about 507,580 (307)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Biological variation of baseline serum cortisol concentration in healthy dogs, part 2: clinical applications. [PDF]

Front Vet Sci
Wicker S, Craig S, Albright JD, Springer C, Freeman K, Giori L.   +5 more
europepmc   +1 more source

BACTERIAL CONTACT INDUCES MORPHOLOGICAL AND FUNCTIONAL CHANGES IN PRIMARY NEURAL CORTICAL CELL CULTURES

IBRO Neuroscience Reports, 2023
Juan Lombardo-Hernández, David Muñoz-Rodríguez, Marwane Bourqqia-Ramzi, Pablo Garcia-Pérez, Antonio Murciano, Stefano Geuna, Francisco Conejero, Alvaro Mateos González, M.T Garcia-Esteban, Celia Herrera-Rincón   +9 more
doaj   +1 more source

Bioinformatics pre-selection of thioredoxin/glutaredoxin target proteins for the construction of cellular redox regulatory network

, 2012
Lee H-M. Bioinformatics pre-selection of thioredoxin/glutaredoxin target proteins for the construction of cellular redox regulatory network. Bielefeld: Bielefeld University; 2012.The gradually accumulated knowledge of molecular interaction is assembled ...
Lee, Hang-Mao
core  

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

FEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman, Katarzyna Stapor, Leszek Konieczny, Krzysztof Gądek, Piotr Nowakowski   +4 more
wiley   +1 more source

Toward relational biosecurity: understanding AI-enabled biology as a connected system. [PDF]

Front Microbiol
Holko M.
europepmc   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Ecology is not yet ready for AI-and why that matters. [PDF]

Proc Natl Acad Sci U S A
Mishra G.
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Ten common mistakes that could ruin your enrichment analysis. [PDF]

PLoS Comput Biol
Bora A, McKenzie M, Ziemann M.
europepmc   +1 more source