Results 11 to 20 of about 16,494,787 (294)

Rapid detection of Staphylococcus aureus and Streptococcus pneumoniae by real-time analysis of volatile metabolites

iScience, 2022
Summary: Early detection of pathogenic bacteria is needed for rapid diagnostics allowing adequate and timely treatment of infections. In this study, we show that secondary electrospray ionization–high resolution mass spectrometry (SESI-HRMS) can be used ...
Alejandro Gómez-Mejia, Kim Arnold, Julian Bär, Kapil Dev Singh, Thomas C. Scheier, Silvio D. Brugger, Annelies S. Zinkernagel, Pablo Sinues   +7 more
doaj   +1 more source

Spatio-selective activation of nuclear translocation of YAP with light directs invasion of cancer cell spheroids

iScience, 2021
Summary: The mechanical properties of the extracellular matrix strongly influence tumor progression and invasion. Yes-associated protein (YAP) has been shown to be a key regulator of this process translating mechanical cues from the extracellular matrix ...
Bernhard Illes, Adrian Fuchs, Florian Gegenfurtner, Evelyn Ploetz, Stefan Zahler, Angelika M. Vollmar, Hanna Engelke   +6 more
doaj   +1 more source

Polyvalent guide RNAs for CRISPR antivirals

iScience, 2022
Summary: CRISPR effector Cas13 recognizes and degrades RNA molecules that are complementary to its guide RNA (gRNA) and possesses potential as an antiviral biotechnology because it can degrade viral mRNA and RNA genomes.
Rammyani Bagchi, Rachel Tinker-Kulberg, Mohammad Salehin, Tinku Supakar, Sydney Chamberlain, Ayalew Ligaba-Osena, Eric A. Josephs   +6 more
doaj   +1 more source

A knowledge empowered explainable gene ontology fingerprint approach to improve gene functional explication and prediction

iScience, 2023
Summary: Functional explication of genes is of great scientific value. However, conventional methods have challenges for those genes that may affect biological processes but are not annotated in public databases.
Ying Wang, Hui Zong, Fan Yang, Yuantao Tong, Yujia Xie, Zeyu Zhang, Honglian Huang, Rongbin Zheng, Shuangkuai Wang, Danqi Huang, Fanglin Tan, Shiyang Cheng, M. James C. Crabbe, Xiaoyan Zhang   +13 more
doaj   +1 more source

Development of small fluorescent probes for the analysis of autophagy kinetics

iScience, 2023
Summary: Autophagy is a dynamic process that degrades subcellular constituents, and its activity is measured by autophagic flux. The tandem proteins RFP-GFP-LC3 and GFP-LC3-RFP-LC3ΔG, which enable the visualization of autophagic vacuoles of different ...
Hajime Tajima Sakurai, Hidefumi Iwashita, Satoko Arakawa, Alifu Yikelamu, Mizuki Kusaba, Satoshi Kofuji, Hiroshi Nishina, Munetaka Ishiyama, Yuichiro Ueno, Shigeomi Shimizu   +9 more
doaj   +1 more source

Automated design of gene circuits with optimal mushroom-bifurcation behavior

iScience, 2023
Summary: Recent advances in synthetic biology are enabling exciting technologies, including the next generation of biosensors, the rational design of cell memory, modulated synthetic cell differentiation, and generic multifunctional biocircuits.
Irene Otero-Muras, Ruben Perez-Carrasco, Julio R. Banga, Chris P. Barnes   +3 more
doaj   +1 more source

Mass spectrometry-based proteomics as a tool to identify biological matrices in forensic science [PDF]

International Journal of Legal Medicine, 2012
In forensic casework analysis, identification of the biological matrix and the species of a forensic trace, preferably without loss of DNA, is of major importance. The biological matrices that can be encountered in a forensic context are blood (human or non-human), saliva, semen, vaginal fluid, and to a lesser extent nasal secretions, feces, and urine.
Van Steendam, Katleen, De Ceuleneer, Marlies, Dhaenens, Maarten, Van Hoofstat, David, Deforce, Dieter   +4 more
openaire   +3 more sources

Nanocourses: A Short Course Format as an Educational Tool in a Biological Sciences Graduate Curriculum [PDF]

CBE—Life Sciences Education, 2008
Traditional courses for graduate students in the biological sciences typically span a semester, are organized around the fundamental concepts of a single discipline, and are aimed at the needs of incoming students. Such courses demand significant time commitment from both faculty and course participants; thus, they are avoided by a subset of the ...
Anna M, Bentley, Spyros, Artavanis-Tsakonas, Jennifer S, Stanford   +2 more
openaire   +2 more sources

Data Management Plan for a Biotechnology and Biological Sciences Research Council (BBSRC) Tools and Resources Development Fund (TRDF) Grant [PDF]

Research Ideas and Outcomes, 2017
This Data Management Plan (DMP) was created for Laurent Gatto's BBSRC Tools and Resources Development Fund award (BB/N023129/1).  The DMP describes the management and sharing of all data and code associated with the grant, including software dissemination and release schedule, source code development and open source licensing, software ...
Laurent Gatto
doaj   +4 more sources

evoText: A new tool for analyzing the biological sciences

Studies in History and Philosophy of Science Part C: Studies in History and Philosophy of Biological and Biomedical Sciences, 2016
We introduce here evoText, a new tool for automated analysis of the literature in the biological sciences. evoText contains a database of hundreds of thousands of journal articles and an array of analysis tools for generating quantitative data on the nature and history of life science, especially ecology and evolutionary biology. This article describes
Ramsey, Grant, Pence, Charles H.
openaire   +3 more sources