Results 31 to 40 of about 16,494,787 (294)

Applications of CRISPR-Cas9 as an Advanced Genome Editing System in Life Sciences

BioTech, 2021
Targeted nucleases are powerful genomic tools to precisely change the target genome of living cells, controlling functional genes with high exactness. The clustered regularly interspaced short palindromic repeats associated protein 9 (CRISPR-Cas9) genome
Kamand Tavakoli, Alireza Pour-Aboughadareh, Farzad Kianersi, Peter Poczai, Alireza Etminan, Lia Shooshtari   +5 more
doaj   +1 more source

Appraisal of ozone as biologically active molecule and experimental tool in biomedical sciences

Medicinal Chemistry Research, 2010
Ozone (O3) (CAS 10028-15-6) is a major air industrial pollutant and is well known for its very strong oxidative actions which affords the molecule its useful antimicrobial and deodorizing properties, but also its potential toxic effects. Knowledge of the activity and safety of ozone is important if its potential for use as a biologically active agent ...
Mokoena, Mmalebuso L., Brink, Christiaan B., Harvey, Brian Herbert, Oliver, Douglas W.   +3 more
openaire   +3 more sources

Biological X-ray absorption spectroscopy (BioXAS): a valuable tool for the study of trace elements in the life sciences [PDF]

Current Opinion in Structural Biology, 2008
Using X-ray absorption spectroscopy (XAS) the binding modes (type and number of ligands, distances and geometry) and oxidation states of metals and other trace elements in crystalline as well as non-crystalline samples can be revealed. The method may be applied to biological systems as a 'stand-alone' technique, but it is particularly powerful when ...
Richard W, Strange, Martin C, Feiters
openaire   +2 more sources

Context-dependent 'safekeeping' of foraging tools in New Caledonian crows [PDF]

, 2015
This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC; David Phillips Fellowship BB/G023913/2; C.R.), and studentships from the BBSRC (B.K.) and the University of St Andrews (J.v.d.W.).Several animal species use ...
James J. H. St Clair, Jessica E. M. van der Wal, van der Wal, Jessica Eva Megan, Klump, B., Christian Rutz, Rutz, Christian, St Clair, James, van der Wal, J., Klump, Barbara Christina, Barbara C. Klump, St Clair, J., Rutz, C.   +11 more
core   +1 more source

A robust vessel-labeling pipeline with high tissue clearing compatibility for 3D mapping of vascular networks

iScience
Summary: The combination of vessel-labeling, tissue-clearing, and light-sheet imaging techniques provides a potent tool for accurately mapping vascular networks, enabling the assessment of vascular remodeling in vascular-related disorders.
Yating Deng, Jingtan Zhu, Xiaomei Liu, Junyao Dai, Tingting Yu, Dan Zhu   +5 more
doaj   +1 more source

Developing ABEmax-NG with Precise Targeting and Expanded Editing Scope to Model Pathogenic Splice Site Mutations In Vivo

iScience, 2019
Summary: RNA splicing is related to many human diseases; however, lack of efficient genetic approaches to modulate splicing has prevented us from dissecting their functions in human diseases.
Shisheng Huang, Zhaodi Liao, Xiangyang Li, Zhen Liu, Guanglei Li, Jianan Li, Zongyang Lu, Yu Zhang, Xiajun Li, Xu Ma, Qiang Sun, Xingxu Huang   +11 more
doaj   +1 more source

Computational Tools for Discovering and Engineering Natural Product Biosynthetic Pathways

iScience, 2020
Natural products (NPs), also known as secondary metabolites, are produced in bacteria, fungi, and plants. NPs represent a rich source of antibacterial, antifungal, and anticancer agents.
Hengqian Ren, Chengyou Shi, Huimin Zhao
doaj   +1 more source

Interlaboratory consistency of SDC2 promoter methylation detection in colorectal cancer using the post-optimized materials

iScience
Summary: Fecal DNA-based Syndecan 2 (SDC2) methylation detection is a promising non-invasive strategy for early colorectal cancer (CRC) screening. In China, commercial assays for SDC2 methylation detection vary in sensitivity and specificity, yet there ...
Lijing Zhang, Duo Wang, Ziqiang Li, Guigao Lin, Jinming Li, Rui Zhang   +5 more
doaj   +1 more source

Label-free 3D-CLEM Using Endogenous Tissue Landmarks

iScience, 2018
Summary: Emerging 3D correlative light and electron microscopy approaches enable studying neuronal structure-function relations at unprecedented depth and precision.
Manja Luckner, Steffen Burgold, Severin Filser, Maximilian Scheungrab, Yilmaz Niyaz, Eric Hummel, Gerhard Wanner, Jochen Herms   +7 more
doaj   +1 more source

All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures

iScience, 2019
Summary: All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to
Chiao Huang, Chu-Yi Tai, Kai-Ping Yang, Wei-Kun Chang, Kuo-Jen Hsu, Ching-Chun Hsiao, Shun-Chi Wu, Yen-Yin Lin, Ann-Shyn Chiang, Shi-Wei Chu   +9 more
doaj   +1 more source