Results 11 to 20 of about 1,087,486 (267)
Evaluating methods and protocols of ferritin-based magnetogenetics
iScience, 2021 Summary: FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins ...
Miriam Hernández-Morales +3 more
doaj +1 more source
Deciphering cellular signals in adult mouse sinoatrial node cells
iScience, 2022 Summary: Sinoatrial node (SAN) cells are the pacemakers of the heart. This study describes a method for culturing and infection of adult mouse SAN cells with FRET-based biosensors that can be exploited to examine signaling events.
Gopireddy R. Reddy +10 more
doaj +1 more source
A contamination focused approach for optimizing the single-cell RNA-seq experiment
iScience, 2023 Summary: Droplet-based single-cell RNA-seq (scRNA-seq) data are plagued by ambient contaminations caused by nucleic acid material released by dead and dying cells.
Deronisha Arceneaux +12 more
doaj +1 more source
Plasma contains ultrashort single-stranded DNA in addition to nucleosomal cell-free DNA
iScience, 2022 Summary: Plasma cell-free DNA is being widely explored as a biomarker for clinical screening. Currently, methods are optimized for the extraction and detection of double-stranded mononucleosomal cell-free DNA of ∼160bp in length. We introduce uscfDNA-seq,
Jordan Cheng +13 more
doaj +1 more source
Tissue clearing and imaging methods for cardiovascular development
iScience, 2021 Summary: Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different
Hana Kolesová +4 more
doaj +1 more source
Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
iScience, 2023 Summary: To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield ...
Christoph Drobek +5 more
doaj +1 more source
Sensitive label-free imaging of brain samples using FxClear-based tissue clearing technique
iScience, 2021 Summary: Optical clearing has emerged as a powerful tool for volume imaging. Although volume imaging with immunostaining have been successful in many protocols, yet obtaining homogeneously stained thick samples remains challenging.
Boram Lee +12 more
doaj +1 more source
iScience, 2022 Summary: The choroid plexus (CP) is a highly vascularized structure containing endothelial and epithelial cells located in the ventricular system of the central nervous system (CNS).
Walter Muranyi +9 more
doaj +1 more source
The proteomic profile of the human myotendinous junction
iScience, 2022 Summary: Proteomics analysis of skeletal muscle has recently progressed from whole muscle tissue to single myofibers. Here, we further focus on a specific myofiber domain crucial for force transmission from muscle to tendon, the myotendinous junction ...
Anders Karlsen +8 more
doaj +1 more source
iScience, 2022 Summary: Quantitative features of GLUT4 glucose transporter’s behavior deep inside cells remain largely unknown. Our previous analyses with live-cell imaging of intracellular GLUT4 trafficking demonstrated two crucial early events responsible for ...
Hiroyasu Hatakeyama +2 more
doaj +1 more source