Combining Experimental Data and Computational Methods for the Non-Computer Specialist
Molecules, 2020 Experimental methods are indispensable for the study of the function of biological macromolecules, not just as static structures, but as dynamic systems that change conformation, bind partners, perform reactions, and respond to different stimulus ...
Reinier Cárdenas +2 more
doaj +3 more sources
Tissue clearing and imaging methods for cardiovascular development
iScience, 2021 Summary: Tissue imaging in 3D using visible light is limited and various clearing techniques were developed to increase imaging depth, but none provides universal solution for all tissues at all developmental stages. In this review, we focus on different
Hana Kolesová +4 more
doaj +3 more sources
New Author Guidelines for Displaying Data and Reporting Data Analysis and Statistical Methods in Experimental Biology [PDF]
Drug Metabolism and Disposition, 2020 The American Society for Pharmacology and Experimental Therapeutics has revised the Instructions to Authors for Drug Metabolism and Disposition, Journal of Pharmacology and Experimental Therapeutics, and Molecular Pharmacology These revisions relate to data analysis (including statistical analysis) and reporting but do not tell investigators how to ...
Martin C. Michel +2 more
openaire +8 more sources
Genre innovation and multimodal expression in scholarly communication: Video methods articles in experimental biology [PDF]
Ibérica, 2018 Digital media have the potential to foster genre innovation and change in scholarly communication, by 1) opening up new, diversified audiences to academics, and 2) facilitating the use of a range of multimodal semiotic resources, that combine word and ...
Christoph A. Hafner
doaj +1 more source
Evaluating methods and protocols of ferritin-based magnetogenetics
iScience, 2021 Summary: FeRIC (Ferritin iron Redistribution to Ion Channels) is a magnetogenetic technique that uses radiofrequency (RF) alternating magnetic fields to activate the transient receptor potential channels, TRPV1 and TRPV4, coupled to cellular ferritins ...
Miriam Hernández-Morales +3 more
doaj +1 more source
Deciphering cellular signals in adult mouse sinoatrial node cells
iScience, 2022 Summary: Sinoatrial node (SAN) cells are the pacemakers of the heart. This study describes a method for culturing and infection of adult mouse SAN cells with FRET-based biosensors that can be exploited to examine signaling events.
Gopireddy R. Reddy +10 more
doaj +1 more source
A contamination focused approach for optimizing the single-cell RNA-seq experiment
iScience, 2023 Summary: Droplet-based single-cell RNA-seq (scRNA-seq) data are plagued by ambient contaminations caused by nucleic acid material released by dead and dying cells.
Deronisha Arceneaux +12 more
doaj +1 more source
Plasma contains ultrashort single-stranded DNA in addition to nucleosomal cell-free DNA
iScience, 2022 Summary: Plasma cell-free DNA is being widely explored as a biomarker for clinical screening. Currently, methods are optimized for the extraction and detection of double-stranded mononucleosomal cell-free DNA of ∼160bp in length. We introduce uscfDNA-seq,
Jordan Cheng +13 more
doaj +1 more source
Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
iScience, 2023 Summary: To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield ...
Christoph Drobek +5 more
doaj +1 more source
Sensitive label-free imaging of brain samples using FxClear-based tissue clearing technique
iScience, 2021 Summary: Optical clearing has emerged as a powerful tool for volume imaging. Although volume imaging with immunostaining have been successful in many protocols, yet obtaining homogeneously stained thick samples remains challenging.
Boram Lee +12 more
doaj +1 more source