Results 21 to 30 of about 1,087,486 (267)

Precision vibratome for high-speed ultrathin biotissue cutting and organ-wide imaging

iScience, 2021
Summary: Cutting tissues into ultrathin slices is highly desired in sectioning-based organ-wide imaging. However, it is difficult to perform tissue cutting at a high speed with excellent quality.
Yafeng Li, Zhangheng Ding, Lei Deng, Guoqing Fan, Qi Zhang, Hui Gong, Anan Li, Jing Yuan, Jianwei Chen   +8 more
doaj   +1 more source

Multiplexed protein profiling reveals spatial subcellular signaling networks

iScience, 2022
Summary: Protein-protein interaction networks are altered in multi-gene dysregulations in many disorders. Image-based protein multiplexing sheds light on signaling pathways to dissect cell-to-cell heterogeneity, previously masked by the bulk assays ...
Shuangyi Cai, Thomas Hu, Mythreye Venkatesan, Mayar Allam, Frank Schneider, Suresh S. Ramalingam, Shi-Yong Sun, Ahmet F. Coskun   +7 more
doaj   +1 more source

Scale space detector for analyzing spatiotemporal ventricular contractility and nuclear morphogenesis in zebrafish

iScience, 2022
Summary: In vivo quantitative assessment of structural and functional biomarkers is essential for characterizing the pathophysiology of congenital disorders.
Tanveer Teranikar, Cameron Villarreal, Nabid Salehin, Toluwani Ijaseun, Jessica Lim, Cynthia Dominguez, Vivian Nguyen, Hung Cao, Cheng–Jen Chuong, Juhyun Lee   +9 more
doaj   +1 more source

Combining Experimental Data and Computational Methods for the Non-Computer Specialist

Molecules, 2020
Experimental methods are indispensable for the study of the function of biological macromolecules, not just as static structures, but as dynamic systems that change conformation, bind partners, perform reactions, and respond to different stimulus ...
Reinier Cárdenas, Javier Martínez-Seoane, Carlos Amero   +2 more
doaj   +1 more source

Direct capture and sequencing reveal ultra-short single-stranded DNA in biofluids

iScience, 2022
Summary: Cell-free DNA (cfDNA) has become the predominant analyte of liquid biopsy; however, recent studies suggest the presence of subnucleosomal-sized DNA fragments in circulation that are likely single-stranded.
Lauren Y. Cheng, Peng Dai, Lucia R. Wu, Abhijit A. Patel, David Yu Zhang   +4 more
doaj   +1 more source

Spatial accuracy evaluation of magnetic source imaging methods on OPM-based MEG

iScience, 2022
Summary: The emergence of the optically pumped magnetometer (OPM)-based magnetoencephalography (MEG) has led to new developments in MEG technology.
Nan An, Fuzhi Cao, Wen Li, Wenli Wang, Weinan Xu, Chunhui Wang, Min Xiang, Yang Gao, Binbin Sui, Dawei Wang, Dexin Yu, Xiaolin Ning   +11 more
doaj   +1 more source

PCR-based profiling of transcription factor activity in vivo by a virus-based reporter battery

iScience, 2022
Summary: Understanding the molecular mechanisms of gene regulation is pivotal for understanding how cells establish and modify their identities and functions.
Hitomi Abe, Kentaro Abe
doaj   +1 more source

Statistical methods to evaluate discrete Boolean mathematical models from systems biology experimental data sets

IFAC-PapersOnLine
Antonio Bensussen, J. Arturo Arciniega-González, Rosario Pacheco-Marín, Elena R. Álvarez-Buylla, Juan Carlos Martínez-García   +4 more
openaire   +2 more sources

Specificity and efficiency of tamoxifen-mediated Cre induction is equivalent regardless of age

iScience, 2023
Summary: Temporally controlling Cre recombination through tamoxifen (Tam) induction has many advantages for biomedical research. Most studies report early post-natal/juvenile (12 m.o.).
Collyn M. Kellogg, Kevin Pham, Sunghwan Ko, Jillian E.J. Cox, Adeline H. Machalinski, Michael B. Stout, Amanda L. Sharpe, Michael J. Beckstead, Ana J. Chucair-Elliott, Sarah R. Ocañas, Willard M. Freeman   +10 more
doaj   +1 more source

Fabrication of a new all-in-one microfluidic dielectrophoresis integrated chip and living cell separation

iScience, 2022
Summary: Microfluidic dielectrophoresis (DEP) technology has been applied to many devices to perform label-free target cell separation. Cells separated by these devices are used in laboratories, mainly for medical research.
Kyoichi Oshiro, Yoshikazu Wakizaka, Masayo Takano, Takayuki Itoi, Hiroki Ohge, Kazumi Koba, Kyoko Yarimizu, So Fujiyoshi, Fumito Maruyama   +8 more
doaj   +1 more source