Results 251 to 260 of about 6,926,142 (285)

Bioluminescence imaging: looking beyond the light

Trends in Molecular Medicine, 2012
Bioluminescence imaging (BLI) enables in vivo imaging of molecular and cellular processes. It has gained in popularity over the past decade because of its easy translation from in vitro to in vivo experiments, its sensitivity, and its ease of use.
Keyaerts, Marleen, Caveliers, Vicky, Lahoutte, Tony   +2 more
openaire   +3 more sources

Cellular Bioluminescence Imaging

Cold Spring Harbor Protocols, 2012
Bioluminescence imaging of live cells has recently been recognized as an important alternative to fluorescence imaging. Fluorescent probes are much brighter than bioluminescent probes (luciferase enzymes) and, therefore, provide much better spatial and temporal resolution and much better contrast for delineating cell structure.
David K. Welsh, Takako Noguchi
openaire   +1 more source

Pyridone Luciferins and Mutant Luciferases for Bioluminescence Imaging

ChemBioChem, 2018
New applications for bioluminescence imaging require an expanded set of luciferase enzymes and luciferin substrates. Here, we report two novel luciferins for use in vitro and in cells.
Jennifer Prescher, Krysten Jones
exaly   +2 more sources

Validation of Bioluminescent Imaging Techniques

2009
Bioluminescence imaging (BLI) is frequently cited for its ease of quantification. This fundamental strength of BLI has led to applications in cancer research, cell transplantation, and monitoring of infectious disease in which bioluminescence intensity is correlated with other metrics. However, bioluminescence measurements can be influenced by a number
John, Virostko, E Duco, Jansen
openaire   +2 more sources

Image-guided simulation for bioluminescence tomographic imaging

SPIE Proceedings, 2005
Noninvasive imaging of the reporter gene expression based on bioluminescence is playing an important role in the areas of cancer biology, cell biology, and gene therapy. The central problem for the bioluminescence tomography (BLT) we are developing is to reconstruct the underlying bioluminescent source distribution in a small animal using a modality ...
Kumar Durairaj, Wenxiang Cong, Jacqueline Thiesse, Earl Nixon, John Meinel, Alexander X. Cong, Geoffrey McLennan, Eric A. Hoffman, Jiang Ming 0001, Ge Wang 0001   +9 more
openaire   +1 more source

Bioluminescence imaging of selenocysteine in vivo with a highly sensitive probe.

ACS Sensors, 2019
Selenocysteine (Sec), a vital member of reactive selenium species, is closely implicated in diverse pathophysiological states, including cancer, cardiovascular disease, diabetes, neurodegenerative diseases, and male infertility. Monitoring Sec in vivo is
Ling Zhang, Yanfen Shi, Zhijia Sheng, Yiran Zhang, Xiaoning Kai, Minyong Li, Xiaoxin Yin   +6 more
semanticscholar   +1 more source

Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells

Nature Methods, 2023
Li Jiang, Xin Xie, Ni Su, Dasheng Zhang, Xianjun Chen, Xiaochen Xu, Bibi Zhang, Kaiyi Huang, Jingwei Yu, Mengyue Fang, Bingkun Bao, Fangting Zuo, Lipeng Yang, Rui Zhang, Huiwen Li, Xinyi Huang, Zhengda Chen, Qi Zeng, Renmei Liu, Qiuning Lin, Yuzheng Zhao, Aiming Ren, Linyong Zhu, Yi Yang   +23 more
semanticscholar   +1 more source

Noninvasive Bioluminescent Imaging of Infections

2009
Traditional studies of viral and bacterial infection and pathogenesis have generally relied on animal models that require the sacrifice of infected animals to determine viral or bacterial distributions and titers. The recent application of the in vivo bioluminescence imaging (BLI) to monitor the replication and tropism of pathogens expressing the ...
openaire   +2 more sources

Using bioluminescence imaging in glioma research

Journal of Clinical Neuroscience, 2015
Glioblastoma multiforme (GBM) is the most common malignant brain tumour and has the worst prognosis. Over the last decade, the use of bioluminescence imaging technology has rapidly become widespread to further understand the mechanisms that drive GBM development and progression.
Rodney B, Luwor, Stanley S, Stylli, Andrew H, Kaye   +2 more
openaire   +2 more sources

Optical Bioluminescence Protocol for Imaging Mice

2018
The presence, growth, or decline of transfected cell populations expressing the enzyme Luciferase can be followed in live mice using bioluminescence optical imaging techniques. This protocol describes how to verify the imaging equipment, options for injecting the substrate Luciferin into mice, image acquisition considerations, and commonly used data ...
David, Stout, John, David
openaire   +2 more sources