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Using bioluminescence imaging in glioma research

Journal of Clinical Neuroscience, 2015
Glioblastoma multiforme (GBM) is the most common malignant brain tumour and has the worst prognosis. Over the last decade, the use of bioluminescence imaging technology has rapidly become widespread to further understand the mechanisms that drive GBM development and progression.
Rodney B, Luwor   +2 more
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In Vivo Bacterial Imaging Using Bioluminescence

2018
Bacterial luminescence allows for noninvasive continuous monitoring of promoter activity in a wide range of model systems. This chapter details various examples of use of the lux reporter system to measure promoter activity in bacteria using the vector pUC18T-mini-Tn7T-lux-Tp.
Mariette, Barbier   +2 more
openaire   +2 more sources

Optical Bioluminescence Protocol for Imaging Mice

2018
The presence, growth, or decline of transfected cell populations expressing the enzyme Luciferase can be followed in live mice using bioluminescence optical imaging techniques. This protocol describes how to verify the imaging equipment, options for injecting the substrate Luciferin into mice, image acquisition considerations, and commonly used data ...
David, Stout, John, David
openaire   +2 more sources

Noninvasive Bioluminescent Imaging of Infections

2009
Traditional studies of viral and bacterial infection and pathogenesis have generally relied on animal models that require the sacrifice of infected animals to determine viral or bacterial distributions and titers. The recent application of the in vivo bioluminescence imaging (BLI) to monitor the replication and tropism of pathogens expressing the ...
openaire   +2 more sources

Bioluminescent Capsules for Live-Cell Imaging

Bioconjugate Chemistry, 2012
A bioluminescent capsule was designed for illuminating cell signaling and protein localization. The capsule consists of four components, namely, a secretion peptide (SP), a luciferase body, a cargo protein (or peptide), and a membrane-localization signal (MLS).
Sung Bae, Kim   +2 more
openaire   +2 more sources

Image-guided simulation for bioluminescence tomographic imaging

SPIE Proceedings, 2005
Noninvasive imaging of the reporter gene expression based on bioluminescence is playing an important role in the areas of cancer biology, cell biology, and gene therapy. The central problem for the bioluminescence tomography (BLT) we are developing is to reconstruct the underlying bioluminescent source distribution in a small animal using a modality ...
Durairaj Kumar   +9 more
openaire   +1 more source

In vivo bioluminescence imaging.

Comparative medicine, 2005
In vivo bioluminescent imaging (BLI) is a versatile and sensitive tool that is based on detection of light emission from cells or tissues. Bioluminescence, the biochemical generation of light by a living organism, is a naturally occurring phenomenon.
Akiko, Sato   +2 more
openaire   +1 more source

Bioluminescence Imaging: Basics and Practical Limitations

2013
Over the last three decades, imaging has been a thriving field with continuous egression of more reliable and highly sophisticated tools and techniques allowing better understanding of biological processes in living organisms. This field continues to expand and its applications broaden to encompass limitless applications in various biomedical research ...
openaire   +2 more sources

Bioluminescence Imaging of Prenylation Inhibition–Letter

Clinical Cancer Research, 2012
We read with great interest the recent article by Chinault and colleagues ([1][1]), providing evidence that aminobisphosphonate zoledronic acid (ZOL) does not exert a direct antitumor activity against breast cancer cells in vivo .
openaire   +2 more sources

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