Results 61 to 70 of about 6,926,142 (285)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

Theranostic protein targeting ErbB2 for bioluminescence imaging and therapy for cancer.

PLoS ONE, 2013
A combination of molecular-targeted cancer imaging and therapy is an emerging strategy to improve cancer diagnosis and minimize the side effects of conventional treatments.
Xiao-Jian Han, Ling-Fei Sun, Yuki Nishiyama, Bin Feng, Hiroyuki Michiue, Masaharu Seno, Hideki Matsui, Kazuhito Tomizawa   +7 more
doaj   +1 more source

Bioluminescence imaging in live cells and animals [PDF]

Neurophotonics, 2016
The use of bioluminescent reporters in neuroscience research continues to grow at a rapid pace as their applications and unique advantages over conventional fluorescent reporters become more appreciated. Here, we describe practical methods and principles for detecting and imaging bioluminescence from live cells and animals.
Jack K, Tung, Ken, Berglund, Claire-Anne, Gutekunst, Ute, Hochgeschwender, Robert E, Gross   +4 more
openaire   +2 more sources

Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system

Proceedings of the National Academy of Sciences of the United States of America, 2019
Significance Bioluminescence is generated by luciferases that oxidize a specific luciferin. The enzymes involved in the synthesis of the luciferin from widespread cellular metabolites have so far been identified for only 2 bioluminescence systems, those ...
Carola Gregor, Jasmin K. Pape, K. Gwosch, Tanja Gilat, S. Sahl, S. Hell   +5 more
semanticscholar   +1 more source

Electrostatically‐Stabilized PEG‐Free Lipid Nanoparticles for Systemic Nucleic Acid Delivery

Advanced Functional Materials, EarlyView.
This work describes non‐PEGylated layered lipid nanoparticles (nonPEG LLNPs), a gene delivery platform that improves upon standard PEGylated LNPs via electrostatic adsorption of charged polymers. nonPEG LLNPs maintain colloidal stability under biological stresses, enhance circulation time, and mitigate accelerated blood clearance and hepatic ...
Namita Nabar, Deeksha Kumaresh, Eduardo Nombera Bueno, Mae Pryor, Paula T. Hammond   +4 more
wiley   +1 more source

Introducing a new reporter gene, membrane-anchored Cypridina luciferase, for multiplex bioluminescence imaging

Molecular Therapy: Oncolytics, 2021
Bioluminescence reporter gene imaging is a robust, high-throughput imaging modality that is useful for tracking cells and monitoring biological processes, both in cell culture and in small animals.
Maxim A. Moroz, Juan Zurita, Anna Moroz, Ekaterina Nikolov, Yury Likar, Konstantin Dobrenkov, Jason Lee, Larissa Shenker, Ronald Blasberg, Inna Serganova, Vladimir Ponomarev   +10 more
doaj   +1 more source

Bio‐Inspired Biopolymeric Implantable Platform for Localized and Sustained Drug Delivery in Solid Tumor Treatment

Advanced Functional Materials, EarlyView.
A bio‐inspired biopolymeric implantable drug delivery platform enables localized and sustained release of 7‐ethyl‐10‐hydroxycamptothecin (SN‐38) in solid tumor treatment. The three‐dimensional (3D) structured crosslinked‐chitosan implants are fabricated via 3D‐printed molds and provide drug release over 3 months, significantly inhibiting tumor growth ...
Mercedes Lozano‐Garcia, Constantine Vogiatzis, Alejandra Planells‐Devesa, Emre Dikici, Caroline Vieira Maluf, Vasudev Vivekanand Nayak, Paulo G. Coelho, Lukasz Witek, Alicia Bilbao, Daniel Bilbao, Prasoon Mohan, Sapna Deo, Sylvia Daunert   +12 more
wiley   +1 more source

Near-infrared dual bioluminescence imaging in mouse models of cancer using infraluciferin

eLife, 2019
Bioluminescence imaging (BLI) is ubiquitous in scientific research for the sensitive tracking of biological processes in small animal models. However, due to the attenuation of visible light by tissue, and the limited set of near-infrared bioluminescent ...
Cassandra L Stowe, T. Burley, H. Allan, M. Vinci, Gabriela Kramer-Marek, Daniela M. Ciobota, G. Parkinson, T. Southworth, G. Agliardi, A. Hotblack, M. Lythgoe, B. Branchini, T. Kalber, James C. Anderson, M. Pule   +14 more
semanticscholar   +1 more source

Volatile Relay Biosensing and Mobile Phone Imaging for Detection of Influenza Virus

Advanced Functional Materials, EarlyView.
Detection of volatile organic compounds is performed via a coupled enzymatic assay with mobile phone detection of the signal. Specific application demonstrated herein is the detection of influenza virus, across multiple levels of biological complexity, up to patient‐derived samples.
Dante G. Andersen, Josefine H. Jakobsen, Mikkel Høj Nielsen, Shreyans Chatterjee, Detlef Michel, Jan Münch, Rüdiger Gross, Alexander N. Zelikin   +7 more
wiley   +1 more source

Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging

International Journal of Biomedical Imaging, 2010
The peak signal or the signal at a predetermined, fixed time point after D-luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging.
Yusuke Inoue, Shigeru Kiryu, Makoto Watanabe, Arinobu Tojo, Kuni Ohtomo   +4 more
doaj   +1 more source