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Bioluminescence Resonance Energy Transfer to Monitor Protein-Protein Interactions
2006The bioluminescence resonance energy transfer (BRET) methodology allows for the study of protein-protein interactions as well as conformational changes within proteins or molecular complexes. BRET is a highly versatile technique that can be applied to in vitro studies using purified proteins, crude cell membranes, cell fractions obtained by ...
Tarik, Issad, Ralf, Jockers
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Biomacromolecules, 2017
Conventional photodynamic therapy is severely constrained by the limited light-penetration depth in tissue. Here, we show efficient photodynamic therapy (PDT) mediated by bioluminescence resonance energy transfer (BRET) that overcomes the light-penetration limitation.
Yingkun Yang +5 more
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Conventional photodynamic therapy is severely constrained by the limited light-penetration depth in tissue. Here, we show efficient photodynamic therapy (PDT) mediated by bioluminescence resonance energy transfer (BRET) that overcomes the light-penetration limitation.
Yingkun Yang +5 more
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Probing Bioluminescence Resonance Energy Transfer in Quantum Rod–Luciferase Nanoconjugates
ACS Nano, 2016We describe the necessary design criteria to create highly efficient energy transfer conjugates containing luciferase enzymes derived from Photinus pyralis (Ppy) and semiconductor quantum rods (QRs) with rod-in-rod (r/r) microstructure. By fine-tuning the synthetic conditions, CdSe/CdS r/r-QRs were prepared with two different emission colors and three ...
Rabeka, Alam +6 more
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Engineering Aspects of Bioluminescence Resonance Energy Transfer Systems
2013Development of optical probes for sensing biological functions in vivo is in high demand in modern biology. With several inherent advantages, the bioluminescence resonance energy transfer (BRET)-based sensors are rapidly expanding and showing great utilities in the study of protein–protein interactions (PPIs), protein dimerization, signal transduction,
Abhijit De, Rohit Arora, Akshi Jasani
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2016
IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA have been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and
Vincent, Compan, Pablo, Pelegrín
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IL-1β processing is one of the hallmarks of inflammasome activation and drives the initiation of the inflammatory response. For decades, Western blot or ELISA have been extensively used to study this inflammatory event. Here, we describe the use of a bioluminescence resonance energy transfer (BRET) biosensor to monitor IL-1β processing in real time and
Vincent, Compan, Pablo, Pelegrín
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Analysis of Arrestin Recruitment to Chemokine Receptors by Bioluminescence Resonance Energy Transfer
2016Chemokine receptors recruit the multifunctional scaffolding protein beta arrestin in response to binding of their chemokine ligands. Given that arrestin recruitment represents a signaling axis that is in part independent from G-protein signaling, it has become a hallmark of G protein-coupled receptor functional selectivity. Therefore, quantification of
J, Bonneterre +4 more
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Using Bioluminescence Resonance Energy Transfer to Measure Ion Channel Assembly
2008Bioluminescence Resonance Energy Transfer (BRET) measures protein interactions within 10 nm of each other. Aside from its ability to probe for interactions at high resolution, this technique operates in live, intact cells, and offers a high throughput method of detection. Thus far, BRET has been widely used in measuring G protein receptor dimerization.
Gina M, Whitaker, Eric A, Accili
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Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling
2015Bioluminescence resonance energy transfer (BRET) is a well-established technique for investigating G protein-coupled receptor (GPCR) pharmacology. BRET enables the monitoring of molecular proximity through the use of heterologously expressed proteins of interest and/or fluorophore-labeled ligands.
Elizabeth K M, Johnstone +1 more
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Bioanalytical Systems Based on Bioluminescence Resonance Energy Transfer Using Firefly Luciferase
Combinatorial Chemistry & High Throughput Screening, 2015Bioanalytical systems based on the Bioluminescence Resonance Energy Transfer (BRET) are widely used in fundamental biochemical studies, as well as for screening and analysis of biologically active compounds. The Renilla luciferase is the most often used energy donor in this system despite the fact that it has low bioluminescence quantum yield and ...
Darya V, Smirnova, Natalia N, Ugarova
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Molecular Pharmacology, 2001
We have developed a procedure based on bioluminescence resonance energy transfer (BRET) to monitor the activation state of the insulin receptor in vitro. Human insulin receptor cDNA was fused to either Renilla luciferase (Rluc) or enhanced yellow fluorescent protein (EYFP) coding sequences. Fusion insulin receptors were partially purified by wheat-germ
N, Boute, K, Pernet, T, Issad
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We have developed a procedure based on bioluminescence resonance energy transfer (BRET) to monitor the activation state of the insulin receptor in vitro. Human insulin receptor cDNA was fused to either Renilla luciferase (Rluc) or enhanced yellow fluorescent protein (EYFP) coding sequences. Fusion insulin receptors were partially purified by wheat-germ
N, Boute, K, Pernet, T, Issad
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