Results 191 to 200 of about 8,042,386 (212)

Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization

Current Opinion in Pharmacology, 2010
The field of G protein-coupled receptor (GPCR) research has undergone a transformation in recent years due to the notion of heteromerization. In order to progress our understanding of the functional implications of this phenomenon, as well as its applicability across the diversity of GPCR subtypes, we need to continually look to improve the ...
Mohammed A, Ayoub, Kevin D G, Pfleger
openaire   +2 more sources

Dimerization of the melanocortin 4 receptor: A study using bioluminescence resonance energy transfer

Peptides, 2006
The melanocortin 4 receptor is important in the regulation of satiety. In this study we have investigated the propensity of the MC4 receptor to homodimerize. MC4 receptors with either a modified green fluorescent protein (GFP(2)) or Renilla luciferase (RLuc) at their C-terminus were constructed.
Sarah A, Nickolls, Rich A, Maki
openaire   +2 more sources

Glycoprotein-glycoprotein Receptor Binding Detection Using Bioluminescence Resonance Energy Transfer

Endocrinology
Abstract The glycoprotein receptors, members of the large G protein-coupled receptor family, are characterized by a large extracellular domains responsible for binding their glycoprotein hormones. Hormone-receptor interactions are traditionally analyzed by ligand-binding assays, most often using radiolabeling but also by thermal shift
Kamila Adamczuk, Thu Ha Ngo, Jakub Czapiński, Adolfo Rivero-Müller   +3 more
openaire   +3 more sources

Analysis of Protein–Protein Interactions Using Bioluminescence Resonance Energy Transfer

2009
Knowledge of how and when proteins interact in living cells is fundamental to our understanding of cellular biology, and bioluminescence resonance energy transfer (BRET) provides an increasingly popular mechanism for studying these interactions in real time.
openaire   +2 more sources

Detection of ERα-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer

2009
Bioluminescent Resonance Energy Transfer is a naturally occurring phenomenon that can be exploited to explore protein-protein interactions in real-time in intact cells and cellular extracts. It detects energy transferred between a bioluminescent donor enzyme (Renilla luciferase) fusion protein and a fluorescent (GFP(2), a mutant of Green Fluorescent ...
Tamika T, Duplessis, Kristen L, Koterba, Brian G, Rowan   +2 more
openaire   +2 more sources

Firefly Luciferase-Based Sequential Bioluminescence Resonance Energy Transfer (BRET)-Fluorescence Resonance Energy Transfer (FRET) Protease Assays

2016
We describe here the preparation of ratiometric luminescent probes that contain two well-separated emission peaks produced by a sequential bioluminescence resonance energy transfer (BRET)-fluorescence resonance energy transfer (FRET) process. The probes are single soluble fusion proteins consisting of a thermostable firefly luciferase variant that ...
openaire   +2 more sources

Detection of protein-protein interaction by bioluminescence resonance energy transfer from firefly luciferase to red fluorescent protein

Journal of Bioscience and Bioengineering, 2002
Ryoichi Arai, Hiroshi Ueda, Teruyuki Nagamune   +2 more
exaly   +2 more sources

Quantum Dot/Bioluminescence Resonance Energy Transfer Based Highly Sensitive Detection of Proteases†

Angewandte Chemie - International Edition, 2007
Jianghong Rao, Zuyong Xia
exaly  

Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells.

Journal of Biomolecular Screening, 2008
Kevin Pfleger, Ruth Seeber, Karin A Eidne   +2 more
exaly  

Development of an RNA detection system using bioluminescence resonance energy transfer

Sensors and Actuators B: Chemical, 2011
Tamaki Endoh, Masayasu Mie
exaly