Results 1 to 10 of about 14,160 (163)

Improved HaloTag Ligand Enables BRET Imaging With NanoLuc

Frontiers in Chemistry, 2020
Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions.
Congrong Wang, Eduard Fron, Susana Rocha   +2 more
exaly   +3 more sources

Developments in FRET- and BRET-Based Biosensors

Micromachines, 2022
Resonance energy transfer technologies have achieved great success in the field of analysis. Particularly, fluorescence resonance energy transfer (FRET) and bioluminescence resonance energy transfer (BRET) provide strategies to design tools for sensing ...
Yuexin Wu, Tianyu Jiang
exaly   +3 more sources

BRET biosensors to study GPCR biology, pharmacology, and signal transduction

Frontiers in Endocrinology, 2012
Bioluminescence Resonance Energy Transfer (BRET) based biosensors have been extensively used over the last decade to study protein-protein interactions and intracellular signal transduction in living cells.
Ali Salahpour, Stefano Espinoza, Bernard Masri   +2 more
exaly   +3 more sources

Novel BRET combination for detection of rapamycin-induced protein dimerization using luciferase from fungus Neonothopanus nambi

Heliyon
Bioluminescence resonance energy transfer (BRET) is one of the most promising approaches used for noninvasive imaging of protein-protein interactions in vivo. Recently, our team has discovered a genetically encodable bioluminescent system from the fungus
Shalini Dimri, Nadezhda M Markina, Karen S Sarkisyan   +2 more
exaly   +3 more sources

Construction of an Enzymatically-Conjugated DNA Aptamer–Protein Hybrid Molecule for Use as a BRET-Based Biosensor

Applied Sciences (Switzerland), 2020
DNA-protein conjugates are useful molecules for construction of biosensors. Herein, we report the development of an enzymatically-conjugated DNA aptamer–protein hybrid molecule for use as a bioluminescence resonance energy transfer (BRET)-based biosensor.
Masayasu Mie, Yasumasa Mashimo, Eiry Kobatake   +2 more
exaly   +3 more sources

Optimization of BRET saturation assays for robust and sensitive cytosolic protein–protein interaction studies

Scientific Reports, 2022
Bioluminescence resonance energy transfer (BRET) saturation is a method of studying protein–protein interaction (PPI) upon quantification of the dependence of the BRET signal on the acceptor/donor (A:D) expression ratio.
Benoit Besson, Hyeju Eun, Seonhee Kim, Marc P. Windisch, Herve Bourhy, Regis Grailhe   +5 more
doaj   +1 more source

Unraveling Receptor Stoichiometry Using Bret [PDF]

Frontiers in Endocrinology, 2012
The first and arguably most important question that could be asked about the biology of any protein is: does it function alone? Cell surface receptors present special problems for stoichiometric analysis because, being located within lipid bilayers, they are often very hydrophobic, which means that once isolated they can exhibit a strong tendency to ...
Felce, J, Davis, S
openaire   +4 more sources

Application of bioluminescence resonance energy transfer-based cell tracking approach in bone tissue engineering

Journal of Tissue Engineering, 2021
Bioluminescent imaging (BLI) has emerged as a popular in vivo tracking modality in bone regeneration studies stemming from its clear advantages: non-invasive, real-time, and inexpensive. We recently adopted bioluminescence resonance energy transfer (BRET)
Lufei Wang, Dong Joon Lee, Han Han, Lixing Zhao, Hiroshi Tsukamoto, Yong-IL Kim, Adele M Musicant, Kshitij Parag-Sharma, Xiangxiang Hu, Henry C Tseng, Jen-Tsan Chi, Zhengyan Wang, Antonio L Amelio, Ching-Chang Ko   +13 more
doaj   +1 more source

Ratiometric Detection of Zn²⁺ Using DNAzyme-Based Bioluminescence Resonance Energy Transfer Sensors

Chemistry, 2023
While fluorescent sensors have been developed for monitoring metal ions in health and diseases, they are limited by the requirement of an excitation light source that can lead to photobleaching and a high autofluorescence background.
Yuting Wu, Whitney Lewis, Jing Luen Wai, Mengyi Xiong, Jiao Zheng, Zhenglin Yang, Chloe Gordon, Ying Lu, Siu Yee New, Xiao-Bing Zhang, Yi Lu   +10 more
doaj   +1 more source

Analysis of RXR/THR and RXR/PPARG2 heterodimerization by bioluminescence resonance energy transfer (BRET). [PDF]

PLoS ONE, 2013
BACKGROUND: Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death.
Miquel Mulero, Julie Perroy, Carole Federici, Gérard Cabello, Vincent Ollendorff   +4 more
doaj   +1 more source