Results 1 to 10 of about 5,198 (113)
A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In Vivo [PDF]
Journal of Extracellular VesiclesExtracellular vesicles (EVs) affect the function of cells in living animals. Yet, cell type‐specific EV abundances and their distribution in biological fluids are technically challenging to study.
Didde Riisager Hansen +3 more
doaj +2 more sources
A cost-effective dual reporter system in Nicotiana benthamiana [PDF]
Frontiers in Plant ScienceDual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening ...
Zeru Zhang +11 more
doaj +2 more sources
A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases [PDF]
Applied Microbiology and BiotechnologyConventional immunoassays such as ELISA are widely used in serological testing, yet their reliance on multi-step workflows and labeled reagents limits diagnostic scalability and speed.
Xuesai Li +9 more
doaj +2 more sources
NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening
Frontiers in Pharmacology, 2016 Based on the recent development of NanoLuc Luciferase a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic ...
Peter Lowe +2 more
exaly +3 more sources
Improved HaloTag Ligand Enables BRET Imaging With NanoLuc
Frontiers in Chemistry, 2020 Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions.
Luke Lavis +2 more
exaly +3 more sources
Development of a Ready‐To‐Use Bioluminescence Immunosensor for the One‐Step Sensitive Detection of Antibodies Against African Swine Fever Virus [PDF]
Microbial BiotechnologyRapid and reliable analytical techniques play important roles in various research fields and are particularly crucial for diagnosing infectious diseases in clinical settings.
Zhonghui Zhang +10 more
doaj +2 more sources
Frontiers in Bioengineering and Biotechnology, 2021 Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca++ which has been a focus of using genetically encoded Ca++ indicators (GECIs ...
Jie Yang, Carl Hirschie Johnson
doaj +1 more source
Frontiers in Veterinary Science, 2022 H9N2 avian influenza viruses (AIVs) continuously cross the species barrier to infect mammalians and are repeatedly transmitted to humans, posing a significant threat to public health.
Guangjie Lao +17 more
doaj +1 more source
Communications Biology, 2023 Transgenic luciferase-expressing Plasmodium falciparum parasites have been widely used for the evaluation of anti-malarial compounds. Here, to screen for anti-malarial drugs effective against multiple stages of the parasite, we generate a P.
Yukiko Miyazaki +11 more
doaj +1 more source
Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays. [PDF]
PLoS Neglected Tropical Diseases, 2018 The protozoan parasite Leishmania causes leishmaniasis; a spectrum of diseases of which there are an estimated 1 million new cases each year. Current treatments are toxic, expensive, difficult to administer, and resistance to them is emerging.
Sarah L Berry +6 more
doaj +1 more source