NanoLuc Luciferase – A Multifunctional Tool for High Throughput Antibody Screening [PDF]
Frontiers in Pharmacology, 2016 Based on the recent development of NanoLuc Luciferase a small (19 kDa), highly stable, ATP independent, bioluminescent protein, an extremely robust and ultra high sensitivity screening system has been developed whereby primary hits of therapeutic ...
Nicolas Boute +2 more
exaly +6 more sources
Improved HaloTag Ligand Enables BRET Imaging With NanoLuc [PDF]
Frontiers in Chemistry, 2020 Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions.
Congrong Wang +2 more
exaly +7 more sources
Intracellular Ionic Strength Sensing Using NanoLuc [PDF]
International Journal of Molecular Sciences, 2021 Intracellular ionic strength regulates myriad cellular processes that are fundamental to cellular survival and proliferation, including protein activity, aggregation, phase separation, and cell volume. It could be altered by changes in the activity of cellular signaling pathways, such as those that impact the activity of membrane-localized ion channels
Tausif Altamash +2 more
exaly +4 more sources
Expanding the bioluminescent reporter toolkit for plant science with NanoLUC [PDF]
Plant Methods, 2019 Background Protein data over circadian time scale is scarce for clock transcription factors. Further work in this direction is required for refining quantitative clock models.
Uriel Urquiza-García, Andrew J. Millar
doaj +6 more sources
Engineering and exploiting synthetic allostery of NanoLuc luciferase [PDF]
Nature Communications, 2022 Allosteric regulation of proteins allows transduction and interconversion of biochemical signals. Here the authors construct an allosteric version of circular permutated NanoLuc that can be activated by a ligand; they use this to create biosensors to ...
Zhong Guo +10 more
doaj +6 more sources
A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In Vivo [PDF]
Journal of Extracellular VesiclesExtracellular vesicles (EVs) affect the function of cells in living animals. Yet, cell type‐specific EV abundances and their distribution in biological fluids are technically challenging to study.
Didde Riisager Hansen +3 more
doaj +2 more sources
A cost-effective dual reporter system in Nicotiana benthamiana [PDF]
Frontiers in Plant ScienceDual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening ...
Zeru Zhang +11 more
doaj +2 more sources
A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases [PDF]
Applied Microbiology and BiotechnologyConventional immunoassays such as ELISA are widely used in serological testing, yet their reliance on multi-step workflows and labeled reagents limits diagnostic scalability and speed.
Xuesai Li +9 more
doaj +2 more sources
Listeria innocua Biofilm Assay Using NanoLuc Luciferase
Bio-Protocol, 2022 Biofilms serve as a bacterial survival strategy, allowing bacteria to persist under adverse environmental conditions. The non-pathogenic Listeria innocua is used as a surrogate organism for the foodborne pathogen Listeria monocytogenes, because they ...
Aleš Berlec +4 more
doaj +3 more sources
Development of a Ready‐To‐Use Bioluminescence Immunosensor for the One‐Step Sensitive Detection of Antibodies Against African Swine Fever Virus [PDF]
Microbial BiotechnologyRapid and reliable analytical techniques play important roles in various research fields and are particularly crucial for diagnosing infectious diseases in clinical settings.
Zhonghui Zhang +10 more
doaj +2 more sources