A versatile Plasmodium falciparum reporter line expressing NanoLuc enables highly sensitive multi-stage drug assays [PDF]
Communications Biology, 2023 Transgenic luciferase-expressing Plasmodium falciparum parasites have been widely used for the evaluation of anti-malarial compounds. Here, to screen for anti-malarial drugs effective against multiple stages of the parasite, we generate a P.
Yukiko Miyazaki +11 more
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NanoLuc-based microneutralization assay for high-throughput detection of neutralizing antibodies against rift valley fever virus [PDF]
MethodsXPlaque reduction neutralization tests (PRNTs) are the gold standard for measuring neutralizing antibodies but are labor-intensive, low-throughput, and subject to variability from manual plaque counting.
Cigdem Alkan +2 more
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A Sensitive Reporter Mouse Model to Study Adipocyte‐Derived Extracellular Vesicles In Vivo [PDF]
Journal of Extracellular VesiclesExtracellular vesicles (EVs) affect the function of cells in living animals. Yet, cell type‐specific EV abundances and their distribution in biological fluids are technically challenging to study.
Didde Riisager Hansen +3 more
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A cost-effective dual reporter system in Nicotiana benthamiana [PDF]
Frontiers in Plant ScienceDual reporter assay has been widely used for quantification of gene expression in various model systems. However, the assay is dependent on commercial kits, which may be inhibitory for many low-budget academic laboratories or high throughput screening ...
Zeru Zhang +11 more
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Development of NanoLuc-PEST expressing Leishmania mexicana as a new drug discovery tool for axenic- and intramacrophage-based assays. [PDF]
PLoS Neglected Tropical Diseases, 2018 The protozoan parasite Leishmania causes leishmaniasis; a spectrum of diseases of which there are an estimated 1 million new cases each year. Current treatments are toxic, expensive, difficult to administer, and resistance to them is emerging.
Sarah L Berry +6 more
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A broadly applicable split-luciferase biosensor approach for rapid antibody detection in emerging infectious diseases [PDF]
Applied Microbiology and BiotechnologyConventional immunoassays such as ELISA are widely used in serological testing, yet their reliance on multi-step workflows and labeled reagents limits diagnostic scalability and speed.
Xuesai Li +9 more
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Frontiers in Bioengineering and Biotechnology, 2021 Sensitive detection of biological events is a goal for the design and characterization of sensors that can be used in vitro and in vivo. One important second messenger is Ca++ which has been a focus of using genetically encoded Ca++ indicators (GECIs ...
Jie Yang, Carl Hirschie Johnson
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Frontiers in Veterinary Science, 2022 H9N2 avian influenza viruses (AIVs) continuously cross the species barrier to infect mammalians and are repeatedly transmitted to humans, posing a significant threat to public health.
Guangjie Lao +17 more
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Cải thiện cảm biến sinh học nhận diện nhóm kháng sinh ức chế sự tổng hợp protein của vi khuẩn
Tạp chí Khoa học Đại học Cần Thơ, 2023 Nghiên cứu được thực hiện nhằm cải thiện độ nhạy của cảm biến sinh học nhận diện nhóm kháng sinh ức chế sự tổng hợp protein trên nền giấy. Cảm biến sinh học nhận diện kháng sinh dựa trên cường độ phát quang của protein nanoluciferase (NanoLuc) được tổng
Trần Thị Mỹ Duyên +1 more
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Retrovirology, 2020 Background The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus.
James Kirui, Eric O. Freed
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