Results 41 to 50 of about 5,929 (191)

Thermostable Bioluminescent Intercalating Dyes for Real‐Time, Integrated Nucleic Acid Amplification and Detection

Angewandte Chemie, EarlyView.
We present a bioluminescent diagnostic platform that integrates DNA amplification and detection in a single step. The engineering of thermostable luciferase‐intercalating dye conjugates and controlled release of caged luciferin substrates enable real‐time, detection of attomolar concentrations of viral DNA within 30 min.
Yosta de Stigter, Harmen J. van der Veer, Sterre de Lignie, Robbert J. de Haas, Renko de Vries, Joost P. H. Schoeber, Anne J. M. Loonen, Adriaan J. C. van den Brule, Maarten Merkx   +8 more
wiley   +2 more sources

Analysis of RAS and drug induced homo- and heterodimerization of RAF and KSR1 proteins in living cells using split Nanoluc luciferase

Cell Communication and Signaling, 2023
The dimerization of RAF kinases represents a key event in their activation cycle and in RAS/ERK pathway activation. Genetic, biochemical and structural approaches provided key insights into this process defining RAF signaling output and the clinical ...
Lino Rohrer, Corinna Spohr, Carina Beha, Ricarda Griffin, Sandra Braun, Sebastian Halbach, Tilman Brummer   +6 more
doaj   +1 more source

Dual Reporter Bioluminescence Imaging with NanoLuc and Firefly Luciferase [PDF]

, 2018
Bioluminescence imaging is a powerful, broadly utilized method for noninvasive imaging studies in cell-based assays and small animal models of normal physiology and multiple diseases. In combination with molecular engineering of cells and entire organisms using luciferase enzymes, bioluminescence imaging has enabled novel applications including studies
Anne E, Gibbons, Kathryn E, Luker, Gary D, Luker   +2 more
openaire   +2 more sources

Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle

Malaria Journal, 2021
Background Plasmodium knowlesi is now the major cause of human malaria in Malaysia, complicating malaria control efforts that must attend to the elimination of multiple Plasmodium species. Recent advances in the cultivation of P.
Roberto R. Moraes Barros, Kittisak Thawnashom, Tyler J. Gibson, Jennifer S. Armistead, Ramoncito L. Caleon, Miho Kaneko, Whitney A. Kite, J. Patrick Mershon, Jacqueline K. Brockhurst, Theresa Engels, Lynn Lambert, Sachy Orr-Gonzalez, John H. Adams, Juliana M. Sá, Osamu Kaneko, Thomas E. Wellems   +15 more
doaj   +1 more source

An ultrasensitive NanoLuc-based luminescence system for monitoring Plasmodium berghei throughout its life cycle [PDF]

, 2016
BACKGROUND: Bioluminescence imaging is widely used for cell-based assays and animal imaging studies, both in biomedical research and drug development.
Derya Keller, Wacker, Rahel Corina, De Niz Hidalgo, Mariana Isabel, Rebecca R. Stanway, De Niz, Mariana, Heussler, Volker T., Limenitakis Stanway, Rebecca, Keller, Derya, Volker T. Heussler, Stanway, Rebecca R., Wacker, Rahel, Mariana De Niz, Rahel Wacker   +12 more
core   +1 more source

Luciferase complementation based-detection of G-protein-coupled receptor activity

BioTechniques, 2018
Protein complementation assays (PCA) are used as pharmacological tools, enabling a wide array of applications, ranging from studies of protein–protein interactions to second messenger effects.
Hideaki Yano, Ning Sheng Cai, Jonathan A Javitch, Sergi Ferré   +3 more
doaj   +1 more source

Mechanical Unfolding and Refolding of NanoLuc via Single-Molecule Force Spectroscopy and Computer Simulations

, 2022
A highly bioluminescent protein, NanoLuc (Nluc), has seen numerous applications in biological assays since its creation. We recently engineered a NanoLuc polyprotein that showed high bioluminescence but displayed a strong misfolding propensity after ...
Dimitra Apostolidou (14093202), Pan Zhang (206408), Piotr E. Marszalek (101957), Weitao Yang (838638)   +3 more
core   +3 more sources

Ratiometric BRET Measurements of ATP with a Genetically-Encoded Luminescent Sensor

Sensors, 2019
Luciferase-based reporters provide a key measurement approach in a broad range of applications, from in vitro high-throughput screening to whole animal imaging.
Se-Hong Min, Alexander R. French, Keelan J. Trull, Kiet Tat, S. Ashley Varney, Mathew Tantama   +5 more
doaj   +1 more source

Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase.

PLoS ONE, 2023
Mutagens and oxidative agents damage biomolecules, such as DNA; therefore, detecting genotoxic and oxidative chemicals is crucial for maintaining human health.
Minami Shichinohe, Shun Ohkawa, Yuu Hirose, Toshihiko Eki   +3 more
doaj   +1 more source

NanoLuc Bioluminescence-Driven Photodynamic Activation of Cholecystokinin 1 Receptor with Genetically-Encoded Protein Photosensitizer MiniSOG

, 2020
In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated by singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini singlet oxygen ...
Zong Jie Cui, Yuan Li
core   +1 more source