Results 41 to 50 of about 6,439 (202)

Activity of Plasmodium vivax promoter elements in Plasmodium knowlesi, and a centromere-containing plasmid that expresses NanoLuc throughout the parasite life cycle

Malaria Journal, 2021
Background Plasmodium knowlesi is now the major cause of human malaria in Malaysia, complicating malaria control efforts that must attend to the elimination of multiple Plasmodium species. Recent advances in the cultivation of P.
Roberto R. Moraes Barros, Kittisak Thawnashom, Tyler J. Gibson, Jennifer S. Armistead, Ramoncito L. Caleon, Miho Kaneko, Whitney A. Kite, J. Patrick Mershon, Jacqueline K. Brockhurst, Theresa Engels, Lynn Lambert, Sachy Orr-Gonzalez, John H. Adams, Juliana M. Sá, Osamu Kaneko, Thomas E. Wellems   +15 more
doaj   +1 more source

Analysis of RAS and drug induced homo- and heterodimerization of RAF and KSR1 proteins in living cells using split Nanoluc luciferase

Cell Communication and Signaling, 2023
The dimerization of RAF kinases represents a key event in their activation cycle and in RAS/ERK pathway activation. Genetic, biochemical and structural approaches provided key insights into this process defining RAF signaling output and the clinical ...
Lino Rohrer, Corinna Spohr, Carina Beha, Ricarda Griffin, Sandra Braun, Sebastian Halbach, Tilman Brummer   +6 more
doaj   +1 more source

Differential Water Networks Guide Selectivity Optimization of a Cell Active BPTF Inhibitor in Neuroblastoma

Angewandte Chemie, EarlyView.
BZ2 is a second‐generation Bromodomain PHD finger Transcription Factor (BPTF) inhibitor with improved selectivity over Class I and Class IV BRD off‐targets identified in our previously reported inhibitor, BZ1. Structural analyses reveal that water network engagement is key to this selectivity.
Kesavan Babu, Chun‐Ju Tsou, Sourav Das, Liying Fan, Anasuya Pal, Molly Sneddon, Timothy R. Stachowski, Priyanka Samanta, Stanley Nithianantham, Caroline Buchholz, Shi Zhang, Xiang Fu, Rahul S. Kathayat, Wenwei Lin, Yongtao Li, Lei Yang, Taosheng Chen, Marcus Fischer, Anang A. Shelat, William C. K. Pomerantz   +19 more
wiley   +2 more sources

Luciferase complementation based-detection of G-protein-coupled receptor activity

BioTechniques, 2018
Protein complementation assays (PCA) are used as pharmacological tools, enabling a wide array of applications, ranging from studies of protein–protein interactions to second messenger effects.
Hideaki Yano, Ning Sheng Cai, Jonathan A Javitch, Sergi Ferré   +3 more
doaj   +1 more source

Sensing chemical-induced genotoxicity and oxidative stress via yeast-based reporter assays using NanoLuc luciferase.

PLoS ONE, 2023
Mutagens and oxidative agents damage biomolecules, such as DNA; therefore, detecting genotoxic and oxidative chemicals is crucial for maintaining human health.
Minami Shichinohe, Shun Ohkawa, Yuu Hirose, Toshihiko Eki   +3 more
doaj   +1 more source

Ratiometric BRET Measurements of ATP with a Genetically-Encoded Luminescent Sensor

Sensors, 2019
Luciferase-based reporters provide a key measurement approach in a broad range of applications, from in vitro high-throughput screening to whole animal imaging.
Se-Hong Min, Alexander R. French, Keelan J. Trull, Kiet Tat, S. Ashley Varney, Mathew Tantama   +5 more
doaj   +1 more source

NanoLuc reporters identify COL4A5 nonsense mutations susceptible to drug-induced stop codon readthrough

iScience, 2022
Summary: Alport syndrome, a disease of kidney, ear, and eye, is caused by pathogenic variants in the COL4A3, COL4A4, or COL4A5 genes encoding collagen α3α4α5(IV) of basement membranes.
Kohei Omachi, Hirofumi Kai, Michel Roberge, Jeffrey H. Miner   +3 more
doaj   +1 more source

NanoLuc Bioluminescence-Driven Photodynamic Activation of Cholecystokinin 1 Receptor with Genetically-Encoded Protein Photosensitizer MiniSOG

, 2020
In contrast to reversible activation by agonist, cholecystokinin 1 receptor (CCK1R) is permanently activated by singlet oxygen generated in photodynamic action, with sulphonated aluminium phthalocyanine or genetically encoded mini singlet oxygen ...
Zong Jie Cui, Yuan Li
core   +1 more source

Internal light source for deep photodynamic therapy

Light: Science & Applications, 2022
Photodynamic therapy (PDT) for deep-seated lesion is seriously hindered by the limited depth of visible light penetration. Most recently, researchers have designed a genetically-encoded NanoLuc-miniSOG with internal light source for self-excitation ...
Buhong Li, Li Lin
doaj   +1 more source

The C‐terminal truncated splicing variant of NK1R negatively modulates substance P‐stimulated NK1R signaling

FEBS Open Bio, EarlyView.
The neurokinin 1 receptor exists as full‐length (NK1L) and C‐terminally truncated (NK1S) splice variants. We show that NK1S heterodimerizes with NK1L, impairing Gαq coupling and Ca2+ mobilization while enhancing β‐arrestin1 recruitment. NK1S suppresses substance P‐driven gene expression and cell migration, revealing NK1S as an endogenous biased ...
Lan Phuong Nguyen, Duc Trung Nguyen, Minyoung Cho, Jihun Kim, Soyeon In, Thai Uy Nguyen, Sunghoon Hurh, Beom Jin Park, Jong‐Ik Hwang   +8 more
wiley   +1 more source