Results 41 to 50 of about 5,275 (178)
Antioxidants, 2023 With the rising prevalence of obesity, non-alcoholic fatty liver disease (NAFLD) now affects 20–25% of the global population. NAFLD, a progressive condition associated with oxidative stress, can result in cirrhosis and liver cancer in 10% and 3% of ...
James Li +5 more
doaj +1 more source
Advanced Healthcare Materials, EarlyView.We developed a patient‐derived, functional microfluidic model of the diffuse midline glioma (DMG) blood–brain–tumor barrier (BBTB) comprised of endothelial cells, astrocytes, pericytes, and tumor cells. The system forms perfusable microvasculature, reveals the BBTB retains vascular integrity, identifies DMG‐specific transcriptomic changes distinct from
Kimberly R. Bennett +7 more
wiley +1 more source
A genetically encoded BRET-based SARS-CoV-2 Mpro protease activity sensor
Communications Chemistry, 2022 The main protease (Mpro) of SARS-CoV-2 is an important target for COVID-19 therapy. Here, a pair of genetically encoded BRET-based sensors for detecting Mpro activity are generated by sandwiching N-terminal autocleavage sites in between the mNeonGreen ...
Anupriya M. Geethakumari +6 more
doaj +1 more source
Evidence for the extracellular delivery of influenza NS1 protein
Microbiology Independent Research Journal, 2021 We constructed a reporter influenza A/Puerto Rico/8/1934 virus expressing truncated 124aa N-terminal NS1 protein fused to a luciferase reporter sequence (NanoLuc) without signal peptide.
Anastasia A. Pulkina +4 more
doaj +1 more source
Synthetic Toll‐Like Receptors for Control of Innate Immunity With Far‐Red Light
Advanced Science, EarlyView.Synthetic Toll‐like receptors (TLRs) are engineered by replacing their natural sensing regions with a far‐red‐light‐responsive module from a bacterial phytochrome. These synthetic receptors enable light‐controlled regulation of immune signaling in mammalian cells.
Anna V. Leopold, Vladislav V. Verkhusha
wiley +1 more source
A probabilistic view of protein stability, conformational specificity, and design
Scientific Reports, 2023 Various approaches have used neural networks as probabilistic models for the design of protein sequences. These "inverse folding" models employ different objective functions, which come with trade-offs that have not been assessed in detail before.
Jacob A. Stern +8 more
doaj +1 more source
Versatile Detection of Cellular Protein via Fluorescence Anisotropy
Advanced Science, EarlyView.Cell Lysate Fluorescence Anisotropy (CFAST) utilizes long lifetime dye‐labeled nanobodies for rapid protein quantification in minimally purified, complex cell lysate. When coupled with cellular thermal shift, CFAST allows high‐throughput detection of endogenous protein‐small molecule engagement, facilitating the discovery of novel binders for ...
Qing Tang +12 more
wiley +1 more source
Coelenterazine analogues emit red-shifted bioluminescence with NanoLuc
Org. Biomol. Chem., 2017 Synthesis and biological investigation of coelenterazine analogs that demonstrate a tunable red-shift in their bioluminescent emission with NanoLuc luciferase.
Anton Shakhmin +5 more
openaire +5 more sources
MethodsXPlaque reduction neutralization tests (PRNTs) are the gold standard for measuring neutralizing antibodies but are labor-intensive, low-throughput, and subject to variability from manual plaque counting.
Cigdem Alkan +2 more
doaj +1 more source
Advanced Science, EarlyView.Inhibition of KDELR2 in a small fraction of tumor cells generates sustainable immunogenic cell death conditions within the tumor microenvironment. These conditions promote the regression of tumors in a T cell independent manner. During regression, macrophages prime T cells that subsequently provide systemic protection against recurrence. The potency of
Shakti P Pattanayak +6 more
wiley +1 more source